Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 122563 |
Name | oriT1_pA12-3 |
Organism | Lactococcus lactis subsp. lactis strain A12 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_LT599052 (21283..21419 [+], 137 nt) |
oriT length | 137 nt |
IRs (inverted repeats) | 21..28, 42..49 (AAAAGGGG..CCCCTTTT) 25..30, 39..44 (GGGGAA..TTCCCC) |
Location of nic site | 104..105 |
Conserved sequence flanking the nic site |
GCTTGCAGTA |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 137 nt
>oriT1_pA12-3
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGCTTTATATGGTCTATTTTGTT
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGCTTTATATGGTCTATTTTGTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 14226 | GenBank | WP_021721723 |
Name | Relaxase_LLA12_RS13010_pA12-3 | UniProt ID | S6EVE0 |
Length | 85 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 85 a.a. Molecular weight: 9713.03 Da Isoelectric Point: 10.2135
>WP_021721723.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus lactis]
MTVTKTIQIKSESQLGRALEYITNAKKTINETLVSGHALNNVHNAEFEMLRTRRFAQKLKGHYSNGKDEV
FAHHIIQSFDPKDKS
MTVTKTIQIKSESQLGRALEYITNAKKTINETLVSGHALNNVHNAEFEMLRTRRFAQKLKGHYSNGKDEV
FAHHIIQSFDPKDKS
Protein domains
No domain identified.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | S6EVE0 |
Host bacterium
ID | 22989 | GenBank | NZ_LT599052 |
Plasmid name | pA12-3 | Incompatibility group | - |
Plasmid size | 42401 bp | Coordinate of oriT [Strand] | 21283..21419 [+]; 30611..30746 [+] |
Host baterium | Lactococcus lactis subsp. lactis strain A12 |
Cargo genes
Drug resistance gene | - |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | AcrID1 |