Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 122268
• Name: oriT_TSM-31|p2
• Organism: Staphylococcus epidermidis strain TSM-31
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP093204 (777..897 [-], 121 nt)
• oriT length: 121 nt
• IRs (inverted repeats): 53..58, 64..69 (TTGGGG..CCCCAA) 22..29, 34..41 (ATTTTTTC..GAAAAAAT) 1..8, 14..21 (AGTGGCTA..TAGCCACT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 121 nt

>oriT_TSM-31|p2
AGTGGCTAGCAATTAGCCACTATTTTTTCGTTAGAAAAAATCCATAAGGGGCTTGGGGATTACCCCCAACAAGCAGGCGCGTCTGCCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_MNU47_RS11865_TSM-31|p2

• ID: 14044
• GenBank: WP_002498904
• Name: Relaxase_MNU47_RS11865_TSM-31|p2
• UniProt ID: A0A0H2VJA7
• Length: 336 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 336 a.a.
• Molecular weight: 38414.55 Da
• Isoelectric Point: 9.8063

>WP_002498904.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Bacillales]
MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKAARELYGKTDGNQGHVIIQSFQPGEVTPE
QCNQLGLALAEKLVPNHQVAVYTHADTNHVHNHIVLNSIDLNTGKKFNNNKQALRDVRAFNDEVCRAQGL
SVPEKDTARLRYTQTEKAIADPNTTSTAQYSWKDDIREAIDQSTATNMEEFQKHLNQHGITIERVTPKSI
TYRHLAENKKVRGRKLGEDYNKGGIQHGFERQIQRRQQQKHKSDTNFQPKRSTSNRHESTERDTGVTQSD
WDQFAQDTNELERRRQAAESARLVDEKARRDREKRVREKPTSRPIINIDRDHGLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-242)

Protein structure

• AlphaFold DB: A0A0H2VJA7

Host bacterium

• ID: 22695
• GenBank: NZ_CP093204
• Plasmid name: TSM-31|p2
• Incompatibility group: -
• Plasmid size: 6585 bp
• Coordinate of oriT [Strand]: 777..897 [-]
• Host baterium: Staphylococcus epidermidis strain TSM-31

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -