Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200047
• Name: oriT_ICECdiM120-1
• Organism: Clostridioides difficile M120
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_017174 (2186896..2187110 [-], 215 nt)
• oriT length: 215 nt
• IRs (inverted repeats): 61..76, 80..95 (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) 123..134, 139..150 (GAAAATCCTTTG..CAAGGGATTTAC)
• Location of nic site: 135..136
• Conserved sequence flanking the nic site: TGG|T
• Note: blastn alignment with the oriT_Tn916

oriT sequence

• Length: 215 nt

>oriT_ICECdiM120-1
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_ICECdiM120-1

• ID: 261
• GenBank: WP_000398284
• Name: Relaxase_ICECdiM120-1
• UniProt ID: _
• Length: 401 a.a.
• Note: replication initiation protein

Relaxase protein sequence

• Length: 401 a.a.
• Molecular weight: 47398.10 Da
• Isoelectric Point: 6.5091

>WP_000398284.1 MULTISPECIES: MobT family relaxase [Bacteria]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK

Protein domains

Predicted by InterproScan.

• Helix-turn-helix (HTH_3) (15-53)

• Replication initiation factor (Rep_trans) (169-373)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (73-161)

Protein structure

No available structure.

T4CP

T4CP_ICECdiM120-1

• ID: 261
• GenBank: WP_000813488
• Name: T4CP_ICECdiM120-1
• UniProt ID: _
• Length: 461 a.a.
• PDB ID: _
• Note: cell division protein FtsK

T4CP protein sequence

• Length: 461 a.a.
• Molecular weight: 53370.27 Da
• Isoelectric Point: 9.0687

>WP_000813488.1 MULTISPECIES: FtsK/SpoIIIE domain-containing protein [Bacteria]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (217-301)

Protein structure

No available structure.

Host bacterium

• ID: 52
• Element type: Transposon
• Element name: ICECdiM120-1
• GenBank: NC_017174
• Element size: 4047729 bp
• Coordinate of oriT [Strand]: 2186896..2187110 [-]
• Host bacterium: Clostridioides difficile M120
• Coordinate of element: 2171649..2189550

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -