Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 122442
• Name: oriT1_pWP5-W18-ESBL-06_1
• Organism: Klebsiella quasipneumoniae strain WP5-W18-ESBL-06
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_AP022113 (2750..2844 [-], 95 nt)
• oriT length: 95 nt
• IRs (inverted repeats): 73..78, 85..90 (AAAAAA..TTTTTT) 73..78, 84..89 (AAAAAA..TTTTTT) 27..34, 37..44 (AGCGTGAT..ATCACGCT) 13..19, 31..37 (TAAATCA..TGATTTA)
• Location of nic site: 55..56
• Conserved sequence flanking the nic site: GGTGTATAGC
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 95 nt

>oriT1_pWP5-W18-ESBL-06_1
TTTTTTTTCTTTTAAATCAGTGAGATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTTGGTAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traI_H7R83_RS26725_pWP5-W18-ESBL-06_1

• ID: 14148
• GenBank: WP_182937522
• Name: traI_H7R83_RS26725_pWP5-W18-ESBL-06_1
• UniProt ID: _
• Length: 518 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 518 a.a.
• Molecular weight: 57714.71 Da
• Isoelectric Point: 10.5610

>WP_182937522.1 TraI/MobA(P) family conjugative relaxase [Klebsiella quasipneumoniae]
MPAFEWVHVQLHQQKGMISLSPPTICNSADKFITTDYLQQCHNLHIHIAINKIHPTRHTIHEPYYPHRTL
ADLCEVLERDYNLERDNHQPRQRGAAARAADMEQHAGVESLVGWIKRECLEEIRSAASWAELHQVMQSNG
LELRARGNGLVIAAGDGTTIKASTLARDLSKPALEKRLGTFEATAERQAQQAQRSYQKKPLRMRVDTTEL
YARYQSEQKGLSAARAEALAEAKRRKDRAVEDAKRKAATKRAAIKLATGGVTKRLLYSQTSARLRNDLEA
IRKQYDQERTKLYQGFQRRQWADWLKQEAMNGDSQALDALRAREAAQGLKGDTLTGQGKAKPGHAPVIDN
ITKKGTIIYRAGKTAVRDDGNRLQVSCGAGGDGVQVALRMAMERYGECLTVNGSPEFKAAVIRAAVDSKM
PITFADPGLEKRRQALIEKARRPGVPPVGHEPPPHRRNGLRNLSQVEGLRIEGEAVQKPAPAPTQKPAGV
SEQEQRKAKLRAEMEAKKARRQGKGRGR

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (42-179)

• Large polyvalent protein-associated domain 7 (LPD7) (347-436)

Protein structure

No available structure.

T4CP

traD_H7R83_RS27330_pWP5-W18-ESBL-06_1

• ID: 16450
• GenBank: WP_080923161
• Name: traD_H7R83_RS27330_pWP5-W18-ESBL-06_1
• UniProt ID: _
• Length: 766 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 766 a.a.
• Molecular weight: 85335.11 Da
• Isoelectric Point: 4.9615

>WP_080923161.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPDDAGSHAGERPELASQPA
PAEVTVSPAPVKAPATTTMPAAEPSARTAEPPVLRVTTVPLIKPKAAAAASTASSAGNPAAVAGGTEQEL
AQQSAEQGQDMLPAGMNEDGVIEDTQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

Protein domains

Predicted by InterproScan.

• F sex factor protein N terminal (TraD_N) (32-128)

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (172-560)

Protein structure

No available structure.

Host bacterium

• ID: 22868
• GenBank: NZ_AP022113
• Plasmid name: pWP5-W18-ESBL-06_1
• Incompatibility group: IncFIA
• Plasmid size: 190538 bp
• Coordinate of oriT [Strand]: 2750..2844 [-]; 37213..37262 [+]
• Host baterium: Klebsiella quasipneumoniae strain WP5-W18-ESBL-06

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: pcoE
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIE9