Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   122572
Name   oriT1_LS170308|unnamed in_silico
Organism   Enterococcus faecium strain LS170308
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP025078 (36780..36817 [+], 38 nt)
oriT length   38 nt
IRs (inverted repeats)      1..6, 10..15  (CTTTAC..GTAAAG)
Location of nic site      24..25
Conserved sequence flanking the
  nic site  
 
 GTGTGTTATA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 38 nt

>oriT1_LS170308|unnamed
CTTTACGAAGTAAAGTATAGTGTGTTATACTTTACATG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   14235 GenBank   WP_002328823
Name   mobV_CWE29_RS13670_LS170308|unnamed insolico UniProt ID   O32791
Length   403 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 403 a.a.        Molecular weight: 46815.76 Da        Isoelectric Point: 9.2672

>WP_002328823.1 MULTISPECIES: MobV family relaxase [Lactobacillales]
MSFAVARMQKVKSGNLVGVGNHNQRNTDNHSNKDIDVERSHLNYDLVNRTENYKRDIEQFINDNKSSSRA
VRKDAVLINEWIITSDNPFFKDKDDKEIKDFFDTAKSYFADKFGDNNIRYAQVHLDETTPHMHLGIVPFN
DEHKLSAKTVFNRQALQAVQDELPKYLNERGFELERGEKGSERKNLTVPEYKKAKDELKEITTTLEQRKS
EVLALSNDKAPTIKKESLDLKDETKTVKVPSGETIGIGKLRHEFMKNEERKTGNVIIPESKLNAVIKSYE
ELYKANEKLKNYAETDLPKEITRVKEKYRELAKDYNRLVRKSNQNIETLEELEKENKSLKNEIKGIYKGF
NQFMENTLGATVGQAKKLMNNLMSEVKEFVKGGEFEKIHRNETKTRDRDELSR

  Protein domains


Predicted by InterproScan.

(1-193)


  Protein structure


Source ID Structure
AlphaFold DB O32791


Host bacterium


ID   22998 GenBank   NZ_CP025078
Plasmid name   LS170308|unnamed Incompatibility group   -
Plasmid size   56373 bp Coordinate of oriT [Strand]   36780..36817 [+]; 38208..38233 [+]
Host baterium   Enterococcus faecium strain LS170308

Cargo genes


Drug resistance gene   lsa(E), lnu(B), ant(6)-Ia, erm(B), tet(M), tet(L), cat
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -