Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200037 |
| Name | oriT_ICESpsHKU1003-1  |
| Organism | Staphylococcus pseudintermedius HKU10-03 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | CP002439 (938841..939055 [-], 215 nt) |
| oriT length | 215 nt |
| IRs (inverted repeats) | 61..76, 80..95 (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) 123..134, 139..150 (GAAAATCCTTTG..CAAGGGATTTAC) |
| Location of nic site | 135..136 |
| Conserved sequence flanking the nic site |
TGG|T |
| Note | blastn alignment with the oriT_Tn916 |
oriT sequence
Download Length: 215 nt
>oriT_ICESpsHKU1003-1
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 251 | GenBank | ADV06648 |
| Name | Relaxase_ICESpsHKU1003-1 |
UniProt ID | E8SFV0 |
| Length | 401 a.a. | PDB ID | |
| Note | Tn916-associated transcriptional regulator | ||
Relaxase protein sequence
Download Length: 401 a.a. Molecular weight: 47398.10 Da Isoelectric Point: 6.5091
>ADV06648.1 Tn916-associated transcriptional regulator [Staphylococcus pseudintermedius HKU10-03]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 251 | GenBank | ADV06649 |
| Name | T4CP_ICESpsHKU1003-1 |
UniProt ID | E8SFV1 |
| Length | 461 a.a. | PDB ID | _ |
| Note | Cell division FtsK/SpoIIIE protein | ||
T4CP protein sequence
Download Length: 461 a.a. Molecular weight: 53370.27 Da Isoelectric Point: 9.0687
>ADV06649.1 Cell division FtsK/SpoIIIE protein [Staphylococcus pseudintermedius HKU10-03]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2201927..2213178
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SPSINT_2109 | 2197115..2197318 | - | 204 | ADV06637 | Tn916 ORF1- like transposon excisionase | - |
| SPSINT_2110 | 2198006..2198428 | - | 423 | ADV06638 | Tn916-associated hypothetical protein | - |
| SPSINT_2111 | 2198932..2199285 | + | 354 | ADV06639 | Tn916-associated transcriptional regulator | - |
| SPSINT_2112 | 2199631..2201550 | - | 1920 | ADV06640 | Tetracycline resistance protein TetM | - |
| SPSINT_2113 | 2201927..2202859 | - | 933 | ADV06641 | conserved hypothetical protein | orf13 |
| SPSINT_2114 | 2202856..2203857 | - | 1002 | ADV06642 | lipoprotein, NLP/P60 family | orf14 |
| SPSINT_2115 | 2203854..2206031 | - | 2178 | ADV06643 | hypothetical membrane protein | orf15 |
| SPSINT_2116 | 2206034..2208481 | - | 2448 | ADV06644 | hypothetical protein | virb4 |
| SPSINT_2117 | 2208465..2208857 | - | 393 | ADV06645 | Tn916-associated hypothetical protein | orf17a |
| SPSINT_2118 | 2208946..2209443 | - | 498 | ADV06646 | Antirestriction protein | - |
| SPSINT_2119 | 2209560..2209781 | - | 222 | ADV06647 | Conjugation related protein | orf19 |
| SPSINT_2120 | 2209824..2211029 | - | 1206 | ADV06648 | Tn916-associated transcriptional regulator | - |
| SPSINT_2121 | 2211207..2212592 | - | 1386 | ADV06649 | Cell division FtsK/SpoIIIE protein | virb4 |
| SPSINT_2122 | 2212621..2213007 | - | 387 | ADV06650 | hypothetical protein | orf23 |
| SPSINT_2123 | 2213023..2213178 | - | 156 | ADV06651 | hypothetical protein | orf23 |
| SPSINT_2124 | 2213663..2213908 | + | 246 | ADV06652 | hypothetical protein | - |
| SPSINT_2125 | 2214798..2216657 | - | 1860 | ADV06653 | hypothetical ABC transporter | - |
Host bacterium
| ID | 42 | Element type | Transposon |
| Element name | ICESpsHKU1003-1 | GenBank | CP002439 |
| Element size | 2617381 bp | Coordinate of oriT [Strand] | 938841..939055 [-] |
| Host bacterium | Staphylococcus pseudintermedius HKU10-03 | Coordinate of element | 2195642..2213672 |
Cargo genes
| Drug resistance gene | tet(M) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |