Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200080
Name   oriT_GGI experimental
Organism   Neisseria gonorrhoeae strain MS11
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   AY803022 (6050..6099 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      13..25, 32..44  (CAAAGGCCTGCAT..ATGCAGGCCTTTG)
Location of nic site      46..47
Conserved sequence flanking the
  nic site    		  TTGCCT|A
Note   The MOBH family relaxase TraI_GGI cleaves oriT_GGI in a site-specific and Mn2+-dependent manner [PMID:21255116, PMID:22016851].

  oriT sequence  


Download         Length: 50 nt

>oriT_GGI
TTAAAGGTAAAACAAAGGCCTGCATGTAAAAATGCAGGCCTTTGCCTATT

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Jan-Hendrik Heilers et al. (2019) DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island. Nucleic acids research. 47(15):8136-8153. [PMID:31276596]


Relaxase

ID   1069 GenBank   WP_003693100
Name   TraI_GGI experimental UniProt ID   D6H7W1
Length   850 a.a. PDB ID   
Note   The MOBH family relaxase TraI_GGI cleaves oriT_GGI in a site-specific and Mn2+-dependent manner [PMID:21255116, PMID:22016851].

  Relaxase protein sequence


Download         Length: 850 a.a.        Molecular weight: 94671.82 Da        Isoelectric Point: 5.9293

>WP_003693100.1 MobH family relaxase [Neisseria gonorrhoeae]
MKTSLLTIASSLMLVSGSMLYLINTRKSTDSVSHNDEVAHINQNIVNDRFRILNAHELIQVLDLSPQISG
IKMNLGLSDENWSKDALPFLEKYIAFVQRLPASESHHHAGDGGLVRHTLDVAALALVASTSQSWPPNAKT
EEIAKKTAVWRYGIMCAALLHDVGKTVTGFQVELFDSAISLEKLLWLPDTGSMAESGKLYYRVEFPDAKS
AYSTHAEIAWTFFQALVPSHVRQWLATTDPNLMITLRNYLSGKKDGSPLEQLIKNADMTSVSRDLRSGSR
QRFSTAKRKPFIETIMETLKEMLSDRGVHFSIATTAGGDLFRKGDRIYLMSKNVPDYIRQYLRKNQHPAA
GSFPADNQRIFDTLFEYRAVIPPENDPHRAINHIEVEFTRMDGEKVRNIFSVLCFNAKTLYPDGDYPTEF
LGNLSEVSAKKEIPDVVVSEDKVSTQIQGAEDNPVTMTKDKMDFLSIQPVTAEQPPLPEKNQTETANSAF
DNSMNTAAPAENKSSESEKAKEPGYGTIDNLIENFNLIEADSEQTESTKSEAETAENAVNLVQENIRVEV
KTETVDKPRAEVKETETDKAPKTIKSGTKASRKKLAGLFADNRKPIGKTAEPDAQIKTTETVTHQIDSEA
VSLPESESAQNGMRELERLRLREPTSSPRPVQVMDDSEGLDSLASGVKNMAELEAIVSERNLKKQSETES
TEIVEDSVKAKHMEARDRGMQFLRWLADGLGDGSIAVNRSGATVHFIEQGMLLVTPAVFRDYAGGVFNKS
DTESLGPLTQKGFEALRLHARTKKSSLHRVLTTNGSNRRLFYCYLIPEHNIKHIIQPGSRPQNNTDIKLD
ESDLLVMEQK

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(60-372)

Putative conjugal transfer nickase/helicase TraI C-term (TraI_2_C)

(718-821)


  Protein structure


Source ID Structure
AlphaFold DB D6H7W1

  Reference


[1] Jan-Hendrik Heilers et al. (2019) DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island. Nucleic acids research. 47(15):8136-8153. [PMID:31276596]


T4CP

ID   647 GenBank   AAW83117
Name   ParA_GGI experimental UniProt ID   Q5EP68
Length   295 a.a. PDB ID   _
Note   

  T4CP protein sequence


Download         Length: 295 a.a.        Molecular weight: 32561.85 Da        Isoelectric Point: 8.0343

>AAW83117.1 ParA [Neisseria gonorrhoeae]
MSAPVILSAAATKGGVGKTTLIANVSAVLADIGLRVLMIDCDVQPSLSKYYPISHRAPNGIVELLLGENT
EEIIRSTISNTVFPNLDIVISNNISAFVETEVTSRPDRAFLLKSKLHSQYIQNNYDVVLIDTQGAVGPLL
DTAAFAATCLLCPIMPEVLSAREFLTGTQELLKRLQKGEAMRIPMPQLRAIIYAQDRTNDAKFIASSIKD
FFSNTLDDRRKLLSVAVPAAKAYKEATTLRVPVHCHERKQLGKMLPAYEIMHRIVYEIFPGIEQKNLRGS
CFNDMASLLQKEVQS

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_31)

(7-177)

  Protein structure


Source ID Structure
AlphaFold DB Q5EP68

  Reference


[1] Melanie M Callaghan et al. (2021) Expression, Localization, and Protein Interactions of the Partitioning Proteins in the Gonococcal Type IV Secretion System. Frontiers in microbiology. 12:784483. [PMID:34975804]

ID   648 GenBank   AAW83116
Name   ParB_GGI experimental UniProt ID   Q5EP69
Length   496 a.a. PDB ID   _
Note   

  T4CP protein sequence


Download         Length: 496 a.a.        Molecular weight: 56519.41 Da        Isoelectric Point: 4.7899

>AAW83116.1 ParB [Neisseria gonorrhoeae]
MNLDQNKATLSLHIGTPAQTNMSFGQEDTPSRGMLMSLPISEIDFFDKNPRRLHDVSSYAAIKESIRSSG
IQQPVHVTQRPGKSKYVLAQGGNTRLKILCELLEETGDNRFRMMPCIYIPYSDETTLQVAHLVENELRAE
MCFWDKACKYAEIRDIFQDESDSGKLSLRELEVMFSDKGLTVSHKTLGLFFFASDNLQELQEYAFKLSHA
KVEEIKKLHSTLFSISKSIQLDDSFQYLWSDVLEQWHNQYSATELDVHELYQFVLDRFKFEYGSDAFSET
ENSSDKDKIETAAKQDVNLHQKDSDMEFVPAKVNGTKADAEDEPKEAEPITLEQTSKKLFSAVRKLLAKV
SLSDCFKIHKDFPYGWYVEYPAFEHISASSAGNAIYAIDVLHEEAGNVFTFLWKLSGIDTFMFDVGDSSN
PLLTLKSDSKLRIAYEDPDKYDEYTNCGIGNKENIIASLLNWQTNTSHEFHANIVEILSLSTQYNNLRKT
GAKSEE

  Protein domains


Predicted by InterproScan.

ParB/Sulfiredoxin domain (ParBc)

(38-134)

  Protein structure


Source ID Structure
AlphaFold DB Q5EP69

  Reference


[1] Melanie M Callaghan et al. (2021) Expression, Localization, and Protein Interactions of the Partitioning Proteins in the Gonococcal Type IV Secretion System. Frontiers in microbiology. 12:784483. [PMID:34975804]


Host bacterium

ID   88 Element type   ICE (Integrative and conjugative element)
Element name   GGI GenBank   AY803022
Element size   57358 bp Coordinate of oriT [Strand]   6050..6099 [-]
Host bacterium   Neisseria gonorrhoeae strain MS11

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIB