Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   200107
Name   oriT_ICEPmiCHN2410 in_silico
Organism   Proteus mirabilis strain 09MAS2410 sequence
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   KX243406 (4309..4607 [+], 299 nt)
oriT length   299 nt
IRs (inverted repeats)      45..50, 52..57  (CAAACG..CGTTTG)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 299 nt

>oriT_ICEPmiCHN2410
GCTCTGTTTGGCGGCGGATGACCTAGTCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGACGATACGTCGTTTTAGGCGTTTTTAAGTGAAATCGGGCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   14260 GenBank   APO16950
Name   traI_-_ICEPmiCHN2410 insolico UniProt ID   _
Length   716 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 716 a.a.        Molecular weight: 79650.73 Da        Isoelectric Point: 5.5209

>APO16950.1 Conjugative transfer protein TraI, relaxase [Proteus mirabilis]
MFKNLFFQAKTLPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLAIKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSGVTLFSKPEWEATQQTQAEPQSRSSEHSDL
PEASSSIEHSNSAESPSSKSSDQDDEIRLASDVNHLQATENAPGDECEKPNNSYDGAIANKVNQHDAEAL
NLSESLAWLPEASSALVVVGEQILIRYPDAVRHWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNAGV
QEQGLLLKGFVSKGLAALIDLSKQDAESVTAIQNKEASPRSSRTETTSAQAKELATRAERKQKPIAPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
RFDEGETVLFTAHAKR

  Protein domains


Predicted by InterproScan.

(39-347)


  Protein structure



No available structure.




T4CP


ID   16506 GenBank   APO16952
Name   traD_-_ICEPmiCHN2410 insolico UniProt ID   _
Length   611 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 611 a.a.        Molecular weight: 67677.58 Da        Isoelectric Point: 6.6443

>APO16952.1 IncF plasmid conjugative transfer protein TraD [Proteus mirabilis]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQMG
LKGKPLAFMALAHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHASYRKNSQMGSTW
IHGIEPKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACI
AAGSPERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTS
QRPDLKTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAAN
TDLEGLLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGL
DSLTDAMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIAT
QTFADFAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTG
NHGERLMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA

  Protein domains


Predicted by InterproScan.

(157-290)

(456-583)

  Protein structure



No available structure.



ID   16507 GenBank   APO16966
Name   traC_-_ICEPmiCHN2410 insolico UniProt ID   _
Length   799 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 799 a.a.        Molecular weight: 90181.69 Da        Isoelectric Point: 5.9727

>APO16966.1 IncF plasmid conjugative transfer pilus assembly protein TraC [Proteus mirabilis]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLENP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERVWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAESI
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMNLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHDDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE

  Protein domains


Predicted by InterproScan.

(11-241)

(436-521)

(568-794)

(253-420)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 34217..62553

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Locus_27 34217..35452 + 1236 APO16949 Bipolar DNA helicase HerA virb4
Locus_28 35616..37766 + 2151 APO16950 Conjugative transfer protein TraI, relaxase -
Locus_29 37892..38995 - 1104 APO16951 putative virulence protein -
Locus_30 39129..40964 + 1836 APO16952 IncF plasmid conjugative transfer protein TraD virb4
Locus_31 40998..41534 + 537 APO16953 Conjugative transfer protein 234 -
Locus_32 41521..42156 + 636 APO16954 Conjugative transfer protein s043 tfc7
Locus_33 42130..42249 - 120 APO16955 hypothetical protein -
Locus_34 42295..43401 + 1107 APO16956 Fic family protein -
Locus_35 43591..43872 + 282 APO16957 IncF plasmid conjugative transfer pilus assembly protein TraL traL
Locus_36 43869..44495 + 627 APO16958 IncF plasmid conjugative transfer pilus assembly protein TraE traE
Locus_37 44479..45375 + 897 APO16959 IncF plasmid conjugative transfer pilus assembly protein TraK traK
Locus_38 45378..46667 + 1290 APO16960 IncF plasmid conjugative transfer pilus assembly protein TraB traB
Locus_39 46664..47314 + 651 APO16961 Conjugative transfer protein TraV traV
Locus_40 47311..47697 + 387 APO16962 Conjugative transfer protein TraA -
Locus_41 47738..48244 - 507 APO16963 Acetyltransferase -
Locus_42 48235..48501 - 267 APO16964 hypothetical protein -
Locus_43 48870..49562 + 693 APO16965 Thiol:disulfide involved in conjugative transfer trbB
Locus_44 49563..51962 + 2400 APO16966 IncF plasmid conjugative transfer pilus assembly protein TraC virb4
Locus_45 51955..52302 + 348 APO16967 Conjugative transfer protein 345 -
Locus_46 52286..52798 + 513 APO16968 Conjugative signal peptidase TrhF -
Locus_47 52809..53933 + 1125 APO16969 IncF plasmid conjugative transfer pilus assembly protein TraW traW
Locus_48 53965..54945 + 981 APO16970 IncF plasmid conjugative transfer pilus assembly protein TraU traU
Locus_49 54948..58640 + 3693 APO16971 IncF plasmid conjugative transfer protein TraN traN
Locus_50 59099..60517 + 1419 APO16972 hypothetical protein -
Locus_51 60514..62553 + 2040 APO16973 Bipolar DNA helicase HerA virb4
Locus_52 62962..63675 - 714 APO16974 Endonuclease I precursor -
Locus_53 64961..65473 - 513 APO16975 Lipoprotein signal peptidase -
Locus_54 65477..65854 - 378 APO16976 Cobalt-zinc-cadmium resistance protein CzcD -


Host bacterium


ID   109 Element type   ICE (Integrative and conjugative element)
Element name   ICEPmiCHN2410 GenBank   KX243406
Element size   93537 bp Coordinate of oriT [Strand]   4309..4607 [+]
Host bacterium   Proteus mirabilis strain 09MAS2410 sequence Coordinate of element   1..93537

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -