Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   122556
Name   oriT1_Spyo01|unnamed in_silico
Organism   Streptococcus pyogenes strain Spyo01
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP136949 (2670..2710 [+], 41 nt)
oriT length   41 nt
IRs (inverted repeats)      15..23, 29..37  (TAAAGTATA..TATACTTTA)
 4..9, 12..17  (TTATAT..ATATAA)
Location of nic site      28..29
Conserved sequence flanking the
  nic site    		  GTGTGTATAC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 41 nt

>oriT1_Spyo01|unnamed
CACTTATATGAATATAAAGTATAGTGTGTATACTTTACATG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14221 GenBank   WP_014072567
Name   mobV_R3H37_RS08995_Spyo01|unnamed insolico UniProt ID   G3ER43
Length   500 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 500 a.a.        Molecular weight: 58463.75 Da        Isoelectric Point: 6.0256

>WP_014072567.1 MULTISPECIES: MobV family relaxase [Streptococcus]
MSYLVARMQKMKVGNLGGAYRHNERVFENHSNKDIDTSRSHLNYELTDRDRSVSYEKQIKDYVNEKKSSK
RAIRKDAVLCDEWIITSDKDFFERLDQEQTRAFFETAKNYFAERYGLENIAYASVHLDESTPHMHMGVVP
MVDGKLSSKAVFTREELKAIQEELPKYMNEQGFELSRGQLGSDKKHLSVADYKAKIGKEALNKELLGLGA
PRYWHKEEDRPATAEEIAGYESLASLFAGEEMKLREATLEERFTWLDSHRNDLKGDLSHLEELVDKKIEE
YTRIDSEASERLSELSELNSKVKDREKELRGLESDSERLSDKVVRLEKEHRETTQLLVEQNRNLRKISFQ
DLDRRRIAEDLHEELEKATPKLFGGSFNFTADFVGRLKTFMSEVVEKLEQVINQNEVLRKALEGMKQAKE
RAERELLQEEWKTQRLETENQNLRQENKELKVSKNLLEDIQEVITEKEVSSLNKRLDELREARMASKRGY
EPKRSKGRSI

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-194)


  Protein structure


Source ID Structure
AlphaFold DB G3ER43


Host bacterium

ID   22982 GenBank   NZ_CP136949
Plasmid name   Spyo01|unnamed Incompatibility group   -
Plasmid size   4967 bp Coordinate of oriT [Strand]   2670..2710 [+]; 1343..1380 [+]
Host baterium   Streptococcus pyogenes strain Spyo01

Cargo genes

Drug resistance gene   erm(T)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -