Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200053 |
| Name | oriT_ICESpnH19A6-1  |
| Organism | Streptococcus pneumoniae Hungary19A-6 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | CP000936 (1320264..1320478 [-], 215 nt) |
| oriT length | 215 nt |
| IRs (inverted repeats) | 61..76, 80..95 (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) 123..134, 139..150 (GAAAATCCTTTG..CAAGGGATTTAC) |
| Location of nic site | 135..136 |
| Conserved sequence flanking the nic site |
TGG|T |
| Note | blastn alignment with the oriT_Tn916 |
oriT sequence
Download Length: 215 nt
>oriT_ICESpnH19A6-1
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 267 | GenBank | ACA37101 |
| Name | Relaxase_ICESpnH19A6-1 |
UniProt ID | B1IC95 |
| Length | 409 a.a. | PDB ID | |
| Note | transcriptional regulator, Cro/CI family | ||
Relaxase protein sequence
Download Length: 409 a.a. Molecular weight: 48250.06 Da Isoelectric Point: 6.5921
>ACA37101.1 transcriptional regulator, Cro/CI family [Streptococcus pneumoniae Hungary19A-6]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKLLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKKRISVNHTS
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKLLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKKRISVNHTS
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B1IC95 |
T4CP
| ID | 267 | GenBank | ACA36356 |
| Name | T4CP_ICESpnH19A6-1 |
UniProt ID | B1IC96 |
| Length | 461 a.a. | PDB ID | _ |
| Note | ftsk/spoiiie family protein | ||
T4CP protein sequence
Download Length: 461 a.a. Molecular weight: 53370.27 Da Isoelectric Point: 9.0687
>ACA36356.1 ftsk/spoiiie family protein [Streptococcus pneumoniae Hungary19A-6]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B1IC96 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1308321..1322583
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SPH_1403 | 1303508..1303711 | - | 204 | ACA37419 | conserved domain protein | - |
| SPH_1404 | 1303695..1303946 | + | 252 | ACA35758 | conserved hypothetical protein | - |
| SPH_1405 | 1304172..1304402 | - | 231 | ACA36408 | conserved hypothetical protein | - |
| SPH_1406 | 1304399..1304821 | - | 423 | ACA35641 | sigma-70, region 4 family | - |
| SPH_1407 | 1305050..1305196 | - | 147 | ACA36714 | conserved hypothetical protein | - |
| SPH_1408 | 1305326..1305679 | + | 354 | ACA37224 | transcriptional regulator, putative | - |
| SPH_1409 | 1306025..1307944 | - | 1920 | ACA37605 | tetracycline resistance protein | - |
| SPH_1410 | 1307960..1308076 | - | 117 | ACA37321 | Tetracycline resistance determinant leader peptide | - |
| SPH_1411 | 1308321..1309238 | - | 918 | ACA37003 | conjugative transposon protein | orf13 |
| SPH_1412 | 1309253..1310254 | - | 1002 | ACA36364 | NLP/P60 family protein | orf14 |
| SPH_1413 | 1310251..1312428 | - | 2178 | ACA35787 | conjugative transposon membrane protein | orf15 |
| SPH_1414 | 1312431..1314878 | - | 2448 | ACA37348 | conjugative transposon protein | virb4 |
| SPH_1415 | 1314862..1315368 | - | 507 | ACA36762 | conjugative transposon membrane protein | orf17a |
| SPH_1416 | 1315343..1315840 | - | 498 | ACA37337 | conjugative transposon protein | - |
| SPH_1417 | 1315957..1316178 | - | 222 | ACA36148 | conserved domain protein | orf19 |
| SPH_1418 | 1316358..1317605 | + | 1248 | ACA37581 | transposase | - |
| SPH_1419 | 1317726..1317917 | - | 192 | ACA36129 | conserved hypothetical protein | - |
| SPH_1420 | 1317862..1318599 | - | 738 | ACA36985 | rRNA adenine N-6-methyltransferase (Macrolide-lincosamide-streptogramin B resistance protein) | - |
| SPH_1422 | 1319046..1320275 | - | 1230 | ACA37101 | transcriptional regulator, Cro/CI family | - |
| SPH_1423 | 1320453..1321838 | - | 1386 | ACA36356 | ftsk/spoiiie family protein | virb4 |
| SPH_1424 | 1321867..1322250 | - | 384 | ACA35852 | conjugative transposon protein | orf23 |
| SPH_1425 | 1322269..1322583 | - | 315 | ACA37134 | conjugative transposon protein | orf23 |
| SPH_1426 | 1322606..1322725 | - | 120 | ACA36774 | conserved hypothetical protein | - |
| SPH_1427 | 1323215..1324498 | + | 1284 | ACA36826 | uracil permease | - |
| SPH_1428 | 1324594..1326165 | - | 1572 | ACA35896 | signal recognition particle protein | - |
| SPH_1429 | 1326177..1326509 | - | 333 | ACA37645 | putative helix-turn-helix protein | - |
| SPH_1430 | 1326600..1326977 | - | 378 | ACA35656 | conserved hypothetical protein | - |
Host bacterium
| ID | 58 | Element type | Transposon |
| Element name | ICESpnH19A6-1 | GenBank | CP000936 |
| Element size | 2245615 bp | Coordinate of oriT [Strand] | 1320264..1320478 [-] |
| Host bacterium | Streptococcus pneumoniae Hungary19A-6 | Coordinate of element | 1302035..1322918 |
Cargo genes
| Drug resistance gene | tet(M), erm(B) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |