Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 122530
• Name: oriT1_pE165
• Organism: Enterococcus faecalis strain E165
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP089585 (2539..2579 [-], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 15..23, 29..37 (TAAAGTATA..TATACTTTA) 4..9, 12..17 (TTATAT..ATATAA)
• Location of nic site: 28..29
• Conserved sequence flanking the nic site: GTGTGTATAC
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT1_pE165
CACTTATATGAATATAAAGTATAGTGTGTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_LRO92_RS00015_pE165

• ID: 14201
• GenBank: WP_032494831
• Name: mobV_LRO92_RS00015_pE165
• UniProt ID: _
• Length: 403 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 403 a.a.
• Molecular weight: 46964.92 Da
• Isoelectric Point: 9.2169

>WP_032494831.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSFVVARMQKMKSGNLVGVGNHNQRNTDNHSNKDIDVERSYLNYDLVNRTENYKRDIEQFINDNKSSSRA
VRKDAVLINEWIITSDNQFFKDKDDKEIKDFFEQSKDYFAEKFGDENIRYAQVHLDETTPHMHLGIVPFN
AEKKLSAKTVFNRQALQAVQDELPKYLNERGFELERGEKGSERKNLTVPEYKKAKDELKEMTTTLEQRKS
EVLALSNDKTATIKKESLDLKDETKTVKVPSGETIGIGKLRHEFMKNEERKTGNVIISKDKLNALIQGYE
DLYSSNERLKKYAETDLPKEITKVKGKYSELVKEYNDLVRKSNRNIETLEELEKENKSLKNEIKDIYKGF
KAYFKDSKQVTTEQVKTLVGGVVDKVKEFVKGGEFEKIHRNETRTRDRDELSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

No available structure.

Host bacterium

• ID: 22956
• GenBank: NZ_CP089585
• Plasmid name: pE165
• Incompatibility group: -
• Plasmid size: 4244 bp
• Coordinate of oriT [Strand]: 2539..2579 [-]; 3864..3901 [-]
• Host baterium: Enterococcus faecalis strain E165

Cargo genes

• Drug resistance gene: erm(T)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -