Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200007 |
| Name | oriT_Tn5520  |
| Organism | Bacteroides fragilis |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | AF038866 (2321..2391 [+], 71 nt) |
| oriT length | 71 nt |
| IRs (inverted repeats) | 14..30, 55..71 (CTTATTAGGGAATTTTC..GAAAATTCCCCAATAAG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | 71 bp minimal oriT |
oriT sequence
Download Length: 71 nt
>oriT_Tn5520
CTTATTGGGGAATTTTCAGCGATACGGAGTATTGCGGCTCGGAAAATTCCCTAATAAGCTACGGTATTTTC
CTTATTGGGGAATTTTCAGCGATACGGAGTATTGCGGCTCGGAAAATTCCCTAATAAGCTACGGTATTTTC
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Vedantam G et al. (2006) Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation. Mol Microbiol. 59(1):288-300. [PMID:16359335]
Relaxase
| ID | 221 | GenBank | AAD24590 |
| Name | BmpH_Tn5520  |
UniProt ID | Q9X2P3 |
| Length | 455 a.a. | PDB ID | |
| Note | relaxase | ||
Relaxase protein sequence
Download Length: 455 a.a. Molecular weight: 52650.07 Da Isoelectric Point: 10.0660
>AAD24590.1 mobilization protein BmpH [Bacteroides fragilis]
MGFVVLHMEKAHGSDSGTTAHIERFIIPKNADPTHTYLNRRLIDYPDGVKDRSAAIQQRLEEAGLTRKIG
SNQVRAIRINVSGTHEDMKRIEEEGRLDEWCADNLKYFADTFGKENIVAAHLHRDEETPHIHVTLVPIVK
GERKRRKREEQTKKRYRKKPTNTVRLCADDIMTRLKLKSYQDTYAEAMAKYGLQRGIDGSKARHKSTQQY
YRDIQKLADNLKAEVVDLQQQKETAREELRRAKKEIQTEKLKGVATVAAANIAESVGSLFGSNKVKTLER
ENTALHREVADHEETIEALQDKIQTMQADHSRQMAEVERKHRREIADKETKHKEEISFLKTVIAKAAAWF
PYFREMLRIENLCRLIGFDERQTATLVKGKPLEYAGELYSEEHGRKFTTEKAGFQVVKDPTDGTRLVLAI
DRKPIAEWFKEQFEKVRQNIRRPIQLQKKGRGMKL
MGFVVLHMEKAHGSDSGTTAHIERFIIPKNADPTHTYLNRRLIDYPDGVKDRSAAIQQRLEEAGLTRKIG
SNQVRAIRINVSGTHEDMKRIEEEGRLDEWCADNLKYFADTFGKENIVAAHLHRDEETPHIHVTLVPIVK
GERKRRKREEQTKKRYRKKPTNTVRLCADDIMTRLKLKSYQDTYAEAMAKYGLQRGIDGSKARHKSTQQY
YRDIQKLADNLKAEVVDLQQQKETAREELRRAKKEIQTEKLKGVATVAAANIAESVGSLFGSNKVKTLER
ENTALHREVADHEETIEALQDKIQTMQADHSRQMAEVERKHRREIADKETKHKEEISFLKTVIAKAAAWF
PYFREMLRIENLCRLIGFDERQTATLVKGKPLEYAGELYSEEHGRKFTTEKAGFQVVKDPTDGTRLVLAI
DRKPIAEWFKEQFEKVRQNIRRPIQLQKKGRGMKL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q9X2P3 |
Reference
[1] Vedantam G et al. (2006) Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation. Mol Microbiol. 59(1):288-300. [PMID:16359335]
Host bacterium
| ID | 12 | Element type | IME (Integrative mobilizable element) |
| Element name | Tn5520 (chromosomal Tn) | GenBank | AF038866 |
| Element size | 4692 bp | Coordinate of oriT [Strand] | 2321..2391 [+] |
| Host bacterium | Bacteroides fragilis |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |