Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 122245
• Name: oriT_pVPRE25b
• Organism: Enterococcus faecalis strain RE25
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP128461 (2782..2831 [-], 50 nt)
• oriT length: 50 nt
• IRs (inverted repeats): _
• Location of nic site: 34..35
• Conserved sequence flanking the nic site: GCTTGCAAAA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 50 nt

>oriT_pVPRE25b
AATATCGCAACATGGTACCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_QSV41_RS00005_pVPRE25b

• ID: 14024
• GenBank: WP_174104218
• Name: mobV_QSV41_RS00005_pVPRE25b
• UniProt ID: _
• Length: 559 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 559 a.a.
• Molecular weight: 65084.49 Da
• Isoelectric Point: 8.3220

>WP_174104218.1 MobV family relaxase [Enterococcus faecalis]
MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYDLVNPGSINYREKIKQIIESQRISKR
AVRKDAVLVNEWIITSDTAFFQKNTDTQAFFTDVVAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYE
GRLSSKQVFSRQNLLEIQEELPTYLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQIL
AEKERELHSYEQAIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPNLANVKEKKFSLNGL
KYVLSASDFSAIKSITKDFETLRTQNKHLKIENNTLAQKNKELTQVNHEQELQITDLAYELTLEKEQLTA
FEEKQLNYDQSEQIKQELDKTTQKVSVLETENKRLKDKITDLTYDFSELNEKYQEIKLKFDGLTTYLHER
LGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTYLIDEKGTLFLPPDTGAKGVTMSLNPHFYPLKS
PFYNRETGCYEFVGEYMANGRSRTNTLQLSEEQVLENKAFTFEQSTDRKVWQESKNHSKGLSKNRGLSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (2-191)

Protein structure

No available structure.

Host bacterium

• ID: 22672
• GenBank: NZ_CP128461
• Plasmid name: pVPRE25b
• Incompatibility group: -
• Plasmid size: 4802 bp
• Coordinate of oriT [Strand]: 2782..2831 [-]
• Host baterium: Enterococcus faecalis strain RE25

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -