Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   122319
Name   oriT1_2022CK-00284|unnamed1 in_silico
Organism   Morganella morganii strain 2022CK-00284
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP132322 (124342..124446 [+], 105 nt)
oriT length   105 nt
IRs (inverted repeats)      56..61, 74..79  (TGGAAT..ATTCCA)
 46..51, 56..61  (ATTCCA..TGGAAT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 105 nt

>oriT1_2022CK-00284|unnamed1
AAATTGACAAATTCCAAACATGGGTTAGCCTAGTGACAAAGCTAGATTCCAATAGTGGAATAATTGGGTTTAGATTCCAGATAGATAGTTATGTGGATAGGAATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14077 GenBank   WP_058662807
Name   mobH_M2O45_RS00915_2022CK-00284|unnamed1 insolico UniProt ID   A0A221KLA3
Length   987 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 987 a.a.        Molecular weight: 109306.91 Da        Isoelectric Point: 4.9372

>WP_058662807.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALHKLFGGRSGAIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWATQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAYRHGIDRYFIRWRDKRHKRHEQFSL
LAWHRIIPAETQEFLSKSGPSIIEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVNEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISQDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGPVKILMLRLESNDLVFTTEPPAAVTGEVVGDVEDAEIE
FVDPEEADEDQAEGDAGLNNDMLAAELEAEKALAGLGFGDAMAMLKSTSDIAEEKPEQEDTESTELPKPD
ASKKGKQQSKPGKARAEKQPQKPEVKEDLSPQDIAKNAPPLANDDPLQALKDVGGGLGDIDFPFDVFNAS
AETACTDATNLAIPDITEPVKQEEQPKPDFVPQEQNSLQNGDFPIFSDSDEPPSWAIEPLPMLTDTPEQT
IPAPDMQHAGKPKELEKDAKTLLSEMLAGYGEASTLLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPEGA
RSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLVDQLSDAVVAAIKDAEASMGGYQEAFELV
SPPGSGVSKNKSAPKQQSRKKTQQQKPEVDAGAGSEQKTKDKSLQQQPKEKQGNVASLVEEQKRKPVQDQ
EKQNVARLPKREAQPVASKPKVEHERELGHVEVRERDEPEVREFEPPKAKTNPKDINEEDFLPSGVTPEK
ALLMLKDMIQKRSGRWLVTPVLEEDGCLVTSVKAFDMIAGENVGISKHILYGLLSRAQRRPLLKKRQGKL
YLEVDKA

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A221KLA3


T4CP

ID   16430 GenBank   WP_000178858
Name   traD_M2O45_RS00920_2022CK-00284|unnamed1 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69248.76 Da        Isoelectric Point: 7.1093

>WP_000178858.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWMAASCAALAVEQVSTMPSEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPEDMWLGSGFLWENRHAQRVFEILKRDWTSIVGKESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVLTHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVKPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNTDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   16431 GenBank   WP_051479468
Name   traC_M2O45_RS00995_2022CK-00284|unnamed1 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92523.78 Da        Isoelectric Point: 5.9204

>WP_051479468.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIITIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDQSVGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTSLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTSKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQHLAQIRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYDDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSQKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSTFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSDGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRKQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 156919..182023

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2O45_RS00905 (M2O45_00910) 152959..153192 - 234 WP_039272424 hypothetical protein -
M2O45_RS00910 (M2O45_00915) 153174..153791 - 618 WP_012766323 hypothetical protein -
M2O45_RS00915 (M2O45_00920) 153959..156922 + 2964 WP_058662807 MobH family relaxase -
M2O45_RS00920 (M2O45_00925) 156919..158784 + 1866 WP_000178858 conjugative transfer system coupling protein TraD virb4
M2O45_RS00925 (M2O45_00930) 158834..159379 + 546 WP_023356350 hypothetical protein -
M2O45_RS00930 (M2O45_00935) 159336..159965 + 630 WP_000743448 DUF4400 domain-containing protein tfc7
M2O45_RS00935 (M2O45_00940) 159976..160422 + 447 WP_039272421 hypothetical protein -
M2O45_RS00940 (M2O45_00945) 160432..160809 + 378 WP_000869260 hypothetical protein -
M2O45_RS00945 (M2O45_00950) 160809..161471 + 663 WP_039272419 hypothetical protein -
M2O45_RS00950 (M2O45_00955) 161806..162153 + 348 WP_039272417 hypothetical protein -
M2O45_RS00955 (M2O45_00960) 162296..162577 + 282 WP_000805626 type IV conjugative transfer system protein TraL traL
M2O45_RS00960 (M2O45_00965) 162574..163200 + 627 WP_039272415 TraE/TraK family type IV conjugative transfer system protein traE
M2O45_RS00965 (M2O45_00970) 163184..164101 + 918 WP_012766326 type-F conjugative transfer system secretin TraK traK
M2O45_RS00970 (M2O45_00975) 164098..165414 + 1317 WP_039272412 TraB/VirB10 family protein traB
M2O45_RS00975 (M2O45_00980) 165411..165989 + 579 WP_039272410 type IV conjugative transfer system lipoprotein TraV traV
M2O45_RS00980 (M2O45_00985) 165993..166385 + 393 WP_012766327 TraA family conjugative transfer protein -
M2O45_RS00985 (M2O45_00990) 166586..172072 + 5487 WP_039272407 Ig-like domain-containing protein -
M2O45_RS00990 (M2O45_00995) 172221..172928 + 708 WP_001259344 DsbC family protein -
M2O45_RS00995 (M2O45_01000) 172925..175372 + 2448 WP_051479468 type IV secretion system protein TraC virb4
M2O45_RS01000 (M2O45_01005) 175387..175704 + 318 WP_000364398 hypothetical protein -
M2O45_RS01005 (M2O45_01010) 175701..176231 + 531 WP_000672456 S26 family signal peptidase -
M2O45_RS01010 (M2O45_01015) 176194..177462 + 1269 WP_039272404 TrbC family F-type conjugative pilus assembly protein traW
M2O45_RS01015 (M2O45_01020) 177459..178136 + 678 WP_039272402 EAL domain-containing protein -
M2O45_RS01020 (M2O45_01025) 178130..179134 + 1005 WP_000911304 TraU family protein traU
M2O45_RS01025 (M2O45_01030) 179285..182023 + 2739 WP_058662805 conjugal transfer mating pair stabilization protein TraN traN
M2O45_RS01030 (M2O45_01035) 182062..182922 - 861 WP_000679674 hypothetical protein -
M2O45_RS01035 (M2O45_01040) 183034..183684 - 651 WP_000796641 hypothetical protein -
M2O45_RS01040 (M2O45_01045) 183976..184296 + 321 WP_012766334 hypothetical protein -
M2O45_RS01045 (M2O45_01050) 184607..184792 + 186 WP_001186892 hypothetical protein -
M2O45_RS01050 (M2O45_01055) 185013..185981 + 969 WP_001553257 AAA family ATPase -
M2O45_RS01055 (M2O45_01060) 185993..186898 + 906 WP_058662804 hypothetical protein -

Host bacterium

ID   22746 GenBank   NZ_CP132322
Plasmid name   2022CK-00284|unnamed1 Incompatibility group   IncA/C
Plasmid size   198157 bp Coordinate of oriT [Strand]   124342..124446 [+]; 158835..158985 [+]
Host baterium   Morganella morganii strain 2022CK-00284

Cargo genes

Drug resistance gene   dfrA14, mph(A), blaSHV-12, blaVIM-1, aph(3')-XV, ant(3'')-Ia, catB2, qacE, sul1, qnrS1, aph(6)-Id, aph(3'')-Ib, sul2, tet(A), aac(6')-Ib-cr, blaOXA-1, aac(3)-IIa, blaCTX-M-15, blaTEM-1B
Virulence gene   -
Metal resistance gene   merD, merA, merC, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -