Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200101 |
| Name | oriT_ICEPae690  |
| Organism | Pseudomonas aeruginosa tRNA-Glu and tRNA-Gly genes and integrative |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | KY852375 (78353..78670 [+], 318 nt) |
| oriT length | 318 nt |
| IRs (inverted repeats) | 199..206, 215..222 (TAAAAGGC..GCCTTTTA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 318 nt
>oriT_ICEPae690
CCATGGCCGACCTGTCGTGTCTGCGCCAGCGCAAGAAGACGGTTCCTGCGCCAGTGGTCTGTTGGGCCAGTTCGATGGGCAGCAGGTGGAAGGGGTGCCCGCATGCCTGCTCCAGTACCTGTCGCTGTGCCAGGGCAATCAACTCGTCGCGCATGGCGAAGCACTGGCTGGCCCAGGCCTCGAAGTCCCCTTTACCCTTAAAAGGCTTTAAAAGGCCTTTTAGATAGGCCGCGTGTTCCAGCCGCATGAAGGCACCCTGTTGCAGGCCTCGGAAGTAGCGGGTCGGTGGGTTCGGATCGCTCATGCCGGCTCGTCCTC
CCATGGCCGACCTGTCGTGTCTGCGCCAGCGCAAGAAGACGGTTCCTGCGCCAGTGGTCTGTTGGGCCAGTTCGATGGGCAGCAGGTGGAAGGGGTGCCCGCATGCCTGCTCCAGTACCTGTCGCTGTGCCAGGGCAATCAACTCGTCGCGCATGGCGAAGCACTGGCTGGCCCAGGCCTCGAAGTCCCCTTTACCCTTAAAAGGCTTTAAAAGGCCTTTTAGATAGGCCGCGTGTTCCAGCCGCATGAAGGCACCCTGTTGCAGGCCTCGGAAGTAGCGGGTCGGTGGGTTCGGATCGCTCATGCCGGCTCGTCCTC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14255 | GenBank | ATB17829 |
| Name | TraI_2_-_ICEPae690 |
UniProt ID | _ |
| Length | 612 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 612 a.a. Molecular weight: 67294.28 Da Isoelectric Point: 6.4743
>ATB17829.1 Integrating conjugative element relaxase [Pseudomonas aeruginosa]
MLSLFQRKRPPVAAAPTPSPVTDLPKGLLRPESAASLLATPRRQKLLEHIWQRTSLSHRQFVTLYRTPLE
RYAELVQAFPASEAHHHAYPGGMLDHGLEIVAYSLKLRQSHLLPIGASPEDQAAQSEAWTAAVAYAALLH
DIGKIAVDLHVELADGSTWHPWHGPLHQAYRFRYRDDREYRLHSAATGLLYRQLLDRHVLDWLSDYPALW
APLLYVLAGQYEHAGVLGELVVQADRASVAQELGGDPARAMAAPKHALQRKLLDGLRYLLKEQLKLNQPE
ASDGWLTEDGLWLVSKTVSDKLRAHLLSQGIDGIPANNTAVFNVLQDHGMLQPTSDGKAVWRATVTSTTG
WSHSFTLLRLAPALIWESGERPAPFAGTVEIDATPAENDASMSAPVPTVSVNPAQGGQEPPIWEDDSTTI
VSPPAAQPVPDVMEDLLAMVGLGESAGVGQDAEEFLHPPAPATAATSIPSPVPAPTPGSSATKPSTEQFM
VWLKQGITSRRLIINDAKALVHTVNDTAYLVSPGVFQRYAQEHPEVAALAKQENQQDWQWVQKRFEKLQL
HRKHPNGLNIWTCEVTGPRKSRRLHGYLLEDGSLAFPEIPPNNPYLALTQEG
MLSLFQRKRPPVAAAPTPSPVTDLPKGLLRPESAASLLATPRRQKLLEHIWQRTSLSHRQFVTLYRTPLE
RYAELVQAFPASEAHHHAYPGGMLDHGLEIVAYSLKLRQSHLLPIGASPEDQAAQSEAWTAAVAYAALLH
DIGKIAVDLHVELADGSTWHPWHGPLHQAYRFRYRDDREYRLHSAATGLLYRQLLDRHVLDWLSDYPALW
APLLYVLAGQYEHAGVLGELVVQADRASVAQELGGDPARAMAAPKHALQRKLLDGLRYLLKEQLKLNQPE
ASDGWLTEDGLWLVSKTVSDKLRAHLLSQGIDGIPANNTAVFNVLQDHGMLQPTSDGKAVWRATVTSTTG
WSHSFTLLRLAPALIWESGERPAPFAGTVEIDATPAENDASMSAPVPTVSVNPAQGGQEPPIWEDDSTTI
VSPPAAQPVPDVMEDLLAMVGLGESAGVGQDAEEFLHPPAPATAATSIPSPVPAPTPGSSATKPSTEQFM
VWLKQGITSRRLIINDAKALVHTVNDTAYLVSPGVFQRYAQEHPEVAALAKQENQQDWQWVQKRFEKLQL
HRKHPNGLNIWTCEVTGPRKSRRLHGYLLEDGSLAFPEIPPNNPYLALTQEG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16495 | GenBank | ATB17824 |
| Name | t4cp2_-_ICEPae690 |
UniProt ID | _ |
| Length | 687 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 687 a.a. Molecular weight: 76596.41 Da Isoelectric Point: 6.9863
>ATB17824.1 Conjugative coupling factor TraD, PFGI-1 class [Pseudomonas aeruginosa]
MGVGSALAFGAYGAIRYRDARVILRYRRNIRRLPRYVMTSKDVPVSQQRLFIGRGFLWEQKHTHRLMQTY
RPEFRRYAEPTPAYRLARRLEERLEFAPFPLSRLSALTGWDVPFNPVRPLPPVGGLPRLHGIEPDEVDVS
LPLGERVGHSLVLGTTRVGKTRLAELFVTQDIRRKNAAGEHEVVIVIDPKGDADLLKRMYVEAQRAGREG
EFYIFHLGWPEISARYNAVGRFGRISEVATRIAGQLSGEGNSAAFREFAWRFVNIIARALVELGQRPDYM
LIQRHVINIDALFIEYAQHYFAKTEPKAWEVIVQIEAKLNEKNIPRNMIGREKRVVALEQYLSQARNYDP
VLDGLRSAVRYDKTYFDKIVASLLPLLEKLTSGKIAQLLAPNYSDLTDPRPIFDWMQVIRKRAIVYVGLD
ALSDAEVAAAVGNSMFSDLVSVAGHIYKHGIDDGLPGASAGTRVPINVHADEFNELMGDEFVPLINKGGG
AGLQVTAYTQTLSDIEARIGNRAKAGQVIGNFNNLFMLRVRETATAELLTRQLPKVEVYTTTIVSGATDS
SDIRGATDFTSNTQDRISMSSVPMIEPSHVVGLPKGQCFALLQGGQLWKVRMPLPTPDPDEVMPADLQQL
AGYMRQSYSEATQWWDFTSSPALQDAALPDDLLDDAAPAEPAAVATDTDHSTDEAAP
MGVGSALAFGAYGAIRYRDARVILRYRRNIRRLPRYVMTSKDVPVSQQRLFIGRGFLWEQKHTHRLMQTY
RPEFRRYAEPTPAYRLARRLEERLEFAPFPLSRLSALTGWDVPFNPVRPLPPVGGLPRLHGIEPDEVDVS
LPLGERVGHSLVLGTTRVGKTRLAELFVTQDIRRKNAAGEHEVVIVIDPKGDADLLKRMYVEAQRAGREG
EFYIFHLGWPEISARYNAVGRFGRISEVATRIAGQLSGEGNSAAFREFAWRFVNIIARALVELGQRPDYM
LIQRHVINIDALFIEYAQHYFAKTEPKAWEVIVQIEAKLNEKNIPRNMIGREKRVVALEQYLSQARNYDP
VLDGLRSAVRYDKTYFDKIVASLLPLLEKLTSGKIAQLLAPNYSDLTDPRPIFDWMQVIRKRAIVYVGLD
ALSDAEVAAAVGNSMFSDLVSVAGHIYKHGIDDGLPGASAGTRVPINVHADEFNELMGDEFVPLINKGGG
AGLQVTAYTQTLSDIEARIGNRAKAGQVIGNFNNLFMLRVRETATAELLTRQLPKVEVYTTTIVSGATDS
SDIRGATDFTSNTQDRISMSSVPMIEPSHVVGLPKGQCFALLQGGQLWKVRMPLPTPDPDEVMPADLQQL
AGYMRQSYSEATQWWDFTSSPALQDAALPDDLLDDAAPAEPAAVATDTDHSTDEAAP
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 39217..59332
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_32 | 35328..37166 | - | 1839 | ATB17829 | Integrating conjugative element relaxase | - |
| Locus_33 | 37484..38110 | + | 627 | ATB17870 | hypothetical protein | - |
| Locus_34 | 38123..38755 | + | 633 | ATB17869 | hypothetical protein | - |
| Locus_35 | 38840..39205 | + | 366 | ATB17893 | hypothetical protein | - |
| Locus_36 | 39217..40737 | - | 1521 | ATB17832 | TraG-like protein | tfc19 |
| Locus_37 | 40753..41109 | - | 357 | ATB17895 | hypothetical protein | tfc18 |
| Locus_38 | 41106..42500 | - | 1395 | ATB17834 | Integrating conjugative element protein | tfc22 |
| Locus_39 | 42510..43457 | - | 948 | ATB17845 | TraU protein | tfc23 |
| Locus_40 | 43454..43900 | - | 447 | ATB17882 | hypothetical protein | tfc24 |
| Locus_41 | 44062..44556 | - | 495 | ATB17880 | DNA repair protein RadC | - |
| Locus_42 | 44732..45496 | - | 765 | ATB17858 | Disulfide bond formation protein DsbA | - |
| Locus_43 | 45510..48377 | - | 2868 | ATB17821 | Conjugative transfer ATPase | virb4 |
| Locus_44 | 48377..48817 | - | 441 | ATB17884 | Conjugal transfer protein | tfc15 |
| Locus_45 | 48798..50216 | - | 1419 | ATB17833 | Integrating conjugative element protein | tfc14 |
| Locus_46 | 50206..51111 | - | 906 | ATB17848 | Integrating conjugative element protein | tfc13 |
| Locus_47 | 51108..51797 | - | 690 | ATB17865 | Integrating conjugative element protein | tfc12 |
| Locus_48 | 51794..52192 | - | 399 | ATB17888 | Conjugative transfer region protein | tfc11 |
| Locus_49 | 52204..52563 | - | 360 | ATB17894 | Integrating conjugative element membrane protein | tfc10 |
| Locus_50 | 52580..52813 | - | 234 | ATB17907 | Integrating conjugative element protein | tfc9 |
| Locus_51 | 52810..53193 | - | 384 | ATB17892 | Integrative conjugative element protein | tfc8 |
| Locus_52 | 53292..54041 | - | 750 | ATB17860 | Integrating conjugative element membrane protein | tfc7 |
| Locus_53 | 54038..56101 | - | 2064 | ATB17824 | Conjugative coupling factor TraD, PFGI-1 class | - |
| Locus_54 | 56232..56771 | - | 540 | ATB17878 | Integrating conjugative element protein | tfc5 |
| Locus_55 | 56768..57373 | - | 606 | ATB17872 | Lytic transglycosylase | virB1 |
| Locus_56 | 57355..58080 | - | 726 | ATB17863 | Integrating conjugative element protein | tfc3 |
| Locus_57 | 58095..58736 | - | 642 | ATB17868 | Chromosome segregation protein SMC | - |
| Locus_58 | 58733..59332 | - | 600 | ATB17873 | Integrating conjugative element protein pill, pfgi-1 | tfc2 |
| Locus_59 | 59480..59686 | - | 207 | ATB17910 | hypothetical protein | - |
| Locus_60 | 59802..62081 | - | 2280 | ATB17823 | DEAD/DEAH box helicase | - |
| Locus_61 | 62217..62522 | - | 306 | ATB17900 | hypothetical protein | - |
| Locus_62 | 62613..62933 | - | 321 | ATB17899 | hypothetical protein | - |
| Locus_63 | 62985..64094 | - | 1110 | ATB17840 | SAM-dependent methyltransferase | - |
Host bacterium
| ID | 103 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEPae690 | GenBank | KY852375 |
| Element size | 86600 bp | Coordinate of oriT [Strand] | 78353..78670 [+] |
| Host bacterium | Pseudomonas aeruginosa tRNA-Glu and tRNA-Gly genes and integrative | Coordinate of element | 315..86517 |
Cargo genes
| Drug resistance gene | blaGES-5, aac(6')-Il, qacE, sul1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA7 |