Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   122568
Name   oriT1_E8377|3 in_silico
Organism   Enterococcus faecium isolate E8377
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LR135403 (27904..27933 [-], 30 nt)
oriT length   30 nt
IRs (inverted repeats)      1..8, 13..20  (GGGAGTGT..ACACTCCC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 30 nt

>oriT1_E8377|3
GGGAGTGTAGCCACACTCCCTATGCTTGCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14231 GenBank   WP_002317279
Name   Relaxase_EQB54_RS16630_E8377|3 insolico UniProt ID   N2BU79
Length   442 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 442 a.a.        Molecular weight: 50173.57 Da        Isoelectric Point: 9.6754

>WP_002317279.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Terrabacteria group]
MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADIEFEMLLAQAYQKGNNLGHHLIQSFAP
GEATPEQAHEIGKQLAEEVLQGKYPYVLTTHIDKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDR
LCKENGLSVVKPGQSKGKSYAEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFI
SFRAPGQERFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWAKLHN
LKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLIKNISTYKQLWPVAMEY
RNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALKAEYKRLAEEKERLYEKYGEAKKQMQEYG
IIKQNVDGILRMTPGKEWTQEL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(10-240)


  Protein structure


Source ID Structure
AlphaFold DB N2BU79


Auxiliary protein

ID   7868 GenBank   WP_002317278
Name   WP_002317278_E8377|3 insolico UniProt ID   A0A0E2H681
Length   109 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 109 a.a.        Molecular weight: 12486.63 Da        Isoelectric Point: 10.5610

>WP_002317278.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Terrabacteria group]
MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLIDGYIVNMDTSDIRAQTAEIQKIGVNVN
QIAKRLNGMGPVYAQDIEDIKGALAQIWQLQRYILSSQR

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(60-99)


  Protein structure


Source ID Structure
AlphaFold DB A0A0E2H681


T4CP

ID   16491 GenBank   WP_002317293
Name   t4cp2_EQB54_RS16555_E8377|3 insolico UniProt ID   _
Length   602 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 602 a.a.        Molecular weight: 68060.13 Da        Isoelectric Point: 6.2196

>WP_002317293.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Terrabacteria group]
MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHFADGFAAAFANPLPSLHLFDLCIGIAG
AVIVRLVVYCKSKNVKKYRKGMEYGSARWGTAKDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNK
NVLVIGGSGSGKTRFFVKPNLMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSM
KYNPFVYIHSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTLLEMI
NASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISCGARLAPFDIREVRELM
EDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINLLCDRADDVYGGRLPVHVRLILDEFANIG
QIPNFDKLIATIRSREISASIILQSQSQLKAIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETID
SFNTSENRGSQTSHGLNYQKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADK
KNTFDVDRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(140-543)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 15430..24319

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB54_RS16525 11749..12564 + 816 WP_002326780 helix-turn-helix domain-containing protein -
EQB54_RS16530 12690..12947 + 258 WP_002326781 hypothetical protein -
EQB54_RS16535 13199..13675 - 477 Protein_16 recombinase family protein -
EQB54_RS16540 13922..15121 + 1200 WP_002317296 DUF6017 domain-containing protein -
EQB54_RS16545 15143..15355 + 213 WP_002317295 DUF5348 domain-containing protein -
EQB54_RS16550 15430..15906 + 477 WP_002317294 PcfB family protein cd411
EQB54_RS16555 15903..17711 + 1809 WP_002317293 VirD4-like conjugal transfer protein, CD1115 family -
EQB54_RS16560 17788..18003 + 216 WP_002317292 Maff2 family mobile element protein prgF
EQB54_RS16565 18095..18958 + 864 WP_002317291 VirB6/TrbL-like conjugal transfer protein, CD1112 family prgHa
EQB54_RS16570 18989..19531 + 543 WP_002317290 MT-A70 family methyltransferase -
EQB54_RS16575 19545..19967 + 423 WP_002370797 PrgI family protein prgIa
EQB54_RS16580 19897..22296 + 2400 WP_002317289 VirB4-like conjugal transfer ATPase, CD1110 family virb4
EQB54_RS16585 22328..24319 + 1992 WP_002382864 C40 family peptidase cd419a
EQB54_RS16590 24342..24593 + 252 WP_002317286 DUF4315 family protein -
EQB54_RS16595 24583..25812 + 1230 WP_002317285 CD1107 family mobile element protein -
EQB54_RS16600 25809..27890 + 2082 WP_002317284 DNA topoisomerase 3 -

Host bacterium

ID   22994 GenBank   NZ_LR135403
Plasmid name   E8377|3 Incompatibility group   -
Plasmid size   63798 bp Coordinate of oriT [Strand]   27904..27933 [-]; 35908..35935 [+]
Host baterium   Enterococcus faecium isolate E8377

Cargo genes

Drug resistance gene   VanHBX, erm(B), aph(3')-III, ant(6)-Ia
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -