Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200107 |
| Name | oriT_ICEPmiCHN2410  |
| Organism | Proteus mirabilis strain 09MAS2410 sequence |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | KX243406 (4309..4607 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 45..50, 52..57 (CAAACG..CGTTTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEPmiCHN2410
GCTCTGTTTGGCGGCGGATGACCTAGTCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGACGATACGTCGTTTTAGGCGTTTTTAAGTGAAATCGGGCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCGGATGACCTAGTCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGACGATACGTCGTTTTAGGCGTTTTTAAGTGAAATCGGGCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14260 | GenBank | APO16950 |
| Name | traI_-_ICEPmiCHN2410 |
UniProt ID | _ |
| Length | 716 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 716 a.a. Molecular weight: 79650.73 Da Isoelectric Point: 5.5209
>APO16950.1 Conjugative transfer protein TraI, relaxase [Proteus mirabilis]
MFKNLFFQAKTLPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLAIKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSGVTLFSKPEWEATQQTQAEPQSRSSEHSDL
PEASSSIEHSNSAESPSSKSSDQDDEIRLASDVNHLQATENAPGDECEKPNNSYDGAIANKVNQHDAEAL
NLSESLAWLPEASSALVVVGEQILIRYPDAVRHWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNAGV
QEQGLLLKGFVSKGLAALIDLSKQDAESVTAIQNKEASPRSSRTETTSAQAKELATRAERKQKPIAPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
RFDEGETVLFTAHAKR
MFKNLFFQAKTLPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLAIKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSGVTLFSKPEWEATQQTQAEPQSRSSEHSDL
PEASSSIEHSNSAESPSSKSSDQDDEIRLASDVNHLQATENAPGDECEKPNNSYDGAIANKVNQHDAEAL
NLSESLAWLPEASSALVVVGEQILIRYPDAVRHWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNAGV
QEQGLLLKGFVSKGLAALIDLSKQDAESVTAIQNKEASPRSSRTETTSAQAKELATRAERKQKPIAPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
RFDEGETVLFTAHAKR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16506 | GenBank | APO16952 |
| Name | traD_-_ICEPmiCHN2410 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 67677.58 Da Isoelectric Point: 6.6443
>APO16952.1 IncF plasmid conjugative transfer protein TraD [Proteus mirabilis]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQMG
LKGKPLAFMALAHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHASYRKNSQMGSTW
IHGIEPKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACI
AAGSPERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTS
QRPDLKTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAAN
TDLEGLLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGL
DSLTDAMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIAT
QTFADFAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTG
NHGERLMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQMG
LKGKPLAFMALAHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHASYRKNSQMGSTW
IHGIEPKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACI
AAGSPERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTS
QRPDLKTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAAN
TDLEGLLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGL
DSLTDAMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIAT
QTFADFAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTG
NHGERLMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16507 | GenBank | APO16966 |
| Name | traC_-_ICEPmiCHN2410 |
UniProt ID | _ |
| Length | 799 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 799 a.a. Molecular weight: 90181.69 Da Isoelectric Point: 5.9727
>APO16966.1 IncF plasmid conjugative transfer pilus assembly protein TraC [Proteus mirabilis]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLENP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERVWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAESI
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMNLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHDDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLENP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERVWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAESI
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMNLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHDDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 34217..62553
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_27 | 34217..35452 | + | 1236 | APO16949 | Bipolar DNA helicase HerA | virb4 |
| Locus_28 | 35616..37766 | + | 2151 | APO16950 | Conjugative transfer protein TraI, relaxase | - |
| Locus_29 | 37892..38995 | - | 1104 | APO16951 | putative virulence protein | - |
| Locus_30 | 39129..40964 | + | 1836 | APO16952 | IncF plasmid conjugative transfer protein TraD | virb4 |
| Locus_31 | 40998..41534 | + | 537 | APO16953 | Conjugative transfer protein 234 | - |
| Locus_32 | 41521..42156 | + | 636 | APO16954 | Conjugative transfer protein s043 | tfc7 |
| Locus_33 | 42130..42249 | - | 120 | APO16955 | hypothetical protein | - |
| Locus_34 | 42295..43401 | + | 1107 | APO16956 | Fic family protein | - |
| Locus_35 | 43591..43872 | + | 282 | APO16957 | IncF plasmid conjugative transfer pilus assembly protein TraL | traL |
| Locus_36 | 43869..44495 | + | 627 | APO16958 | IncF plasmid conjugative transfer pilus assembly protein TraE | traE |
| Locus_37 | 44479..45375 | + | 897 | APO16959 | IncF plasmid conjugative transfer pilus assembly protein TraK | traK |
| Locus_38 | 45378..46667 | + | 1290 | APO16960 | IncF plasmid conjugative transfer pilus assembly protein TraB | traB |
| Locus_39 | 46664..47314 | + | 651 | APO16961 | Conjugative transfer protein TraV | traV |
| Locus_40 | 47311..47697 | + | 387 | APO16962 | Conjugative transfer protein TraA | - |
| Locus_41 | 47738..48244 | - | 507 | APO16963 | Acetyltransferase | - |
| Locus_42 | 48235..48501 | - | 267 | APO16964 | hypothetical protein | - |
| Locus_43 | 48870..49562 | + | 693 | APO16965 | Thiol:disulfide involved in conjugative transfer | trbB |
| Locus_44 | 49563..51962 | + | 2400 | APO16966 | IncF plasmid conjugative transfer pilus assembly protein TraC | virb4 |
| Locus_45 | 51955..52302 | + | 348 | APO16967 | Conjugative transfer protein 345 | - |
| Locus_46 | 52286..52798 | + | 513 | APO16968 | Conjugative signal peptidase TrhF | - |
| Locus_47 | 52809..53933 | + | 1125 | APO16969 | IncF plasmid conjugative transfer pilus assembly protein TraW | traW |
| Locus_48 | 53965..54945 | + | 981 | APO16970 | IncF plasmid conjugative transfer pilus assembly protein TraU | traU |
| Locus_49 | 54948..58640 | + | 3693 | APO16971 | IncF plasmid conjugative transfer protein TraN | traN |
| Locus_50 | 59099..60517 | + | 1419 | APO16972 | hypothetical protein | - |
| Locus_51 | 60514..62553 | + | 2040 | APO16973 | Bipolar DNA helicase HerA | virb4 |
| Locus_52 | 62962..63675 | - | 714 | APO16974 | Endonuclease I precursor | - |
| Locus_53 | 64961..65473 | - | 513 | APO16975 | Lipoprotein signal peptidase | - |
| Locus_54 | 65477..65854 | - | 378 | APO16976 | Cobalt-zinc-cadmium resistance protein CzcD | - |
Host bacterium
| ID | 109 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEPmiCHN2410 | GenBank | KX243406 |
| Element size | 93537 bp | Coordinate of oriT [Strand] | 4309..4607 [+] |
| Host bacterium | Proteus mirabilis strain 09MAS2410 sequence | Coordinate of element | 1..93537 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |