Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 122124 |
| Name | oriT_Col440II  |
| Organism | Enterobacter hormaechei subsp. xiangfangensis strain CNCI 200 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JAPZPX010000073 (1220..1277 [+], 58 nt) |
| oriT length | 58 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 58 nt
>oriT_Col440II
GGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCT
GGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13946 | GenBank | WP_310856480 |
| Name | Relaxase_O8F33_RS25500_Col440II |
UniProt ID | _ |
| Length | 225 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 225 a.a. Molecular weight: 25016.50 Da Isoelectric Point: 10.3640
>WP_310856480.1 relaxase/mobilization nuclease domain-containing protein [Enterobacter hormaechei]
MMIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYAKKYTSGVLSFAEAELPPGQ
REQIMASFERVLMPGLDKDQYSILWVEHTDKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTVV
NGRLGLHDPNAPENRRLLVTPSALPETKLEAAQAITRGLLALAHPGSSKRARTSQGRSQRQVLRWSAPRK
TVSALPTRTGGETSD
MMIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYAKKYTSGVLSFAEAELPPGQ
REQIMASFERVLMPGLDKDQYSILWVEHTDKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTVV
NGRLGLHDPNAPENRRLLVTPSALPETKLEAAQAITRGLLALAHPGSSKRARTSQGRSQRQVLRWSAPRK
TVSALPTRTGGETSD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 7750 | GenBank | WP_310856479 |
| Name | WP_310856479_Col440II |
UniProt ID | _ |
| Length | 42 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 42 a.a. Molecular weight: 4689.25 Da Isoelectric Point: 7.0067
>WP_310856479.1 MbeB family mobilization protein [Enterobacter hormaechei]
MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRQTRP
MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRQTRP
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 7751 | GenBank | WP_042863636 |
| Name | WP_042863636_Col440II |
UniProt ID | _ |
| Length | 106 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 106 a.a. Molecular weight: 11738.51 Da Isoelectric Point: 8.5732
>WP_042863636.1 MULTISPECIES: MobC family plasmid mobilization relaxosome protein [Gammaproteobacteria]
MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARAGRLPSISPALLRQLAGMGNNLNQIARKVNAG
GAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDDDR
MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARAGRLPSISPALLRQLAGMGNNLNQIARKVNAG
GAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDDDR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 22551 | GenBank | NZ_JAPZPX010000073 |
| Plasmid name | Col440II | Incompatibility group | Col440II |
| Plasmid size | 1730 bp | Coordinate of oriT [Strand] | 1220..1277 [+] |
| Host baterium | Enterobacter hormaechei subsp. xiangfangensis strain CNCI 200 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |