Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200045 |
| Name | oriT_ICESpnTw19F14-1  |
| Organism | Streptococcus pneumoniae Taiwan19F-14 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | CP000921 (1787601..1787815 [+], 215 nt) |
| oriT length | 215 nt |
| IRs (inverted repeats) | 61..76, 80..95 (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) 123..134, 139..150 (GAAAATCCTTTG..CAAGGGATTTAC) |
| Location of nic site | 135..136 |
| Conserved sequence flanking the nic site |
TGG|T |
| Note | blastn alignment with the oriT_Tn916 |
oriT sequence
Download Length: 215 nt
>oriT_ICESpnTw19F14-1
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 259 | GenBank | ACO22579 |
| Name | Relaxase_ICESpnTw19F14-1 |
UniProt ID | _ |
| Length | 401 a.a. | PDB ID | |
| Note | transcriptional regulator, Cro/CI family | ||
Relaxase protein sequence
Download Length: 401 a.a. Molecular weight: 47398.10 Da Isoelectric Point: 6.5091
>ACO22579.1 transcriptional regulator, Cro/CI family [Streptococcus pneumoniae Taiwan19F-14]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 259 | GenBank | ACO23578 |
| Name | T4CP_ICESpnTw19F14-1 |
UniProt ID | _ |
| Length | 461 a.a. | PDB ID | _ |
| Note | ftsk/spoiiie family protein | ||
T4CP protein sequence
Download Length: 461 a.a. Molecular weight: 53370.27 Da Isoelectric Point: 9.0687
>ACO23578.1 ftsk/spoiiie family protein [Streptococcus pneumoniae Taiwan19F-14]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1785496..1796906
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SPT_1900 | 1780883..1781899 | - | 1017 | ACO22516 | regulator of lytrabc operon | - |
| SPT_1901 | 1781907..1782425 | - | 519 | ACO22979 | acetyltransferase, gnat family | - |
| SPT_1902 | 1782415..1782858 | - | 444 | ACO23444 | conserved hypothetical protein | - |
| SPT_1903 | 1782944..1783564 | - | 621 | ACO23895 | lipoprotein, putative | - |
| SPT_1904 | 1783870..1784733 | - | 864 | ACO22280 | transcriptional activator | - |
| SPT_1905 | 1784924..1785094 | + | 171 | ACO23716 | conserved hypothetical protein | - |
| SPT_1906 | 1785354..1785473 | + | 120 | ACO23093 | hypothetical protein | - |
| SPT_1907 | 1785496..1785810 | + | 315 | ACO22681 | conjugative transposon protein | orf23 |
| SPT_1908 | 1785829..1786212 | + | 384 | ACO24063 | conjugative transposon protein | orf23 |
| SPT_1909 | 1786241..1787626 | + | 1386 | ACO23578 | ftsk/spoiiie family protein | virb4 |
| SPT_1910 | 1787804..1789009 | + | 1206 | ACO22579 | transcriptional regulator, Cro/CI family | - |
| SPT_1911 | 1789052..1789273 | + | 222 | ACO23012 | conserved domain protein | orf19 |
| SPT_1912 | 1789390..1789887 | + | 498 | ACO22545 | conjugative transposon protein | - |
| SPT_1913 | 1789862..1790368 | + | 507 | ACO24205 | conjugative transposon membrane protein | orf17a |
| SPT_1914 | 1790352..1792799 | + | 2448 | ACO23752 | conjugative transposon protein | virb4 |
| SPT_1915 | 1792802..1794979 | + | 2178 | ACO22770 | conjugative transposon membrane protein | orf15 |
| SPT_1916 | 1794976..1795977 | + | 1002 | ACO22322 | NLP/P60 family protein | orf14 |
| SPT_1917 | 1795974..1796906 | + | 933 | ACO22536 | conjugative transposon protein | orf13 |
| SPT_1918 | 1797181..1797267 | + | 87 | ACO22508 | tetracycline resistance determinant leader peptide | - |
| SPT_1919 | 1797283..1799202 | + | 1920 | ACO23503 | tetracycline resistance protein | - |
| SPT_1922 | 1799808..1800143 | + | 336 | ACO23145 | conserved hypothetical protein | - |
| SPT_1923 | 1800213..1800524 | + | 312 | ACO23868 | conserved hypothetical protein | - |
| SPT_1924 | 1800511..1800810 | + | 300 | ACO22241 | conserved hypothetical protein | - |
Host bacterium
| ID | 50 | Element type | Transposon |
| Element name | ICESpnTw19F14-1 | GenBank | CP000921 |
| Element size | 2112148 bp | Coordinate of oriT [Strand] | 1787601..1787815 [+] |
| Host bacterium | Streptococcus pneumoniae Taiwan19F-14 | Coordinate of element | 1785161..1808701 |
Cargo genes
| Drug resistance gene | tet(M), msr(D), mef(A) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |