Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200106 |
| Name | oriT_ICEPmiCHN2407  |
| Organism | Proteus mirabilis strain 09MAS2407 sequence |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | KX243405 (4309..4607 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 45..50, 52..57 (CAAACG..CGTTTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEPmiCHN2407
GCTCTGTTTGGCGGCGGATGACCTAGTCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGACGATACGTCGTTTTAGGCGTTTTTAAGTGAAATCGGGCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCGGATGACCTAGTCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGACGATACGTCGTTTTAGGCGTTTTTAAGTGAAATCGGGCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14259 | GenBank | APO16855 |
| Name | traI_-_ICEPmiCHN2407 |
UniProt ID | _ |
| Length | 716 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 716 a.a. Molecular weight: 79650.73 Da Isoelectric Point: 5.5209
>APO16855.1 Conjugative transfer protein TraI, relaxase [Proteus mirabilis]
MFKNLFFQAKTLPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLAIKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSGVTLFSKPEWEATQQTQAEPQSRSSEHSDL
PEASSSIEHSNSAESPSSKSSDQDDEIRLASDVNHLQATENAPGDECEKPNNSYDGAIANKVNQHDAEAL
NLSESLAWLPEASSALVVVGEQILIRYPDAVRHWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNAGV
QEQGLLLKGFVSKGLAALIDLSKQDAESVTAIQNKEASPRSSRTETTSAQAKELATRAERKQKPIAPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
RFDEGETVLFTAHAKR
MFKNLFFQAKTLPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLAIKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSGVTLFSKPEWEATQQTQAEPQSRSSEHSDL
PEASSSIEHSNSAESPSSKSSDQDDEIRLASDVNHLQATENAPGDECEKPNNSYDGAIANKVNQHDAEAL
NLSESLAWLPEASSALVVVGEQILIRYPDAVRHWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNAGV
QEQGLLLKGFVSKGLAALIDLSKQDAESVTAIQNKEASPRSSRTETTSAQAKELATRAERKQKPIAPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
RFDEGETVLFTAHAKR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16504 | GenBank | APO16857 |
| Name | traD_-_ICEPmiCHN2407 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 67677.58 Da Isoelectric Point: 6.6443
>APO16857.1 IncF plasmid conjugative transfer protein TraD [Proteus mirabilis]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQMG
LKGKPLAFMALAHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHASYRKNSQMGSTW
IHGIEPKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACI
AAGSPERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTS
QRPDLKTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAAN
TDLEGLLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGL
DSLTDAMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIAT
QTFADFAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTG
NHGERLMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQMG
LKGKPLAFMALAHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHASYRKNSQMGSTW
IHGIEPKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACI
AAGSPERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTS
QRPDLKTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAAN
TDLEGLLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGL
DSLTDAMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIAT
QTFADFAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTG
NHGERLMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16505 | GenBank | APO16871 |
| Name | traC_-_ICEPmiCHN2407 |
UniProt ID | _ |
| Length | 799 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 799 a.a. Molecular weight: 90181.69 Da Isoelectric Point: 5.9727
>APO16871.1 IncF plasmid conjugative transfer pilus assembly protein TraC [Proteus mirabilis]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLENP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERVWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAESI
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMNLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHDDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLENP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERVWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAESI
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMNLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHDDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 34252..62588
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_27 | 34252..35487 | + | 1236 | APO16854 | Bipolar DNA helicase HerA | virb4 |
| Locus_28 | 35651..37801 | + | 2151 | APO16855 | Conjugative transfer protein TraI, relaxase | - |
| Locus_29 | 37927..39030 | - | 1104 | APO16856 | putative virulence protein | - |
| Locus_30 | 39164..40999 | + | 1836 | APO16857 | IncF plasmid conjugative transfer protein TraD | virb4 |
| Locus_31 | 41009..41569 | + | 561 | APO16858 | Conjugative transfer protein 234 | - |
| Locus_32 | 41547..42191 | + | 645 | APO16859 | Conjugative transfer protein s043 | tfc7 |
| Locus_33 | 42165..42284 | - | 120 | APO16860 | hypothetical protein | - |
| Locus_34 | 42330..43436 | + | 1107 | APO16861 | Fic family protein | - |
| Locus_35 | 43626..43907 | + | 282 | APO16862 | IncF plasmid conjugative transfer pilus assembly protein TraL | traL |
| Locus_36 | 43904..44530 | + | 627 | APO16863 | IncF plasmid conjugative transfer pilus assembly protein TraE | traE |
| Locus_37 | 44514..45410 | + | 897 | APO16864 | IncF plasmid conjugative transfer pilus assembly protein TraK | traK |
| Locus_38 | 45413..46702 | + | 1290 | APO16865 | IncF plasmid conjugative transfer pilus assembly protein TraB | traB |
| Locus_39 | 46699..47349 | + | 651 | APO16866 | Conjugative transfer protein TraV | traV |
| Locus_40 | 47346..47732 | + | 387 | APO16867 | Conjugative transfer protein TraA | - |
| Locus_41 | 47773..48279 | - | 507 | APO16868 | Acetyltransferase | - |
| Locus_42 | 48270..48536 | - | 267 | APO16869 | hypothetical protein | - |
| Locus_43 | 48905..49597 | + | 693 | APO16870 | Thiol:disulfide involved in conjugative transfer | trbB |
| Locus_44 | 49598..51997 | + | 2400 | APO16871 | IncF plasmid conjugative transfer pilus assembly protein TraC | virb4 |
| Locus_45 | 51990..52337 | + | 348 | APO16872 | Conjugative transfer protein 345 | - |
| Locus_46 | 52321..52833 | + | 513 | APO16873 | Conjugative signal peptidase TrhF | - |
| Locus_47 | 52844..53968 | + | 1125 | APO16874 | IncF plasmid conjugative transfer pilus assembly protein TraW | traW |
| Locus_48 | 53952..54980 | + | 1029 | APO16875 | IncF plasmid conjugative transfer pilus assembly protein TraU | traU |
| Locus_49 | 54983..58675 | + | 3693 | APO16876 | IncF plasmid conjugative transfer protein TraN | traN |
| Locus_50 | 59134..60552 | + | 1419 | APO16877 | hypothetical protein | - |
| Locus_51 | 60549..62588 | + | 2040 | APO16878 | Bipolar DNA helicase HerA | virb4 |
| Locus_52 | 62997..63710 | - | 714 | APO16879 | Endonuclease I precursor | - |
| Locus_53 | 63713..64429 | - | 717 | APO16880 | Il-IS_2, transposase | - |
| Locus_54 | 64451..64963 | - | 513 | APO16881 | Lipoprotein signal peptidase | - |
| Locus_55 | 64967..65308 | - | 342 | APO16882 | Cobalt-zinc-cadmium resistance protein CzcD | - |
| Locus_56 | 66211..66618 | + | 408 | APO16883 | Cd(II)/Pb(II)-responsive transcriptional regulator | - |
| Locus_57 | 66803..66925 | + | 123 | APO16884 | hypothetical protein | - |
| Locus_58 | 66937..67158 | - | 222 | APO16885 | hypothetical protein | - |
| Locus_59 | 67155..67433 | - | 279 | APO16886 | Resolvase | - |
Host bacterium
| ID | 108 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEPmiCHN2407 | GenBank | KX243405 |
| Element size | 97078 bp | Coordinate of oriT [Strand] | 4309..4607 [+] |
| Host bacterium | Proteus mirabilis strain 09MAS2407 sequence | Coordinate of element | 1..97078 |
Cargo genes
| Drug resistance gene | tet(C) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |