Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200040
• Name: oriT_ICECdiM68-1
• Organism: Clostridioides difficile M68
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_017175 (337912..338095 [+], 184 nt)
• oriT length: 184 nt
• IRs (inverted repeats): 1..11, 16..26 (GCTGGTGTGGC..GCCACGCCAGC) 92..103, 108..119 ( GAAAATCCTTTG..CAAGGGATTTAC)
• Location of nic site: 104..105
• Conserved sequence flanking the nic site: TGG|T
• Note: blastn alignment with the oriT_Tn916

oriT sequence

• Length: 184 nt

>oriT_ICECdiM68-1
GCTGGTGTGGCGTAAGCCACGCCAGCAGGTAAAACCCCTCGGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_ICECdiM68-1

• ID: 254
• GenBank: WP_000398284
• Name: Relaxase_ICECdiM68-1
• UniProt ID: _
• Length: 401 a.a.
• Note: _

Relaxase protein sequence

• Length: 401 a.a.
• Molecular weight: 47398.10 Da
• Isoelectric Point: 6.5091

>WP_000398284.1 MULTISPECIES: MobT family relaxase [Bacteria]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK

Protein domains

Predicted by InterproScan.

• Helix-turn-helix (HTH_3) (15-53)

• Replication initiation factor (Rep_trans) (169-373)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (73-161)

Protein structure

No available structure.

T4CP

T4CP_ICECdiM68-1

• ID: 254
• GenBank: WP_044593066
• Name: T4CP_ICECdiM68-1
• UniProt ID: _
• Length: 464 a.a.
• PDB ID: _
• Note: cell division protein FtsK

T4CP protein sequence

• Length: 464 a.a.
• Molecular weight: 53309.79 Da
• Isoelectric Point: 6.8996

>WP_044593066.1 MULTISPECIES: FtsK/SpoIIIE domain-containing protein [Clostridia]
MRMIWNKGHRIRASDKHLVYHFSIGTLLFVFVAVLLLLNSKQLMCTDWEHFSLLENGLTLSPYNFITILI
ATGVCALVAFLYYRFCYDSFKKLLHRQKLARMVLENKWYEADTVQDSIFFTDLQSRSREKIVWFPKIYYQ
MEKGLLHIRCEITLGKYQDQLLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGC
LRLMKNLVWEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVYHTKE
EMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEMLGTKESVSLLSQLKKIV
MLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISELGYGMLFGSDVKKQFFQKRIKGRGYCDVG
TSVISEFYTPLVPKGHDFLQTIGPLAQARQDVTATCEAKGDGTD

Protein domains

No domain identified.

Protein structure

No available structure.

Host bacterium

• ID: 45
• Element type: Transposon
• Element name: ICECdiM68-1
• GenBank: NC_017175
• Element size: 4308325 bp
• Coordinate of oriT [Strand]: 337912..338095 [+]
• Host bacterium: Clostridioides difficile M68
• Coordinate of element: 334783..353473

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -