Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   122566
Name   oriT1_E8290|3 in_silico
Organism   Enterococcus faecium isolate E8290
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LR135396 (9217..9246 [-], 30 nt)
oriT length   30 nt
IRs (inverted repeats)      1..8, 13..20  (GGGAGTGT..ACACTCCC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 30 nt

>oriT1_E8290|3
GGGAGTGTAGCCACACTCCCTATGCTTGCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14229 GenBank   WP_002317279
Name   Relaxase_EQB51_RS16360_E8290|3 insolico UniProt ID   N2BU79
Length   442 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 442 a.a.        Molecular weight: 50173.57 Da        Isoelectric Point: 9.6754

>WP_002317279.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Terrabacteria group]
MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADIEFEMLLAQAYQKGNNLGHHLIQSFAP
GEATPEQAHEIGKQLAEEVLQGKYPYVLTTHIDKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDR
LCKENGLSVVKPGQSKGKSYAEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFI
SFRAPGQERFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWAKLHN
LKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLIKNISTYKQLWPVAMEY
RNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALKAEYKRLAEEKERLYEKYGEAKKQMQEYG
IIKQNVDGILRMTPGKEWTQEL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(10-240)


  Protein structure


Source ID Structure
AlphaFold DB N2BU79


Auxiliary protein

ID   7866 GenBank   WP_002317278
Name   WP_002317278_E8290|3 insolico UniProt ID   A0A0E2H681
Length   109 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 109 a.a.        Molecular weight: 12486.63 Da        Isoelectric Point: 10.5610

>WP_002317278.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Terrabacteria group]
MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLIDGYIVNMDTSDIRAQTAEIQKIGVNVN
QIAKRLNGMGPVYAQDIEDIKGALAQIWQLQRYILSSQR

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(60-99)


  Protein structure


Source ID Structure
AlphaFold DB A0A0E2H681


T4CP

ID   16489 GenBank   WP_002317293
Name   t4cp2_EQB51_RS16660_E8290|3 insolico UniProt ID   _
Length   602 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 602 a.a.        Molecular weight: 68060.13 Da        Isoelectric Point: 6.2196

>WP_002317293.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Terrabacteria group]
MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHFADGFAAAFANPLPSLHLFDLCIGIAG
AVIVRLVVYCKSKNVKKYRKGMEYGSARWGTAKDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNK
NVLVIGGSGSGKTRFFVKPNLMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSM
KYNPFVYIHSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTLLEMI
NASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISCGARLAPFDIREVRELM
EDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINLLCDRADDVYGGRLPVHVRLILDEFANIG
QIPNFDKLIATIRSREISASIILQSQSQLKAIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETID
SFNTSENRGSQTSHGLNYQKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADK
KNTFDVDRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(140-543)

  Protein structure



No available structure.




Host bacterium

ID   22992 GenBank   NZ_LR135396
Plasmid name   E8290|3 Incompatibility group   -
Plasmid size   63798 bp Coordinate of oriT [Strand]   9217..9246 [-]; 17221..17248 [+]
Host baterium   Enterococcus faecium isolate E8290

Cargo genes

Drug resistance gene   VanHBX, erm(B), aph(3')-III, ant(6)-Ia
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -