Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200054 |
| Name | oriT_Tn916(RST11)  |
| Organism | Staphylococcus rostri |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | FN550102 (2441..2655 [+], 215 nt) |
| oriT length | 215 nt |
| IRs (inverted repeats) | 61..76, 80..95 (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) 123..134, 139..150 (GAAAATCCTTTG..CAAGGGATTTAC) |
| Location of nic site | 135..136 |
| Conserved sequence flanking the nic site |
TGG|T |
| Note | blastn alignment with the oriT_Tn916 |
oriT sequence
Download Length: 215 nt
>oriT_Tn916(RST11)
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 268 | GenBank | CBE66529 |
| Name | ORF20_Tn916(RST11) |
UniProt ID | C8ZL35 |
| Length | 329 a.a. | PDB ID | |
| Note | putative transcriptional regulator | ||
Relaxase protein sequence
Download Length: 329 a.a. Molecular weight: 39099.72 Da Isoelectric Point: 7.0754
>CBE66529.1 putative transcriptional regulator [Staphylococcus rostri]
MLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGC
RQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGE
LVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLVY
DNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAP
TLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
MLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGC
RQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGE
LVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLVY
DNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAP
TLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | C8ZL35 |
T4CP
| ID | 268 | GenBank | CBE66528 |
| Name | ORF21_Tn916(RST11) |
UniProt ID | C8ZL34 |
| Length | 461 a.a. | PDB ID | _ |
| Note | _ | ||
T4CP protein sequence
Download Length: 461 a.a. Molecular weight: 53370.27 Da Isoelectric Point: 9.0687
>CBE66528.1 hypothetical protein [Staphylococcus rostri]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | C8ZL34 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 336..11746
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_0 | 194..313 | + | 120 | CBE66525 | hypothetical protein | - |
| Locus_1 | 336..650 | + | 315 | CBE66526 | conjugative transposon protein | orf23 |
| Locus_2 | 666..1052 | + | 387 | CBE66527 | conjugative transposon protein | orf23 |
| Locus_3 | 1081..2466 | + | 1386 | CBE66528 | hypothetical protein | virb4 |
| Locus_4 | 2860..3849 | + | 990 | CBE66529 | putative transcriptional regulator | - |
| Locus_5 | 3892..4113 | + | 222 | CBE66530 | conjugative transposon protein | orf19 |
| Locus_6 | 4230..4727 | + | 498 | CBE66531 | conjugative transposon protein | - |
| Locus_7 | 4702..5208 | + | 507 | CBE66532 | conjugative transposon protein | orf17a |
| Locus_8 | 5192..7639 | + | 2448 | CBE66533 | putative regulatory protein | virb4 |
| Locus_9 | 7642..9819 | + | 2178 | CBE66534 | putative membrane protein | orf15 |
| Locus_10 | 9816..10817 | + | 1002 | CBE66535 | putative membrane protein | orf14 |
| Locus_11 | 10814..11746 | + | 933 | CBE66536 | putative membrane protein | orf13 |
| Locus_12 | 12021..12107 | + | 87 | CBE66537 | tetM leader peptide | - |
| Locus_13 | 12108..14042 | + | 1935 | CBE66538 | tetracycline resistance protein | - |
| Locus_14 | 14140..14328 | + | 189 | CBE66539 | putative transcriptional regulator | - |
| Locus_15 | 14388..14741 | + | 354 | Protein_15 | - | - |
| Locus_16 | 14946..15017 | + | 72 | CBE66541 | putative ATP-binding protein | - |
| Locus_17 | 15195..15668 | + | 474 | CBE66542 | putative regulatory protein | - |
| Locus_18 | 15665..15895 | + | 231 | CBE66543 | putative ATP-binding protein | - |
| Locus_19 | 16121..16372 | - | 252 | CBE66544 | excisionase | - |
| Locus_20 | 16356..16559 | + | 204 | CBE66545 | excisionase | - |
Host bacterium
| ID | 59 | Element type | Transposon |
| Element name | Tn916(RST11) | GenBank | FN550102 |
| Element size | 18032 bp | Coordinate of oriT [Strand] | 2441..2655 [+] |
| Host bacterium | Staphylococcus rostri |
Cargo genes
| Drug resistance gene | tet(M) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |