Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   122578
Name   oriT_pCV60 in_silico
Organism   Lactococcus lactis strain VHProbi V60
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP110852 (17285..17421 [+], 137 nt)
oriT length   137 nt
IRs (inverted repeats)      21..28, 42..49  (AAAAGGGG..CCCCTTTT)
 25..30, 39..44  (GGGGAA..TTCCCC)
Location of nic site      104..105
Conserved sequence flanking the
  nic site    		  GCTTGCAGTA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 137 nt

>oriT_pCV60
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGCTTTATATGGTCTATTTTGTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14241 GenBank   WP_039115941
Name   Relaxase_OHI04_RS12745_pCV60 insolico UniProt ID   _
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47758.36 Da        Isoelectric Point: 8.1264

>WP_039115941.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactococcus]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFRAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESTINRFIDTIKQFAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   23004 GenBank   NZ_CP110852
Plasmid name   pCV60 Incompatibility group   -
Plasmid size   18631 bp Coordinate of oriT [Strand]   17285..17421 [+]
Host baterium   Lactococcus lactis strain VHProbi V60

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -