Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200084 |
| Name | oriT_cLV25  |
| Organism | Bacteroides fragilis |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | AY053505 (6575..6927 [+], 353 nt) |
| oriT length | 353 nt |
| IRs (inverted repeats) | IR1: 37..45, 51..59 (CTTTGCTGT..AAAGCAAGG) IR2: 62..69, 72..79 (GTTTCGGT..ACCGAAAC) |
| Location of nic site | 88..89 |
| Conserved sequence flanking the nic site |
CATAACCTTG|CT |
| Note |
oriT sequence
Download Length: 353 nt
>oriT_cLV25
GGTATCTTAAAAATTGCGACCAACGGGAGAAATTTTCTTTGCTGTCAAGCAAAGCAAGGTAGTTTCGGTTTACCGAAACATAACCTTGCTCTCCAAATCAAACCACCTGCCGCTGGTAATCCTGCCGGACTAAATGCCCCTACCCCTTTTCTGTCTTGGGGTGGTAGGTTGTCCGGTTTCCTGCTTTATCTCCTGCGCTTGGCTGGTGCTGTTCTTCTGTTCCTGCTTCCTGCCGCACAGGTAATCGTTCAAGTCCGAATACCCCCTGTAATTGTCCGAGAAGTCACGCACACGGTAGGAGTATTCCAATTCGATAGCCCGTGTCGCTACATATCCTGCCTTGTCATTGTCAA
GGTATCTTAAAAATTGCGACCAACGGGAGAAATTTTCTTTGCTGTCAAGCAAAGCAAGGTAGTTTCGGTTTACCGAAACATAACCTTGCTCTCCAAATCAAACCACCTGCCGCTGGTAATCCTGCCGGACTAAATGCCCCTACCCCTTTTCTGTCTTGGGGTGGTAGGTTGTCCGGTTTCCTGCTTTATCTCCTGCGCTTGGCTGGTGCTGTTCTTCTGTTCCTGCTTCCTGCCGCACAGGTAATCGTTCAAGTCCGAATACCCCCTGTAATTGTCCGAGAAGTCACGCACACGGTAGGAGTATTCCAATTCGATAGCCCGTGTCGCTACATATCCTGCCTTGTCATTGTCAA
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Kathleen A Bass et al. (2002) Isolation and characterization of cLV25, a Bacteroides fragilis chromosomal transfer factor resembling multiple Bacteroides sp. mobilizable transposons. Journal of bacteriology. 184(7):1895-904. [PMID:11889096]
Relaxase
| ID | 1093 | GenBank | AAL29920 |
| Name | BmgA_cLV25 |
UniProt ID | Q8RM71 |
| Length | 308 a.a. | PDB ID | |
| Note | |||
Relaxase protein sequence
Download Length: 308 a.a. Molecular weight: 34741.80 Da Isoelectric Point: 10.1804
>AAL29920.1 mobilization protein BmgA [Bacteroides fragilis]
MIGKIKKGSGFKGCVNYVLGKEQAALLHADGVLTESRGDIIRSFCMQTGMNPDLKKPVGHIALSYSAVDA
PKLTDEKMIQLAQEYMREMKITDTQYIIVRHQDREHPHVHIVFNRIDNNGKTISDRNDMYRNEQVCKKLK
AKHGLYFAGGKEQVKQHRLKEPDKSKYEIYHAVKNEIGKSRNWQQLQERLAEKGITIRFKYTGQTSEIQG
VSFSKGEYTFKGSEIDRSFSFSKLDKCFGDVGQTTAGSNKQAVTAPIQKSVSAPDKTDNSFITGLGGLFS
GLSAPADEMPDDSFLRKKKRKKKKQLKL
MIGKIKKGSGFKGCVNYVLGKEQAALLHADGVLTESRGDIIRSFCMQTGMNPDLKKPVGHIALSYSAVDA
PKLTDEKMIQLAQEYMREMKITDTQYIIVRHQDREHPHVHIVFNRIDNNGKTISDRNDMYRNEQVCKKLK
AKHGLYFAGGKEQVKQHRLKEPDKSKYEIYHAVKNEIGKSRNWQQLQERLAEKGITIRFKYTGQTSEIQG
VSFSKGEYTFKGSEIDRSFSFSKLDKCFGDVGQTTAGSNKQAVTAPIQKSVSAPDKTDNSFITGLGGLFS
GLSAPADEMPDDSFLRKKKRKKKKQLKL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q8RM71 |
Reference
[1] Kathleen A Bass et al. (2002) Isolation and characterization of cLV25, a Bacteroides fragilis chromosomal transfer factor resembling multiple Bacteroides sp. mobilizable transposons. Journal of bacteriology. 184(7):1895-904. [PMID:11889096]
Host bacterium
| ID | 92 | Element type | IME (Integrative mobilizable element) |
| Element name | cLV25 | GenBank | AY053505 |
| Element size | 15343 bp | Coordinate of oriT [Strand] | 6575..6927 [+] |
| Host bacterium | Bacteroides fragilis |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |