Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200011 |
| Name | oriT_NBU2  |
| Organism | Bacteroides sp. mobilization protein |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | L42370 (_) |
| oriT length | 219 nt |
| IRs (inverted repeats) | 166..173, 178..185 (AGTTATAG..CTATAACT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | similar to NBU1 oriT region |
oriT sequence
Download Length: 219 nt
TCCGCATGGAATACGGCAGAGAGCGGTACATCCGGGACGCTTCGCAGATTTACAGAGGATGCAAGGACTTGAACGAATACTTACAGAAACAGATTGAAAGAAAAAGGCAGCTCCAGTCCGCCAAAGGGGTGCGCAGCCAATCACCGGAAAAGAAGAACGGCTTCCAGTTATAGCCCACTATAACTACACGCTTCGCTTTCGTAGTTGTGGGCTCTCCCGGGG
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Wang J et al. (2000) Characterization of a Bacteroides mobilizable transposon, NBU2, which carries a functional lincomycin resistance gene. J Bacteriol. 182(12):3559-71. [PMID:10852890]
[2] Li LY et al. (1995) The mobilization regions of two integrated Bacteroides elements, NBU1 and NBU2, have only a single mobilization protein and may be on a cassette. J Bacteriol. 177(14):3940-5. [PMID:7608064]
Relaxase
| ID | 225 | GenBank | AAA87350 |
| Name | Mob_NBU2  |
UniProt ID | Q45639 |
| Length | 467 a.a. | PDB ID | |
| Note | _ | ||
Relaxase protein sequence
Download Length: 467 a.a. Molecular weight: 54873.78 Da Isoelectric Point: 9.7980
MATKSSIHIKPCNIASSEAHNRRTAEYMRNIGESRIYVVPELSTDNEQWINPDFGTPELRTHYDNIKQMV
KEKTGRAMQEKERERKGKNGKIIKVAGCSPIREGVLLIRPDTTLADVRKFGEECQRRWGITPLQIFLHKD
EGHWLNGQPEAEDKESFQVGNRWFKPNYHAHVVFDWMNHETGKSRKLNDEDMATMQTLASDILLMERGQA
KAVTGKEHLERNDFIIEKQKAELQRIEETKRHKEQQVSLAEQELKQVKAEIRTDKLKKTATNAATAIASG
VGSLFGSGKLKELEHHIEQLHQEITKRDKATDELKIQIQQIQEQHSKQIRNLQRIHNQELEAKDKEISRL
STLLEKAHKWFPMFKEMLRMEKLCAVIGFTKDMIENLLTKKESIQCSGKIYSEEHRWKFDIKNDIFRLEK
HPMDSWKLMLTINRQPIVQWFKEQWEKLQQNLRNSVQREQKSRGFRI
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q45639 |
Reference
[1] Li LY et al. (1995) The mobilization regions of two integrated Bacteroides elements, NBU1 and NBU2, have only a single mobilization protein and may be on a cassette. J Bacteriol. 177(14):3940-5. [PMID:7608064]
Host bacterium
| ID | 16 | Element type | IME (Integrative mobilizable element) |
| Element name | NBU2 | GenBank | L42370 |
| Element size | 2566 bp | Coordinate of oriT [Strand] | |
| Host bacterium | Bacteroides sp. mobilization protein |
Cargo genes
| Drug resistance gene | carries a functional lincomycin resistance gene |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |