Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 122391
• Name: oriT_pLAB1000
• Organism: Lentilactobacillus hilgardii
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_025165 (672..708 [-], 37 nt)
• oriT length: 37 nt
• IRs (inverted repeats): 13..21, 28..36 (TAAAGTATA..TATACTTTA) 2..8, 12..18 (ACTTTAT..ATAAAGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 37 nt

>oriT_pLAB1000
CACTTTATAACATAAAGTATAGTGGGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_LCL13_RS00005_pLAB1000

• ID: 14120
• GenBank: WP_033179284
• Name: mobV_LCL13_RS00005_pLAB1000
• UniProt ID: A0A3Q9W295
• Length: 361 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 361 a.a.
• Molecular weight: 41907.05 Da
• Isoelectric Point: 6.9482

>WP_033179284.1 MULTISPECIES: MobV family relaxase [Lactobacillaceae]
MSFAVARMTKLKADNLVGIGNHDQRKTTNHSNEDIDVSHSHLNYDLVAGRTDNFKTDIEAYINENKASKR
AVRKDAVLVNEWIITSDKDFFEQLDEAETRKYFETAKQYFADNYGDENIRYAVVHMDEKTPHMHMGIVPF
DDDKKLSAKRIFNREALQRIQEELPQYLKENGFDVQRGNKNKERKNLSVPEYKAMREELKKIETEKQETQ
AKLADTKKQLDEIKPRDTKKIASKPTLMNKNKVTVDKSDLADLEQRAVTSDAYNFEKIHLEVENHSLRND
LSEAKGRNYELRKENERLQKLVGTLQGIIRNVDEFLHKKLGINLPEKWLERAGLKEPSKKAPESSQERSE
GHHEELDGPSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-194)

Protein structure

• AlphaFold DB: A0A3Q9W295

Host bacterium

• ID: 22817
• GenBank: NC_025165
• Plasmid name: pLAB1000
• Incompatibility group: -
• Plasmid size: 3331 bp
• Coordinate of oriT [Strand]: 672..708 [-]
• Host baterium: Lentilactobacillus hilgardii

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -