Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 122052 |
| Name | oriT_2A1|p2A1  |
| Organism | Thauera sp. 2A1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_SSXV01000143 (83678..83780 [+], 103 nt) |
| oriT length | 103 nt |
| IRs (inverted repeats) | 65..70, 73..78 (ACCGGC..GCCGGT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 103 nt
>oriT_2A1|p2A1
GCCTCGCAGAGCAGGATCCCGTTGAGCGCCCCCGGCGCGAATAAGGCCAAAGCGAAGGGGGATGACCGGCTTGCCGGTTAGCCAACTTCGCCCATCCTGCCCG
GCCTCGCAGAGCAGGATCCCGTTGAGCGCCCCCGGCGCGAATAAGGCCAAAGCGAAGGGGGATGACCGGCTTGCCGGTTAGCCAACTTCGCCCATCCTGCCCG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13913 | GenBank | WP_153167894 |
| Name | traI_GH664_RS23325_2A1|p2A1 |
UniProt ID | _ |
| Length | 769 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 769 a.a. Molecular weight: 82987.71 Da Isoelectric Point: 10.8337
>WP_153167894.1 TraI/MobA(P) family conjugative relaxase [Thauera sp. 2A1]
MIAKHVPMRTLARSNFAELVEYITDAQDKTERLGEIRVTNCAAATLPAVIGEVLATQRQNTRAAGDKTFH
LLVSFRPGEAPDAATLQAIEARLAAGLGFAEHQRVSAVHHDTDNVHLHIAINKIHPERRTLHEPFQSYRT
LADLCVTLEREYGLQTDNHVSRRSVAEGRAADMERHAGVESLLGWVRRVCLEDLLGAHSWAQLHQVLSDN
GLVLRPRGNGLIVESRDGTRVKASTLARELSKPALEARLGPFEAPTEVLADGASDGPPSPSPAVGPHYSK
APVRLRIDTTALHVRYQAEQQRSSAQRSEALASARRRKLRAVDDARRANRLWRATIRVADGKGIDKRLLY
AQASRALRERLAQIHTAYARERAVLYDGFRRRTWADWLKHEALQGNAEALAALRAREAAQGLTGNTLAGA
GTGAGANVDPLGPGPDPVQDTVTKKGTILFRAGSSAVRDDGDRLQVSRTLTREGVKVALGLAMARYGGEI
TVCGTPEFKALVIRVAVDARFPLTFADPALEGRRQRLFTQETAHESTEHPERGRADRGRPRGAGARANRY
GSRSDERSGDRPDENRAGAGVDAHARAAGKPHPGRAGGVPPPASRHRLRGLSELGVVRFAGGGEVLLPGD
VPHHLEQRGAAPDHPLRRGVAGAGGLRAGRMTPAQRAAAGRYIAEREEKRHKGIDIPKHALYDGCAGVIF
FAGLRTVDGQPLALFRPGDVPAGAVRGTDVVLVLPVDAATARRLSRVSRGDAVSVTPHGTIQRTGGHSR
MIAKHVPMRTLARSNFAELVEYITDAQDKTERLGEIRVTNCAAATLPAVIGEVLATQRQNTRAAGDKTFH
LLVSFRPGEAPDAATLQAIEARLAAGLGFAEHQRVSAVHHDTDNVHLHIAINKIHPERRTLHEPFQSYRT
LADLCVTLEREYGLQTDNHVSRRSVAEGRAADMERHAGVESLLGWVRRVCLEDLLGAHSWAQLHQVLSDN
GLVLRPRGNGLIVESRDGTRVKASTLARELSKPALEARLGPFEAPTEVLADGASDGPPSPSPAVGPHYSK
APVRLRIDTTALHVRYQAEQQRSSAQRSEALASARRRKLRAVDDARRANRLWRATIRVADGKGIDKRLLY
AQASRALRERLAQIHTAYARERAVLYDGFRRRTWADWLKHEALQGNAEALAALRAREAAQGLTGNTLAGA
GTGAGANVDPLGPGPDPVQDTVTKKGTILFRAGSSAVRDDGDRLQVSRTLTREGVKVALGLAMARYGGEI
TVCGTPEFKALVIRVAVDARFPLTFADPALEGRRQRLFTQETAHESTEHPERGRADRGRPRGAGARANRY
GSRSDERSGDRPDENRAGAGVDAHARAAGKPHPGRAGGVPPPASRHRLRGLSELGVVRFAGGGEVLLPGD
VPHHLEQRGAAPDHPLRRGVAGAGGLRAGRMTPAQRAAAGRYIAEREEKRHKGIDIPKHALYDGCAGVIF
FAGLRTVDGQPLALFRPGDVPAGAVRGTDVVLVLPVDAATARRLSRVSRGDAVSVTPHGTIQRTGGHSR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16303 | GenBank | WP_096453085 |
| Name | t4cp2_GH664_RS23010_2A1|p2A1 |
UniProt ID | _ |
| Length | 855 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 855 a.a. Molecular weight: 94134.97 Da Isoelectric Point: 6.5500
>WP_096453085.1 MULTISPECIES: VirB4 family type IV secretion/conjugal transfer ATPase [unclassified Thauera]
MIAAIAFVVAGLGAALLLILVARIRAVEARVQLRRHRSRDAGLADLLNYAAVVDDGVIVGKNGAFMAAWC
YRGEDNASRTDEAREQVSVRLNQALAGLGSGWMVHVDAVRRPAPGYPERGRSHFPDAVSAAIDEERRRLF
ERLGTMYEGYFVLTLTWFPPVLAQRKFVELMFDDDAIAPDHTARTMNLIAQFRREIAAIESRLSAAVTLT
RLRGEPRVEEDGSTVTHDAFLGWLQCCLTGAHHPVRLPANPMYLDAVIGGQELWGGVVPRIGRQFIQVVA
IEGFPLESAPGLLTALGELPCAYRWSSRFIFMDPHEAVRHLERYRKKWRQKVRGFFDQVFNTHLGPIDQD
ALSMVADAEAAIAEVNSGLVAQGYYTSVVVLMDEDRDALEASARLIEKAINRLGFVARIETINTLDAFLG
SLPGHGVENVRRPLINTMNLADLLPTSSIWTGSPEAPCPLYPSGAPALMHGVTQGATPFRLNLHVRDLGH
TFMFGPTGAGKSAHLALIAAQLRRYAGMSIYAFDKGLSLYALTRAVGGQHYAVASDDGRLAFCPLQFLES
RGDRAWAMEWIETVLALNGVEATPAQRNEIGHAILSMQRSGARTLSEFSVTIQDEAIRQAIRQYTVDGAM
GHLLDAEEDGLALAGFTTFELEELLALGEKYALPVLLYLFRRIERSLSGQPAAILLDEAWVMLGHPVFRA
KIREWLKVLRKANCLVLMATQSLSDAANSGLLDVIVESTATKIFLPNVFARDPDTAALYRRMGLNTRQIE
ILASAVPKRQYYYVSESGHRLYELALGPIALAFVGATDKASVAAIQRLEARVGERWVHEWLAARGLTIND
QRDDGDPVDAIREAA
MIAAIAFVVAGLGAALLLILVARIRAVEARVQLRRHRSRDAGLADLLNYAAVVDDGVIVGKNGAFMAAWC
YRGEDNASRTDEAREQVSVRLNQALAGLGSGWMVHVDAVRRPAPGYPERGRSHFPDAVSAAIDEERRRLF
ERLGTMYEGYFVLTLTWFPPVLAQRKFVELMFDDDAIAPDHTARTMNLIAQFRREIAAIESRLSAAVTLT
RLRGEPRVEEDGSTVTHDAFLGWLQCCLTGAHHPVRLPANPMYLDAVIGGQELWGGVVPRIGRQFIQVVA
IEGFPLESAPGLLTALGELPCAYRWSSRFIFMDPHEAVRHLERYRKKWRQKVRGFFDQVFNTHLGPIDQD
ALSMVADAEAAIAEVNSGLVAQGYYTSVVVLMDEDRDALEASARLIEKAINRLGFVARIETINTLDAFLG
SLPGHGVENVRRPLINTMNLADLLPTSSIWTGSPEAPCPLYPSGAPALMHGVTQGATPFRLNLHVRDLGH
TFMFGPTGAGKSAHLALIAAQLRRYAGMSIYAFDKGLSLYALTRAVGGQHYAVASDDGRLAFCPLQFLES
RGDRAWAMEWIETVLALNGVEATPAQRNEIGHAILSMQRSGARTLSEFSVTIQDEAIRQAIRQYTVDGAM
GHLLDAEEDGLALAGFTTFELEELLALGEKYALPVLLYLFRRIERSLSGQPAAILLDEAWVMLGHPVFRA
KIREWLKVLRKANCLVLMATQSLSDAANSGLLDVIVESTATKIFLPNVFARDPDTAALYRRMGLNTRQIE
ILASAVPKRQYYYVSESGHRLYELALGPIALAFVGATDKASVAAIQRLEARVGERWVHEWLAARGLTIND
QRDDGDPVDAIREAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16304 | GenBank | WP_153167893 |
| Name | t4cp2_GH664_RS23320_2A1|p2A1 |
UniProt ID | _ |
| Length | 634 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 634 a.a. Molecular weight: 68973.08 Da Isoelectric Point: 8.6387
>WP_153167893.1 type IV secretory system conjugative DNA transfer family protein [Thauera sp. 2A1]
MKLEVNNAVGPQVRAGRAPGGKLLPVLAAVSLAGGLQSATQFFAHRFGYPAQLGEHLHHVYAPWAILEWA
SNWSARYPDELLQAASVGMMVSALGLFGVAVAKVVSSNSSRANATLHGSARWAGKTDIEAAGLLPRPRTL
WDVLTGEAACRSAGVYVGGWEDARGRFHYLRHDGPEHVLTYAPTRSGKGVGLVVPTLLSWGASAVVTDLK
GELWALTAGWRKAHARNTVLRFEPATARGSVCWNPLDEIRLETEDAVGDVQNLVTLIVDPSGKGLESHWQ
KTAFALLVGVILHALYKAKADGVSATLPAVDAMLADPNRDVGELWMEMATTGHLDGQPHPAIASAARDMM
DRPEEEAGSVLSTAKSYLALYRDPVVARNVSRSEFRIRDLMHADDPVTLYIVTQPNDKDRLRPLVRILVS
MVVRRLADRMAFAHGRPVAHYRHRLLLMLDEFAALGRLPILQEALAFVAGYGIKCYLICQDLNQLKSRET
GYGPDETITSNCHVQNAYPPNRLETAEHLSRLTGQTTVVKAQVTTSGRRTAAMLGQVSRTFQEVQRPLLT
PDECLRMPGPRKGADGHIEAPGDMVVYVAGFPAIYGKQPLYFKDPVFRARAAVPAPAVTDRLVSFADTGE
AIRL
MKLEVNNAVGPQVRAGRAPGGKLLPVLAAVSLAGGLQSATQFFAHRFGYPAQLGEHLHHVYAPWAILEWA
SNWSARYPDELLQAASVGMMVSALGLFGVAVAKVVSSNSSRANATLHGSARWAGKTDIEAAGLLPRPRTL
WDVLTGEAACRSAGVYVGGWEDARGRFHYLRHDGPEHVLTYAPTRSGKGVGLVVPTLLSWGASAVVTDLK
GELWALTAGWRKAHARNTVLRFEPATARGSVCWNPLDEIRLETEDAVGDVQNLVTLIVDPSGKGLESHWQ
KTAFALLVGVILHALYKAKADGVSATLPAVDAMLADPNRDVGELWMEMATTGHLDGQPHPAIASAARDMM
DRPEEEAGSVLSTAKSYLALYRDPVVARNVSRSEFRIRDLMHADDPVTLYIVTQPNDKDRLRPLVRILVS
MVVRRLADRMAFAHGRPVAHYRHRLLLMLDEFAALGRLPILQEALAFVAGYGIKCYLICQDLNQLKSRET
GYGPDETITSNCHVQNAYPPNRLETAEHLSRLTGQTTVVKAQVTTSGRRTAAMLGQVSRTFQEVQRPLLT
PDECLRMPGPRKGADGHIEAPGDMVVYVAGFPAIYGKQPLYFKDPVFRARAAVPAPAVTDRLVSFADTGE
AIRL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 9016..19598
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GH664_RS22975 (GH664_22975) | 4427..4882 | + | 456 | WP_153167852 | hypothetical protein | - |
| GH664_RS22980 (GH664_22980) | 5076..6512 | + | 1437 | WP_153167853 | IS1182 family transposase | - |
| GH664_RS22985 (GH664_22985) | 6898..7926 | - | 1029 | WP_096453152 | ParB/RepB/Spo0J family partition protein | - |
| GH664_RS22990 (GH664_22990) | 8354..8716 | + | 363 | WP_096453077 | transcriptional regulator | - |
| GH664_RS22995 (GH664_22995) | 9016..9975 | + | 960 | WP_096453079 | P-type conjugative transfer ATPase TrbB | virB11 |
| GH664_RS23000 (GH664_23000) | 9989..10435 | + | 447 | WP_096453081 | conjugal transfer system pilin TrbC | virB2 |
| GH664_RS23005 (GH664_23005) | 10437..10748 | + | 312 | WP_096453083 | conjugal transfer protein TrbD | virB3 |
| GH664_RS23010 (GH664_23010) | 10745..13312 | + | 2568 | WP_096453085 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| GH664_RS23015 (GH664_23015) | 13309..14109 | + | 801 | WP_096453087 | conjugal transfer protein TrbF | virB8 |
| GH664_RS23020 (GH664_23020) | 14125..15021 | + | 897 | WP_153167854 | P-type conjugative transfer protein TrbG | virB9 |
| GH664_RS23025 (GH664_23025) | 15024..15512 | + | 489 | WP_153167855 | conjugal transfer protein TrbH | - |
| GH664_RS23030 (GH664_23030) | 15518..16921 | + | 1404 | WP_153167856 | TrbI/VirB10 family protein | virB10 |
| GH664_RS23035 (GH664_23035) | 16940..17719 | + | 780 | WP_153167857 | P-type conjugative transfer protein TrbJ | virB5 |
| GH664_RS23040 (GH664_23040) | 17737..18012 | + | 276 | WP_153167858 | entry exclusion lipoprotein TrbK | - |
| GH664_RS23045 (GH664_23045) | 18009..19598 | + | 1590 | WP_153167859 | P-type conjugative transfer protein TrbL | virB6 |
| GH664_RS23050 (GH664_23050) | 19710..20288 | + | 579 | WP_153167860 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| GH664_RS23055 (GH664_23055) | 20291..20812 | + | 522 | WP_194270368 | transglycosylase SLT domain-containing protein | - |
| GH664_RS23060 (GH664_23060) | 21241..22800 | - | 1560 | WP_153167861 | hypothetical protein | - |
Host bacterium
| ID | 22479 | GenBank | NZ_SSXV01000143 |
| Plasmid name | 2A1|p2A1 | Incompatibility group | - |
| Plasmid size | 175000 bp | Coordinate of oriT [Strand] | 83678..83780 [+] |
| Host baterium | Thauera sp. 2A1 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merF, merP, merT, merR2 |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIB |