Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 122565 |
| Name | oriT1_E7948|3  |
| Organism | Enterococcus faecium isolate E7948 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_LR135359 (26573..26602 [-], 30 nt) |
| oriT length | 30 nt |
| IRs (inverted repeats) | 1..8, 13..20 (GGGAGTGT..ACACTCCC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 30 nt
>oriT1_E7948|3
GGGAGTGTAGCCACACTCCCTATGCTTGCT
GGGAGTGTAGCCACACTCCCTATGCTTGCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14228 | GenBank | WP_002317279 |
| Name | Relaxase_EQB40_RS15330_E7948|3 |
UniProt ID | N2BU79 |
| Length | 442 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 442 a.a. Molecular weight: 50173.57 Da Isoelectric Point: 9.6754
>WP_002317279.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Terrabacteria group]
MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADIEFEMLLAQAYQKGNNLGHHLIQSFAP
GEATPEQAHEIGKQLAEEVLQGKYPYVLTTHIDKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDR
LCKENGLSVVKPGQSKGKSYAEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFI
SFRAPGQERFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWAKLHN
LKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLIKNISTYKQLWPVAMEY
RNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALKAEYKRLAEEKERLYEKYGEAKKQMQEYG
IIKQNVDGILRMTPGKEWTQEL
MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADIEFEMLLAQAYQKGNNLGHHLIQSFAP
GEATPEQAHEIGKQLAEEVLQGKYPYVLTTHIDKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDR
LCKENGLSVVKPGQSKGKSYAEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFI
SFRAPGQERFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWAKLHN
LKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLIKNISTYKQLWPVAMEY
RNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALKAEYKRLAEEKERLYEKYGEAKKQMQEYG
IIKQNVDGILRMTPGKEWTQEL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | N2BU79 |
Auxiliary protein
| ID | 7865 | GenBank | WP_002317278 |
| Name | WP_002317278_E7948|3 |
UniProt ID | A0A0E2H681 |
| Length | 109 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 109 a.a. Molecular weight: 12486.63 Da Isoelectric Point: 10.5610
>WP_002317278.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Terrabacteria group]
MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLIDGYIVNMDTSDIRAQTAEIQKIGVNVN
QIAKRLNGMGPVYAQDIEDIKGALAQIWQLQRYILSSQR
MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLIDGYIVNMDTSDIRAQTAEIQKIGVNVN
QIAKRLNGMGPVYAQDIEDIKGALAQIWQLQRYILSSQR
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E2H681 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 11309..22988
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EQB40_RS15200 | 6433..7128 | + | 696 | WP_002326819 | hypothetical protein | - |
| EQB40_RS16240 | 7175..7411 | - | 237 | WP_306297428 | hypothetical protein | - |
| EQB40_RS15210 | 7509..7688 | + | 180 | WP_001120991 | hypothetical protein | - |
| EQB40_RS15215 | 7819..8088 | + | 270 | WP_000388479 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| EQB40_RS15220 | 8081..8338 | + | 258 | WP_000588503 | Txe/YoeB family addiction module toxin | - |
| EQB40_RS15225 | 8797..9555 | + | 759 | Protein_13 | hypothetical protein | - |
| EQB40_RS15230 | 9801..11000 | + | 1200 | WP_002317296 | DUF6017 domain-containing protein | - |
| EQB40_RS15235 | 11022..11234 | + | 213 | WP_002317295 | DUF5348 domain-containing protein | - |
| EQB40_RS15240 | 11309..11785 | + | 477 | WP_002317294 | PcfB family protein | cd411 |
| EQB40_RS15245 | 11782..12807 | + | 1026 | Protein_17 | type IV secretory system conjugative DNA transfer family protein | - |
| EQB40_RS15250 | 13584..15488 | + | 1905 | WP_127820671 | group II intron reverse transcriptase/maturase | - |
| EQB40_RS15255 | 15601..16380 | + | 780 | Protein_19 | TraG/TraD/VirD4 family protein | - |
| EQB40_RS15260 | 16457..16672 | + | 216 | WP_002317292 | Maff2 family mobile element protein | prgF |
| EQB40_RS15265 | 16764..17627 | + | 864 | WP_002317291 | VirB6/TrbL-like conjugal transfer protein, CD1112 family | prgHa |
| EQB40_RS15270 | 17658..18200 | + | 543 | WP_002317290 | MT-A70 family methyltransferase | - |
| EQB40_RS15275 | 18214..18636 | + | 423 | WP_002370797 | PrgI family protein | prgIa |
| EQB40_RS15280 | 18566..20965 | + | 2400 | WP_002317289 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| EQB40_RS15285 | 20997..22988 | + | 1992 | WP_002382864 | C40 family peptidase | cd419a |
| EQB40_RS15290 | 23011..23262 | + | 252 | WP_002317286 | DUF4315 family protein | - |
| EQB40_RS15295 | 23252..24481 | + | 1230 | WP_002317285 | CD1107 family mobile element protein | - |
| EQB40_RS15300 | 24478..26559 | + | 2082 | WP_002317284 | DNA topoisomerase 3 | - |
Host bacterium
| ID | 22991 | GenBank | NZ_LR135359 |
| Plasmid name | E7948|3 | Incompatibility group | - |
| Plasmid size | 51724 bp | Coordinate of oriT [Strand] | 26573..26602 [-]; 34577..34604 [+] |
| Host baterium | Enterococcus faecium isolate E7948 |
Cargo genes
| Drug resistance gene | VanHBX |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |