Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200041 |
| Name | oriT_ICESorUo5-1  |
| Organism | Streptococcus oralis Uo5 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | FR720602 (1826945..1827159 [+], 215 nt) |
| oriT length | 215 nt |
| IRs (inverted repeats) | 61..76, 80..95 (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) 123..134, 139..150 (GAAAATCCTTTG..CAAGGGATTTAC) |
| Location of nic site | 135..136 |
| Conserved sequence flanking the nic site |
TGG|T |
| Note | blastn alignment with the oriT_Tn916 |
oriT sequence
Download Length: 215 nt
>oriT_ICESorUo5-1
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 255 | GenBank | CBZ01516 |
| Name | Relaxase_ICESorUo5-1 |
UniProt ID | F2QFU7 |
| Length | 401 a.a. | PDB ID | |
| Note | Tn916, transcriptional regulator, putative | ||
Relaxase protein sequence
Download Length: 401 a.a. Molecular weight: 47398.10 Da Isoelectric Point: 6.5091
>CBZ01516.1 Tn916, transcriptional regulator, putative [Streptococcus oralis Uo5]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | F2QFU7 |
T4CP
| ID | 255 | GenBank | CBZ01515 |
| Name | T4CP_ICESorUo5-1 |
UniProt ID | F2QFU6 |
| Length | 389 a.a. | PDB ID | _ |
| Note | Tn916, FtsK/SpoIIIE family protein | ||
T4CP protein sequence
Download Length: 389 a.a. Molecular weight: 45150.43 Da Isoelectric Point: 8.5669
>CBZ01515.1 Tn916, FtsK/SpoIIIE family protein [Streptococcus oralis Uo5]
MLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGL
IQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMK
NVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLLSC
IETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLGRQ
AGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVIS
EFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
MLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGL
IQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMK
NVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLLSC
IETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLGRQ
AGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVIS
EFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | F2QFU6 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1824853..1836253
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SOR_1861 | 1820340..1822190 | - | 1851 | CBZ01508 | putative nucleoside-diphosphate sugar isomerase | - |
| SOR_1862 | 1822266..1823096 | - | 831 | CBZ01509 | glycosyl transferase family 2 | - |
| SOR_1863 | 1823318..1824484 | + | 1167 | CBZ01510 | putative cyanate permease | - |
| SOR_1864 | 1824711..1824830 | + | 120 | CBZ01511 | Tn916 hypothetical protein | - |
| SOR_1865 | 1824853..1825167 | + | 315 | CBZ01512 | Tn916 conserved hypothetical protein | orf23 |
| SOR_1866 | 1825183..1825569 | + | 387 | CBZ01513 | Tn916 conserved hypothetical protein | orf23 |
| SOR_1867 | 1825598..1825768 | + | 171 | CBZ01514 | Tn916, FtsK/SpoIIIE family protein | - |
| SOR_1868 | 1825801..1826970 | + | 1170 | CBZ01515 | Tn916, FtsK/SpoIIIE family protein | virb4 |
| SOR_1869 | 1827148..1828353 | + | 1206 | CBZ01516 | Tn916, transcriptional regulator, putative | - |
| SOR_1870 | 1828396..1828617 | + | 222 | CBZ01517 | Tn916 conserved hypothetical protein | orf19 |
| SOR_1871 | 1828734..1829231 | + | 498 | CBZ01518 | Tn916 conserved hypothetical protein | - |
| SOR_1872 | 1829320..1829712 | + | 393 | CBZ01519 | Tn916 conserved hypothetical protein | orf17a |
| SOR_1873 | 1829696..1832143 | + | 2448 | CBZ01520 | Tn916 conserved hypothetical protein | virb4 |
| SOR_1874 | 1832212..1834323 | + | 2112 | CBZ01521 | Tn916 conserved hypothetical protein | orf15 |
| SOR_1875 | 1834320..1835321 | + | 1002 | CBZ01522 | Tn916 protein; NLP/P60 family protein | orf14 |
| SOR_1876 | 1835336..1836253 | + | 918 | CBZ01523 | Tn916 conserved hypothetical protein | orf13 |
| SOR_1877 | 1836631..1838550 | + | 1920 | CBZ01524 | tetracycline resistance protein | - |
| SOR_1878 | 1838896..1838979 | - | 84 | CBZ01525 | Tn916 conserved hypothetical protein; transcriptional regulator, putative | - |
| SOR_1879 | 1839449..1839532 | + | 84 | CBZ01526 | 23S rRNA methylase leader peptide | - |
| SOR_1880 | 1839657..1840394 | + | 738 | CBZ01527 | erythromycin resistance protein; ribosomal RNA adenine N-6-methyltransferaseadenine dimethylase family protein | - |
Host bacterium
| ID | 46 | Element type | Transposon |
| Element name | ICESorUo5-1 | GenBank | FR720602 |
| Element size | 1958690 bp | Coordinate of oriT [Strand] | 1826945..1827159 [+] |
| Host bacterium | Streptococcus oralis Uo5 | Coordinate of element | 1824518..1847810 |
Cargo genes
| Drug resistance gene | tet(M), erm(B) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |