Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200121 |
| Name | oriT_ICEValA056-2  |
| Organism | Vibrio alginolyticus strain A056 sequence |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | KR231689 (3488..3786 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 45..50, 52..57 (CAAACG..CGTTTG) 7..12, 26..31 (TTTGGC..GCCAAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEValA056-2
GCTCTGTTTGGCGGCGGATGAATGAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGACGATACGTCTTTTTAGGCGTTTTTAAGTGAAATCGGGCTGTATCCCTTGTCAGATATGGGATTGCGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGCTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCGGATGAATGAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGACGATACGTCTTTTTAGGCGTTTTTAAGTGAAATCGGGCTGTATCCCTTGTCAGATATGGGATTGCGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGCTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14274 | GenBank | ALJ83463 |
| Name | herA_ICEValA056-2_019_ICEValA056-2 |
UniProt ID | _ |
| Length | 716 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 716 a.a. Molecular weight: 79794.89 Da Isoelectric Point: 5.2898
>ALJ83463.1 bipolar DNA helicase HerA [Vibrio alginolyticus]
MFKNLFFQTKALPELSSQLDTEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWATNNRIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPENVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTNLYLVWKSAAKEIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESFAPEVLIKDSKPIWLPMLHISEADLLFCSNVPSGVTLFNKSEWEATQQTQAEPQSRSSEHPDL
SEASSSIEHSNSAESPSSKSSEQDDELRHASDVSTPQATENAPGDECEKPNNSYDGAISNNVNQQDAEAL
NLPESLAWFPEASSALIMVGEQLLIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTITTKDGV
QEQGLLLKVSISKGLTALIDLSKQDAESVTAIQNKEASQRPSRINITNAQAKEPATRAERKQKPIAANAN
SSTDPKHAQRQQMVSFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAKR
MFKNLFFQTKALPELSSQLDTEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWATNNRIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPENVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTNLYLVWKSAAKEIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESFAPEVLIKDSKPIWLPMLHISEADLLFCSNVPSGVTLFNKSEWEATQQTQAEPQSRSSEHPDL
SEASSSIEHSNSAESPSSKSSEQDDELRHASDVSTPQATENAPGDECEKPNNSYDGAISNNVNQQDAEAL
NLPESLAWFPEASSALIMVGEQLLIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTITTKDGV
QEQGLLLKVSISKGLTALIDLSKQDAESVTAIQNKEASQRPSRINITNAQAKEPATRAERKQKPIAANAN
SSTDPKHAQRQQMVSFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAKR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16533 | GenBank | ALJ83462 |
| Name | tcpA_ICEValA056-2_018_ICEValA056-2 |
UniProt ID | _ |
| Length | 577 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 577 a.a. Molecular weight: 63396.86 Da Isoelectric Point: 7.0313
>ALJ83462.1 hypothetical protein ICEValA056-2_018 [Vibrio alginolyticus]
MERKNWFSPYDPTRRIGSVISVSATELIINLSKAGSGEPSWSFGNRIAAGEVNEFVFIDIGETAILGRLV
KVWIEGGDRLSVDGLADKPSENHPIGLVQLLVSLNPANGRNYKGIKQHPRLGSQIYSAHPYLVSIIAEGK
QDEEPGQIHLPLASLPHDESVTVHVTPEKLFSRHCAILGATGGGKSYSMTNIIEQVIKAGGKAIIIDPTG
EYESLGCDSYYVGNHAKATPFNQLTFPHWQFTDSDIRAFLRPSAQSQAPKLEAAIESQRLVSHYYQNNSF
GLGITSEYLLNKSGQLKATYKSALVQMNSHNQASPWSFALTAEQIECECVYPTANYGKDPSKWGDRSEAD
VGHCLNLISRMKAYASNTNLKWMIDAAGELKTLPDLIDEFCSSNQNNNVIRLDLSAVPFEANSREILVNA
IGRKLLSVARQGAINHGAPLLVFIDEAHQFLNKRIGEETNRFELDAFGNIAKEGRKYGLNTIIATQRPRD
IPEDVLSQIGTLIVHRLTNQLDQEIVKKAVGAIDQRSASFLPVLGQGEALLLGVDFPFPMTVKMKRPANA
PTSKSAGFSKSWNPFQN
MERKNWFSPYDPTRRIGSVISVSATELIINLSKAGSGEPSWSFGNRIAAGEVNEFVFIDIGETAILGRLV
KVWIEGGDRLSVDGLADKPSENHPIGLVQLLVSLNPANGRNYKGIKQHPRLGSQIYSAHPYLVSIIAEGK
QDEEPGQIHLPLASLPHDESVTVHVTPEKLFSRHCAILGATGGGKSYSMTNIIEQVIKAGGKAIIIDPTG
EYESLGCDSYYVGNHAKATPFNQLTFPHWQFTDSDIRAFLRPSAQSQAPKLEAAIESQRLVSHYYQNNSF
GLGITSEYLLNKSGQLKATYKSALVQMNSHNQASPWSFALTAEQIECECVYPTANYGKDPSKWGDRSEAD
VGHCLNLISRMKAYASNTNLKWMIDAAGELKTLPDLIDEFCSSNQNNNVIRLDLSAVPFEANSREILVNA
IGRKLLSVARQGAINHGAPLLVFIDEAHQFLNKRIGEETNRFELDAFGNIAKEGRKYGLNTIIATQRPRD
IPEDVLSQIGTLIVHRLTNQLDQEIVKKAVGAIDQRSASFLPVLGQGEALLLGVDFPFPMTVKMKRPANA
PTSKSAGFSKSWNPFQN
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16534 | GenBank | ALJ83464 |
| Name | traI_ICEValA056-2_020_ICEValA056-2 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67086.89 Da Isoelectric Point: 6.5728
>ALJ83464.1 conjugative transfer protein TraI [Vibrio alginolyticus]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLHKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLHKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16535 | GenBank | ALJ83478 |
| Name | traC_ICEValA056-2_035_ICEValA056-2 |
UniProt ID | _ |
| Length | 799 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 799 a.a. Molecular weight: 90171.75 Da Isoelectric Point: 5.9735
>ALJ83478.1 IncF plasmid conjugative transfer pilus assembly protein TraC [Vibrio alginolyticus]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 19208..45506
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ICEValA056-2_016 | 14716..17832 | + | 3117 | ALJ83460 | anticodon nuclease | - |
| ICEValA056-2_017 | 17936..19195 | + | 1260 | ALJ83461 | type I restriction-modification system restriction subunit R | - |
| ICEValA056-2_018 | 19208..20941 | + | 1734 | ALJ83462 | hypothetical protein | virb4 |
| ICEValA056-2_019 | 21059..23209 | + | 2151 | ALJ83463 | bipolar DNA helicase HerA | - |
| ICEValA056-2_020 | 23257..25077 | + | 1821 | ALJ83464 | conjugative transfer protein TraI | virb4 |
| ICEValA056-2_021 | 25087..25647 | + | 561 | ALJ83465 | IncF plasmid conjugative transfer protein TraD | - |
| ICEValA056-2_022 | 25634..26269 | + | 636 | ALJ83466 | conjugative transfer protein 234 | tfc7 |
| ICEValA056-2_023 | 26407..27513 | + | 1107 | ALJ83467 | conjugative transfer protein TraJ | - |
| ICEValA056-2_024 | 27569..27703 | - | 135 | ALJ83551 | Fic family protein | - |
| ICEValA056-2_025 | 27715..28005 | - | 291 | ALJ83468 | HigA antitoxin protein | - |
| ICEValA056-2_026 | 28238..28519 | + | 282 | ALJ83469 | IncF plasmid conjugative transfer pilus assembly protein TraL | traL |
| ICEValA056-2_027 | 28516..29142 | + | 627 | ALJ83470 | IncF plasmid conjugative transfer pilus assembly protein TraE | traE |
| ICEValA056-2_028 | 29126..30022 | + | 897 | ALJ83471 | IncF plasmid conjugative transfer pilus assembly protein TraK | traK |
| ICEValA056-2_029 | 30025..31314 | + | 1290 | ALJ83472 | IncF plasmid conjugative transfer pilus assembly protein TraB | traB |
| ICEValA056-2_030 | 31389..31961 | + | 573 | ALJ83473 | conjugative transfer protein TraV | traV |
| ICEValA056-2_031 | 31958..32344 | + | 387 | ALJ83474 | conjugative transfer protein TraA | - |
| ICEValA056-2_032 | 32522..33355 | + | 834 | ALJ83475 | toxin protein MosT | - |
| ICEValA056-2_033 | 33348..34286 | + | 939 | ALJ83476 | antitoxin protein MosA | - |
| ICEValA056-2_034 | 34418..35110 | + | 693 | ALJ83477 | disulfide isomerase | trbB |
| ICEValA056-2_035 | 35110..37509 | + | 2400 | ALJ83478 | IncF plasmid conjugative transfer pilus assembly protein TraC | virb4 |
| ICEValA056-2_036 | 37588..38691 | - | 1104 | ALJ83479 | transposase | - |
| ICEValA056-2_037 | 38905..39168 | + | 264 | ALJ83480 | conjugative transfer protein 345 | - |
| ICEValA056-2_038 | 39152..39664 | + | 513 | ALJ83481 | conjugative signal peptidase TrhF | - |
| ICEValA056-2_039 | 39675..40799 | + | 1125 | ALJ83482 | IncF plasmid conjugative transfer pilus assembly protein TraW | traW |
| ICEValA056-2_040 | 40831..41811 | + | 981 | ALJ83483 | IncF plasmid conjugative transfer pilus assembly protein TraU | traU |
| ICEValA056-2_041 | 41814..45506 | + | 3693 | ALJ83484 | IncF plasmid conjugative transfer protein TraN | traN |
| ICEValA056-2_042 | 45738..46070 | + | 333 | ALJ83548 | hypothetical protein | - |
| ICEValA056-2_043 | 46101..46790 | + | 690 | ALJ83546 | hypothetical protein | - |
| ICEValA056-2_044 | 46768..47034 | - | 267 | ALJ83485 | mobile element protein | - |
| ICEValA056-2_045 | 47106..47534 | + | 429 | ALJ83486 | mobile element protein | - |
| ICEValA056-2_046 | 47580..48479 | - | 900 | ALJ83487 | transposase | - |
| ICEValA056-2_047 | 48816..49013 | - | 198 | ALJ83550 | hypothetical protein | - |
| ICEValA056-2_048 | 49021..49134 | - | 114 | ALJ83554 | hypothetical protein | - |
Host bacterium
| ID | 123 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEValA056-2 | GenBank | KR231689 |
| Element size | 103826 bp | Coordinate of oriT [Strand] | 3488..3786 [+] |
| Host bacterium | Vibrio alginolyticus strain A056 sequence | Coordinate of element | 1..103826 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIA |