Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 122376 |
| Name | oriT1_pECD227_46  |
| Organism | Escherichia fergusonii ECD227 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CM001145 (16764..16844 [-], 81 nt) |
| oriT length | 81 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 81 nt
>oriT1_pECD227_46
GGGGTGCCGGGGCAAAGCCCTGACCAGACAGCAAAAGGAATATCGTCTCGGGTGACGGTATTACATTTGAACATTCTGTCC
GGGGTGCCGGGGCAAAGCCCTGACCAGACAGCAAAAGGAATATCGTCTCGGGTGACGGTATTACATTTGAACATTCTGTCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14113 | GenBank | WP_255121813 |
| Name | Relaxase_ECD227_RS27230_pECD227_46 |
UniProt ID | _ |
| Length | 203 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 203 a.a. Molecular weight: 23407.01 Da Isoelectric Point: 6.2180
>WP_255121813.1 relaxase/mobilization nuclease domain-containing protein, partial [Escherichia fergusonii]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGF
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 7815 | GenBank | WP_032243529 |
| Name | WP_032243529_pECD227_46 |
UniProt ID | _ |
| Length | 87 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 87 a.a. Molecular weight: 9995.49 Da Isoelectric Point: 11.3946
>WP_032243529.1 IncI1-type relaxosome accessory protein NikA, partial [Escherichia fergusonii]
MSDSGVRKKSEVRQKTVVRTLRFSPAEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDR
MSDSGVRKKSEVRQKTVVRTLRFSPAEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDR
Protein domains
No domain identified.
Protein structure
No available structure.
Host bacterium
| ID | 22802 | GenBank | NZ_CM001145 |
| Plasmid name | pECD227_46 | Incompatibility group | IncX1 |
| Plasmid size | 45663 bp | Coordinate of oriT [Strand] | 16764..16844 [-]; 40224..40311 [+] |
| Host baterium | Escherichia fergusonii ECD227 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |