Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 122573
• Name: oriT1_pARO1.4
• Organism: Enterococcus faecalis ARO1/DG
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP022487 (77..111 [+], 35 nt)
• oriT length: 35 nt
• IRs (inverted repeats): 1..6, 10..15 (CTTTAC..GTAAAG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 35 nt

>oriT1_pARO1.4
CTTTACGAAGTAAAGTATAGTGCGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_CG806_RS00935_pARO1.4

• ID: 14236
• GenBank: WP_010717414
• Name: mobV_CG806_RS00935_pARO1.4
• UniProt ID: Q9ZEH9
• Length: 559 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 559 a.a.
• Molecular weight: 65102.51 Da
• Isoelectric Point: 7.9635

>WP_010717414.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYDLVNPGSINYREKIKQIIESQRISKR
AVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDVVAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYE
GRLSSKQVFSRQNLLEIQEELPTYLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQIL
AEKERELHSYEQAIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANVKEKKFSLNGL
KYVLSASDFSAIKSITKDFETLRTQNKHLKIENNNLAQKNKELTQVNHEQELQITELAYELTLKKEQLTA
FEEKQLNYDQSEQIKQELDTTTQTVSVLETENKRLKDKITDLTYDFSELNEKYQEIKLKFDGLTTYLHER
LGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTYLIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKA
PFYNRETGCYEFVGEYMANGRSRTNTLQLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (2-191)

Protein structure

• AlphaFold DB: Q9ZEH9

Host bacterium

• ID: 22999
• GenBank: NZ_CP022487
• Plasmid name: pARO1.4
• Incompatibility group: -
• Plasmid size: 5121 bp
• Coordinate of oriT [Strand]: 77..111 [+]; 4607..4656 [-]
• Host baterium: Enterococcus faecalis ARO1/DG

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -