Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200026
• Name: oriT_ICESpn23771
• Organism: Streptococcus pneumoniae Tn916-type integrative and
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): FR671415 (2106..2320 [+], 215 nt)
• oriT length: 215 nt
• IRs (inverted repeats): 61..76, 80..95 (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) 123..134, 139..150 (GAAAATCCTTTG..CAAGGGATTTAC)
• Location of nic site: 135..136
• Conserved sequence flanking the nic site: TGG|T
• Note: blastn alignment with the oriT_Tn916

oriT sequence

• Length: 215 nt

>oriT_ICESpn23771
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGGATTT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Orf20_ICESpn23771

• ID: 240
• GenBank: CBW39362
• Name: Orf20_ICESpn23771
• UniProt ID: B7UTY4
• Length: 472 a.a.
• Note: _

Relaxase protein sequence

• Length: 472 a.a.
• Molecular weight: 55639.89 Da
• Isoelectric Point: 8.3884

>CBW39362.1 putative conjugative transposon replication initiation factor [Streptococcus pneumoniae]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKKGYLSTIPVDRYPKKDIMGD
KTVRVRADLHHIIKIETAKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN

Protein domains

Predicted by InterproScan.

• Replication initiation factor (Rep_trans) (169-373)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (73-161)

• Helix-turn-helix (HTH_3) (15-53)

• Omega Transcriptional Repressor (Omega_Repress) (414-453)

Protein structure

• AlphaFold DB: B7UTY4

T4CP

Orf21_ICESpn23771

• ID: 240
• GenBank: CBW39361
• Name: Orf21_ICESpn23771
• UniProt ID: B7UTY5
• Length: 461 a.a.
• PDB ID: _
• Note: conjugative transposon FtsK/SpoIIIE-family protein

T4CP protein sequence

• Length: 461 a.a.
• Molecular weight: 53370.27 Da
• Isoelectric Point: 9.0687

>CBW39361.1 conjugative transposon FtsK/SpoIIIE-family protein [Streptococcus pneumoniae]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (217-301)

Protein structure

• AlphaFold DB: B7UTY5

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1..14259

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Locus_0	1..315	+	315	CBW39359	conjugative transposon protein	orf23
Locus_1	334..717	+	384	CBW39360	conjugative transposon protein	orf23
Locus_2	746..2131	+	1386	CBW39361	conjugative transposon FtsK/SpoIIIE-family protein	virb4
Locus_3	2309..3727	+	1419	CBW39362	putative conjugative transposon replication initiation factor	-
Locus_4	3984..4721	+	738	CBW39363	rRNA methylase	-
Locus_5	4978..6225	-	1248	CBW39364	Transposase	-
Locus_6	6405..6626	+	222	CBW39365	conjugative transposon protein	orf19
Locus_7	6743..7240	+	498	CBW39366	conjugative transposon protein	-
Locus_8	7491..7721	+	231	CBW39367	putative conjugative transposon membrane protein	orf17a
Locus_9	7705..10152	+	2448	CBW39368	conjugative transposon ATP/GTP-binding protein	virb4
Locus_10	10155..12332	+	2178	CBW39369	conjugative transposon membrane protein	orf15
Locus_11	12329..13330	+	1002	CBW39370	putative cell wall hydrolase	orf14
Locus_12	13345..14259	+	915	CBW39371	putative conjugative transposon exported protein	orf13
Locus_13	14636..16555	+	1920	CBW39372	conjugative transposon tetracycline resistance protein	-
Locus_14	16767..17164	+	398	CBW39373	UmuC/MucB-like protein	-
Locus_15	17161..17496	+	336	CBW39374	ImsC-like protein	-
Locus_16	17509..17877	+	369	CBW39375	YolD domain protein	-

Host bacterium

• ID: 31
• Element type: Transposon
• Element name: ICESpn23771
• GenBank: FR671415
• Element size: 25880 bp
• Coordinate of oriT [Strand]: 2106..2320 [+]
• Host bacterium: Streptococcus pneumoniae Tn916-type integrative and

Cargo genes

• Drug resistance gene: erm(B), tet(M), msr(D), mef(A)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -