Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   122328
Name   oriT1_N56454|unnamed in_silico
Organism   Enterococcus faecium strain N56454
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP040905 (46544..46746 [+], 203 nt)
oriT length   203 nt
IRs (inverted repeats)      125..130, 143..148  (AAATCC..GGATTT)
 66..72, 83..89  (ACCCCCC..GGGGGGT)
Location of nic site      135..136
Conserved sequence flanking the
  nic site    		  TTTGGTTACA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 203 nt

>oriT1_N56454|unnamed
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGGCAAAAAAATAGTAGAAAATCCTTTGGTTACAAGGGATTTAGAAAATTTCGGTGTATGTCAAATGAGCTTTAAAAGTTGACATACGCCTTTTTGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14082 GenBank   WP_139896911
Name   mobT_FHK65_RS12855_N56454|unnamed insolico UniProt ID   _
Length   401 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 401 a.a.        Molecular weight: 47305.96 Da        Isoelectric Point: 6.7655

>WP_139896911.1 MobT family relaxase [Enterococcus faecium]
MGGISLNEQTWVQHLKEKRLSYGLSQNRLAIATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQHVVEDVLRLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK

  Protein domains


Predicted by InterproScan.

Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N)

(73-161)

Replication initiation factor (Rep_trans)

(169-373)

Helix-turn-helix (HTH_3)

(15-53)


  Protein structure



No available structure.




Host bacterium

ID   22755 GenBank   NZ_CP040905
Plasmid name   N56454|unnamed Incompatibility group   -
Plasmid size   198480 bp Coordinate of oriT [Strand]   46544..46746 [+]; 56261..56298 [+]
Host baterium   Enterococcus faecium strain N56454

Cargo genes

Drug resistance gene   tet(M), tet(L), erm(B), aph(3')-III, ant(6)-Ia, lnu(B), lsa(E), dfrG
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21