Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   122440
Name   oriT1_pNUITMVRE1_3 in_silico
Organism   Enterococcus faecium strain NUITM-VRE1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP026776 (19616..19653 [+], 38 nt)
oriT length   38 nt
IRs (inverted repeats)      1..6, 10..15  (CTTTAC..GTAAAG)
Location of nic site      24..25
Conserved sequence flanking the
  nic site    		  GTGTGTTATA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 38 nt

>oriT1_pNUITMVRE1_3
CTTTACGAAGTAAAGTATAGTGTGTTATACTTTACATG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14146 GenBank   WP_002328823
Name   mobV_OO969_RS15935_pNUITMVRE1_3 insolico UniProt ID   O32791
Length   403 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 403 a.a.        Molecular weight: 46815.76 Da        Isoelectric Point: 9.2672

>WP_002328823.1 MULTISPECIES: MobV family relaxase [Lactobacillales]
MSFAVARMQKVKSGNLVGVGNHNQRNTDNHSNKDIDVERSHLNYDLVNRTENYKRDIEQFINDNKSSSRA
VRKDAVLINEWIITSDNPFFKDKDDKEIKDFFDTAKSYFADKFGDNNIRYAQVHLDETTPHMHLGIVPFN
DEHKLSAKTVFNRQALQAVQDELPKYLNERGFELERGEKGSERKNLTVPEYKKAKDELKEITTTLEQRKS
EVLALSNDKAPTIKKESLDLKDETKTVKVPSGETIGIGKLRHEFMKNEERKTGNVIIPESKLNAVIKSYE
ELYKANEKLKNYAETDLPKEITRVKEKYRELAKDYNRLVRKSNQNIETLEELEKENKSLKNEIKGIYKGF
NQFMENTLGATVGQAKKLMNNLMSEVKEFVKGGEFEKIHRNETKTRDRDELSR

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-193)


  Protein structure


Source ID Structure
AlphaFold DB O32791


Host bacterium

ID   22866 GenBank   NZ_AP026776
Plasmid name   pNUITMVRE1_3 Incompatibility group   -
Plasmid size   25968 bp Coordinate of oriT [Strand]   19616..19653 [+]; 21044..21069 [+]
Host baterium   Enterococcus faecium strain NUITM-VRE1

Cargo genes

Drug resistance gene   tet(M), tet(L), cat, erm(B)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -