Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200094
Name   oriT_TnGBS2 experimental
Organism   Streptococcus agalactiae NEM316
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_004368 (1169495..1169527 [-], 33 nt)
oriT length   33 nt
IRs (inverted repeats)      1..10, 11..20  (GCATGTGTCC..GGACACGTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   

  oriT sequence  


Download         Length: 33 nt

>oriT_TnGBS2
GCATGTGTCCGGACACGTGCTACGCTTGCCAGA

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Romain Guérillot et al. (2013) Modular evolution of TnGBSs, a new family of integrative and conjugative elements associating insertion sequence transposition, plasmid replication, and conjugation for their spreading. Journal of bacteriology. 195(9):1979-90. [PMID:23435978]


Relaxase

ID   1098 GenBank   WP_000261836
Name   gbs1121_TnGBS2 insolico UniProt ID   Q8E5B1
Length   543 a.a. PDB ID   
Note   

  Relaxase protein sequence


Download         Length: 543 a.a.        Molecular weight: 63241.72 Da        Isoelectric Point: 6.9556

>WP_000261836.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Streptococcus]
MVVTKVIQIKSSKNLKRAINYIARDDATLIKGNGNLADEDYSYEVVNGQVMKRLVSGHDLTDISDTDMIY
EDFMLLKQSVDALYDNDTLSDLKNPNRVLAHHVIQSFSPNDELTPEEVNEIGRKTALELTGGDYQFVVAT
HQDKGHLHNHIIFSTTNQVTLKKFRWQKSTKKSLEHISDKHAELYGAKILEPKLRNSYTDYSAWRRKNNF
RYEIKERLDFLLKHSLDKDDFFQKAKALNLHIDTSGKYVTYKLIDSPQERPVRDRTLSKKGKYYLDKMVE
RFAINEVVYNLDHIKEKYDEEQAKKAEDFEMKVRIEPWQIKQLTNQSIHVSIIFGLDRQGTVAIPARMLD
QNEDGSFTAYLKKNDFFYFLNADHSEQNRFVKGTTLIKQLSAQNGEVILTKNKHVAELNRLVDEFNFLAA
NQVTDSTQFMQLKETFLEQLVETDKTLEQLDDKMTYLNKVLGALMDYQNGIIPSEVTMTILDKAKVDKDG
DTKSLRKEIKELQIERETLHKVRDQIVNDYDFAIEMTNRYDKSNKESKRRSML

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(13-270)


  Protein structure


Source ID Structure
AlphaFold DB Q8E5B1

  Reference


[1] Romain Guérillot et al. (2013) Modular evolution of TnGBSs, a new family of integrative and conjugative elements associating insertion sequence transposition, plasmid replication, and conjugation for their spreading. Journal of bacteriology. 195(9):1979-90. [PMID:23435978]


Host bacterium

ID   101 Element type   ICE (Integrative and conjugative element)
Element name   TnGBS2 GenBank   NC_004368
Element size   2211485 bp Coordinate of oriT [Strand]   1169495..1169527 [-]
Host bacterium   Streptococcus agalactiae NEM316 Coordinate of element   1163887..1197331

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA7, AcrIIA21