Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 122371
• Name: oriT_K66-2|unnamed8
• Organism: Vagococcus fluvialis strain K66-2
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP122531 (95..135 [-], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT_K66-2|unnamed8
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_QDW48_RS00005_K66-2|unnamed8

• ID: 14108
• GenBank: WP_172689284
• Name: mobV_QDW48_RS00005_K66-2|unnamed8
• UniProt ID: _
• Length: 415 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 415 a.a.
• Molecular weight: 48836.53 Da
• Isoelectric Point: 9.8313

>WP_172689284.1 MULTISPECIES: MobV family relaxase [Bacilli]
MSFAVVRMQKMKSHDLKGIQFHNQRERESKTNEDIDPDRTHENYDLVNDEPIDYNERVKEIIESQKVGTR
KTRKDAVLVNELLVTSDRYFFERLEPDEQKRFFEESYKLFADRYGKQNIAYATVHVDEKTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMQKLGFDLQRGEKGSDREHIETSKFKKQTLEKEIDLLESELKNK
KSELAILSEEVSGEFKIPVKREKKSVEVPTGKRNLLGIEQKKTVMKSTGNVILKDEVFQDLKQKVKAGAV
LQTRVDQLLNTDYAKENQSLKKEVKELRSTNKSLSEENGRLKSTVEHLTNEIESLYVLTKDFLKARTNDL
ESFKELFGVFVGKVKEKAPRGLFVRQHERSEEKKNTFSLQDVLQRDRELREQRKAKRRKSHDLER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

No available structure.

Host bacterium

• ID: 22797
• GenBank: NZ_CP122531
• Plasmid name: K66-2|unnamed8
• Incompatibility group: -
• Plasmid size: 3843 bp
• Coordinate of oriT [Strand]: 95..135 [-]
• Host baterium: Vagococcus fluvialis strain K66-2

Cargo genes

• Drug resistance gene: erm(C)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -