Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   122323
Name   oriT1_pBN62-optrA in_silico
Organism   Lactococcus sp. bn62
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP120419 ( 27620..27756 [+], 137 nt)
oriT length   137 nt
IRs (inverted repeats)      21..28, 42..49  (AAAAGGGG..CCCCTTTT)
 25..30, 39..44  (GGGGAA..TTCCCC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 137 nt

>oriT1_pBN62-optrA
CGACACAATCCAAAGGGAATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGTAGTATTTATGGATTTATATGGTCTATTTTGTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14080 GenBank   WP_284071373
Name   Relaxase_P3G65_RS11625_pBN62-optrA insolico UniProt ID   _
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47732.28 Da        Isoelectric Point: 8.1264

>WP_284071373.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus sp. bn62]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFRAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESTTNRFIDTIKQFAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   22750 GenBank   NZ_CP120419
Plasmid name   pBN62-optrA Incompatibility group   -
Plasmid size   28817 bp Coordinate of oriT [Strand]   17199..17335 [+]; 27620..27756 [+]
Host baterium   Lactococcus sp. bn62

Cargo genes

Drug resistance gene   fexA, optrA
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -