Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 122229
• Name: oriT1_pEFM9-1
• Organism: Enterococcus faecalis strain M9
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP098744 (34969..35008 [-], 40 nt)
• oriT length: 40 nt
• IRs (inverted repeats): 2..8, 12..18 (ACTTTAC..GTAAAGT)
• Location of nic site: 26..27
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 40 nt

>oriT1_pEFM9-1
CACTTTACGAAGTAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_NDO76_RS13305_pEFM9-1

• ID: 14011
• GenBank: WP_000119405
• Name: mobV_NDO76_RS13305_pEFM9-1
• UniProt ID: F5X340
• Length: 420 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49660.33 Da
• Isoelectric Point: 9.9693

>WP_000119405.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: F5X340

Host bacterium

• ID: 22656
• GenBank: NZ_CP098744
• Plasmid name: pEFM9-1
• Incompatibility group: -
• Plasmid size: 66643 bp
• Coordinate of oriT [Strand]: 34969..35008 [-]; 33553..33578 [-]
• Host baterium: Enterococcus faecalis strain M9

Cargo genes

• Drug resistance gene: cat, tet(L), tet(M), erm(A), optrA, fexA, ant(6)-Ia, aph(3')-III, erm(B), dfrG
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21