Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200032 |
| Name | oriT_ICESga43143-1  |
| Organism | Streptococcus gallolyticus subsp. gallolyticus ATCC 43143 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | AP012053 (1658753..1658967 [-], 215 nt) |
| oriT length | 215 nt |
| IRs (inverted repeats) | 61..76, 80..95 (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) 123..134, 139..150 (GAAAATCCTTTG..CAAGGGATTTAC) |
| Location of nic site | 135..136 |
| Conserved sequence flanking the nic site |
TGG|T |
| Note | blastn alignment with the oriT_Tn916 |
oriT sequence
Download Length: 215 nt
>oriT_ICESga43143-1
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 246 | GenBank | BAK28456 |
| Name | Relaxase_ICESga43143-1 |
UniProt ID | F5WVZ9 |
| Length | 401 a.a. | PDB ID | |
| Note | Tn916 ORF20 phage replication initiation factor PEP | ||
Relaxase protein sequence
Download Length: 401 a.a. Molecular weight: 47398.10 Da Isoelectric Point: 6.5091
>BAK28456.1 Tn916 ORF20 phage replication initiation factor PEP [Streptococcus gallolyticus subsp. gallolyticus ATCC 43143]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | F5WVZ9 |
T4CP
| ID | 246 | GenBank | BAK28457 |
| Name | T4CP_ICESga43143-1 |
UniProt ID | F5WW00 |
| Length | 461 a.a. | PDB ID | _ |
| Note | Tn916 ORF21 FtsK/SpoIIIE family protein | ||
T4CP protein sequence
Download Length: 461 a.a. Molecular weight: 53370.27 Da Isoelectric Point: 9.0687
>BAK28457.1 Tn916 ORF21 FtsK/SpoIIIE family protein [Streptococcus gallolyticus subsp. gallolyticus ATCC 43143]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | F5WW00 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1649662..1661072
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SGGB_1587 | 1644849..1645052 | - | 204 | BAK28445 | Tn916 ORF1 excisionase | - |
| SGGB_1588 | 1645740..1646162 | - | 423 | BAK28446 | Tn916 ORF7 DNA-binding protein protein | - |
| SGGB_1589 | 1646667..1647020 | + | 354 | BAK28447 | Tn916 ORF9 transcriptional regulator, putative | - |
| SGGB_1590 | 1647366..1649285 | - | 1920 | BAK28448 | Tn916 ORF11 tetracycline resistance protein | - |
| SGGB_1591 | 1649662..1650594 | - | 933 | BAK28449 | Tn916 ORF13 protein | orf13 |
| SGGB_1592 | 1650591..1651592 | - | 1002 | BAK28450 | Tn916 ORF14 extracellular hydrolase/peptidase | orf14 |
| SGGB_1593 | 1651589..1653766 | - | 2178 | BAK28451 | Tn916 ORF15 signal peptide containing protein | orf15 |
| SGGB_1594 | 1653769..1656216 | - | 2448 | BAK28452 | Tn916 ORF16 ATP/GTP-binding protein | virb4 |
| SGGB_1595 | 1656200..1656706 | - | 507 | BAK28453 | Tn916 ORF17 signal peptide containing protein | orf17a |
| SGGB_1596 | 1656681..1657178 | - | 498 | BAK28454 | Tn916 ORF18 antirestriction (ArdA) protein | - |
| SGGB_1597 | 1657295..1657516 | - | 222 | BAK28455 | Tn916 ORF19 protein | orf19 |
| SGGB_1598 | 1657559..1658764 | - | 1206 | BAK28456 | Tn916 ORF20 phage replication initiation factor PEP | - |
| SGGB_1599 | 1658942..1660327 | - | 1386 | BAK28457 | Tn916 ORF21 FtsK/SpoIIIE family protein | virb4 |
| SGGB_1600 | 1660356..1660742 | - | 387 | BAK28458 | Tn916 ORF22 protein | orf23 |
| SGGB_1601 | 1660758..1661072 | - | 315 | BAK28459 | Tn916 ORF23 protein | orf23 |
| SGGB_1602 | 1661408..1663177 | - | 1770 | BAK28460 | cell wall surface protein | - |
| SGGB_1603 | 1663234..1665144 | - | 1911 | BAK28461 | PTS system, beta-glucosides-specific IIABC component | - |
Host bacterium
| ID | 37 | Element type | Transposon |
| Element name | ICESga43143-1 | GenBank | AP012053 |
| Element size | 2362241 bp | Coordinate of oriT [Strand] | 1658753..1658967 [-] |
| Host bacterium | Streptococcus gallolyticus subsp. gallolyticus ATCC 43143 | Coordinate of element | 1643376..1661407 |
Cargo genes
| Drug resistance gene | tet(M) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |