Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200052
Name   oriT_Tn6079 in_silico
Organism   Uncultured bacterium MID12
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   GU951538 (7612..7763 [+], 152 nt)
oriT length   152 nt
IRs (inverted repeats)      3..7, 19..23  (CCCCT..AGGGG)
  60..71, 76..87  ( GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site      72..73nt
Conserved sequence flanking the
  nic site    		  TGG|T
Note   blastn alignment with the oriT_Tn916

  oriT sequence  


Download         Length: 152 nt

>oriT_Tn6079
AACCCCTCGGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   266 GenBank   ADH43079
Name   Relaxase_Tn6079 insolico UniProt ID   D7PTR9
Length   329 a.a. PDB ID   
Note   Tn916 orf20 protein

  Relaxase protein sequence


Download         Length: 329 a.a.        Molecular weight: 39099.72 Da        Isoelectric Point: 7.0754

>ADH43079.1 Tn916 orf20 protein [uncultured bacterium MID12]
MLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGC
RQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGE
LVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLVY
DNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAP
TLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK

  Protein domains


Predicted by InterproScan.

Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N)

(2-89)

Replication initiation factor (Rep_trans)

(97-301)


  Protein structure


Source ID Structure
AlphaFold DB D7PTR9


T4CP

ID   266 GenBank   ADH43077
Name   T4CP_Tn6079 insolico UniProt ID   D7PTR7
Length   463 a.a. PDB ID   _
Note   Tn916-like orf21 protein

  T4CP protein sequence


Download         Length: 463 a.a.        Molecular weight: 53479.10 Da        Isoelectric Point: 7.0591

>ADH43077.1 Tn916-like orf21 protein [uncultured bacterium MID12]
MRMIWNKGHRIRASDKHLVYHFSIGMLLFIFVTVLLLLNMKQLMCTDWEHFSLLENGLTLSPYNFITIGI
ATGVCALVAFGYYRFCYDGFKKLLHRQKLARMILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQ
MEKGLLHIRCEITLGKYQDQLLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGC
LKLMKNLVWEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNSDLADLGTVMPNVYHTKE
EMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEMLGTKESVSLLSQLKKIV
MLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISELGYGMLFGSDVKKQFFQKRIKGRGYCDVG
TSVISEFYTPLVPKGHDFLQTIGRLAQERQAMPEQQGKEDVIE

  Protein domains


Predicted by InterproScan.

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(217-298)

  Protein structure


Source ID Structure
AlphaFold DB D7PTR7


Host bacterium

ID   57 Element type   Transposon
Element name   Tn6079 GenBank   GU951538
Element size   45360 bp Coordinate of oriT [Strand]   7612..7763 [+]
Host bacterium   Uncultured bacterium MID12 Coordinate of element   462..28872

Cargo genes

Drug resistance gene   tetracycline and erythromycin resistanc
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -