Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200027 |
| Name | oriT_ICESpn11930-2  |
| Organism | Streptococcus pneumoniae Tn916-type integrative and |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | FR671416 (2106..2320 [+], 215 nt) |
| oriT length | 215 nt |
| IRs (inverted repeats) | 61..76, 80..95 (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) 120..132, 140..153 ( TTAGAAAATCCTT..AAGGGATTTACAAA) |
| Location of nic site | 135..136 |
| Conserved sequence flanking the nic site |
TGG|T |
| Note | blastn alignment with the oriT_Tn916 |
oriT sequence
Download Length: 215 nt
>oriT_ICESpn11930-2
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTATAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTATAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 241 | GenBank | CBW39386 |
| Name | Orf20_ICESpn11930-2 |
UniProt ID | B7UTY4 |
| Length | 472 a.a. | PDB ID | |
| Note | _ | ||
Relaxase protein sequence
Download Length: 472 a.a. Molecular weight: 55639.89 Da Isoelectric Point: 8.3884
>CBW39386.1 putative conjugative transposon replication initiation factor [Streptococcus pneumoniae]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKKGYLSTIPVDRYPKKDIMGD
KTVRVRADLHHIIKIETAKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKKGYLSTIPVDRYPKKDIMGD
KTVRVRADLHHIIKIETAKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B7UTY4 |
T4CP
| ID | 241 | GenBank | CBW39385 |
| Name | Orf21_ICESpn11930-2 |
UniProt ID | B7UTY5 |
| Length | 461 a.a. | PDB ID | _ |
| Note | conjugative transposon FtsK/SpoIIIE-family protein | ||
T4CP protein sequence
Download Length: 461 a.a. Molecular weight: 53370.27 Da Isoelectric Point: 9.0687
>CBW39385.1 conjugative transposon FtsK/SpoIIIE-family protein [Streptococcus pneumoniae]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B7UTY5 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1..14262
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_0 | 1..315 | + | 315 | CBW39383 | conjugative transposon protein | orf23 |
| Locus_1 | 331..717 | + | 387 | CBW39384 | conjugative transposon protein | orf23 |
| Locus_2 | 746..2131 | + | 1386 | CBW39385 | conjugative transposon FtsK/SpoIIIE-family protein | virb4 |
| Locus_3 | 2309..3727 | + | 1419 | CBW39386 | putative conjugative transposon replication initiation factor | - |
| Locus_4 | 3984..4721 | + | 738 | CBW39387 | rRNA methylase | - |
| Locus_5 | 4978..6225 | - | 1248 | CBW39388 | Transposase | - |
| Locus_6 | 6459..6626 | + | 168 | CBW39389 | conjugative transposon protein | orf19 |
| Locus_7 | 6743..7240 | + | 498 | CBW39390 | conjugative transposon protein | - |
| Locus_8 | 7491..7721 | + | 231 | CBW39391 | putative conjugative transposon membrane protein | orf17a |
| Locus_9 | 7705..10152 | + | 2448 | CBW39392 | conjugative transposon ATP/GTP-binding protein | virb4 |
| Locus_10 | 10221..12332 | + | 2112 | CBW39393 | conjugative transposon membrane protein | orf15 |
| Locus_11 | 12329..13330 | + | 1002 | CBW39394 | putative cell wall hydrolase | orf14 |
| Locus_12 | 13327..14262 | + | 936 | CBW39395 | putative conjugative transposon exported protein | orf13 |
| Locus_13 | 14624..16558 | + | 1935 | CBW39396 | conjugative transposon tetracycline resistance protein | - |
| Locus_15 | 17665..18402 | + | 738 | CBW39398 | rRNA methylase | - |
Host bacterium
| ID | 32 | Element type | Transposon |
| Element name | ICESpn11930-2 | GenBank | FR671416 |
| Element size | 25645 bp | Coordinate of oriT [Strand] | 2106..2320 [+] |
| Host bacterium | Streptococcus pneumoniae Tn916-type integrative and |
Cargo genes
| Drug resistance gene | erm(B), tet(M) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |