Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200010 |
| Name | oriT_NBU1  |
| Organism | Bacteroides uniformis |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_006373 (8213..8426 [+], 214 nt) |
| oriT length | 214 nt |
| IRs (inverted repeats) | 143..149, 162..168 (ACCGAAA..TTTCGGT) 182..187, 198..203 (AACTAC..GTAGTT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | _ |
oriT sequence
Download Length: 214 nt
TCCGCAAGGAATACAGCAGAGACCGATACATCCGTGACGCTTCACAGATTTACAGCGGATGCAAGGACTTGAACGAATACTTACAGAAACAGGTTGAAAGAAAAAGGCAAGTCCAATCCGTCAAAGGGATGAGCAGCCAGTCACCGAAAAAGAAAAACGGCTTTCGGTTATAGCCCACTATAACTACCTCCGCTTTCGTAGTTGTGGGCTCTCCGAGGGGA
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Vedantam G et al. (2006) Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation. Mol Microbiol. 59(1):288-300. [PMID:16359335]
[2] Wang J et al. (2000) Characterization of a Bacteroides mobilizable transposon, NBU2, which carries a functional lincomycin resistance gene. J Bacteriol. 182(12):3559-71. [PMID:10852890]
[3] Li LY et al. (1995) The mobilization regions of two integrated Bacteroides elements, NBU1 and NBU2, have only a single mobilization protein and may be on a cassette. J Bacteriol. 177(14):3940-5. [PMID:7608064]
Relaxase
| ID | 224 | GenBank | YP_133705 |
| Name | MobN1_NBU1  |
UniProt ID | _ |
| Length | 467 a.a. | PDB ID | |
| Note | NBU1 mobilization protein | ||
Relaxase protein sequence
Download Length: 467 a.a. Molecular weight: 54421.32 Da Isoelectric Point: 10.0229
MATKSSIHIKPCNIASSEVHNRRTAEYMRNIGESRIYVVPELSTNNEQWINPDFGTPELRTHYDNIKQMV
KEKTGRAMQEKERERKGKNGKIIKVAGCSPIREGVLLIRPDTTLADVRKFGEECQRRWGITPLQIFLHKD
EGHWLNGQPEAEDRESFKVGNRWFKPNYHAHIVFDWMNHETGKSRKLNDEDMAAMQTLASNILLMERGQA
KAVTGKEHLERNDFIIKKQKAELQRIEETKRHKEQQVSLAEQELKQVKAEIRTDKLKSAATDVATAITSG
VGSLFGSGKLKDLEQSNENLRHEIAKRDKGIDELKAKMQQMQEQHSKQIRNLQGIHNQELEAKDKEISRL
NAILEKAFNWFPLLKEMLRMEKLCYAIGFTKDMINSLLTKKEAIRCNGRIYSEEHKRKFDIKNDIFKVEK
NPTDDSKLILTINRQPIGEWFKEQWEKLRQGLRQLAEEPRKSRGFRM
Protein domains
No domain identified.
Protein structure
No available structure.
Host bacterium
| ID | 15 | Element type | IME (Integrative mobilizable element) |
| Element name | NBU1 | GenBank | NC_006373 |
| Element size | 10276 bp | Coordinate of oriT [Strand] | 8213..8426 [+] |
| Host bacterium | Bacteroides uniformis |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |