Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   122037
Name   oriT_pCUVET18-1784.3 in_silico
Organism   Serratia nevei strain CUVET18-1784
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP115018 (80781..80862 [-], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_pCUVET18-1784.3
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13907 GenBank   WP_032440569
Name   traI_O8I24_RS27990_pCUVET18-1784.3 insolico UniProt ID   A0A7G3L4G1
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74384.24 Da        Isoelectric Point: 8.3203

>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L4G1


T4CP

ID   16298 GenBank   WP_032440593
Name   trbC_O8I24_RS27855_pCUVET18-1784.3 insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82679.78 Da        Isoelectric Point: 6.3201

>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 49739..71672

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O8I24_RS27840 (O8I24_27830) 45502..46128 - 627 WP_254913868 RepA family replication protein -
O8I24_RS27845 (O8I24_27835) 46703..46954 - 252 WP_113394424 hypothetical protein -
O8I24_RS27850 (O8I24_27840) 46966..47499 - 534 WP_032440594 phospholipase D family protein -
O8I24_RS27855 (O8I24_27845) 47545..49737 - 2193 WP_032440593 F-type conjugative transfer protein TrbC -
O8I24_RS27860 (O8I24_27850) 49739..50860 - 1122 WP_052455213 thioredoxin fold domain-containing protein trbB
O8I24_RS27865 (O8I24_27855) 50841..52259 - 1419 WP_063666705 hypothetical protein trbA
O8I24_RS27870 (O8I24_27860) 52361..52984 - 624 WP_032440592 plasmid IncI1-type surface exclusion protein ExcA -
O8I24_RS27875 (O8I24_27865) 53050..55194 - 2145 WP_032440591 DotA/TraY family protein traY
O8I24_RS27880 (O8I24_27870) 55236..55817 - 582 WP_050597335 conjugal transfer protein TraX -
O8I24_RS27885 (O8I24_27875) 55814..57010 - 1197 WP_032440590 conjugal transfer protein TraW traW
O8I24_RS27890 (O8I24_27880) 57020..60067 - 3048 WP_032440589 conjugal transfer protein traU
O8I24_RS27895 (O8I24_27885) 60077..60640 - 564 WP_045325070 hypothetical protein traT
O8I24_RS27900 (O8I24_27890) 60684..61085 - 402 WP_032440588 DUF6750 family protein traR
O8I24_RS27905 (O8I24_27895) 61141..61674 - 534 WP_032440587 conjugal transfer protein TraQ traQ
O8I24_RS27910 (O8I24_27900) 61684..62403 - 720 WP_050597333 conjugal transfer protein TraP traP
O8I24_RS27915 (O8I24_27905) 62410..63750 - 1341 WP_032440586 conjugal transfer protein TraO traO
O8I24_RS27920 (O8I24_27910) 63754..64857 - 1104 WP_077264204 DotH/IcmK family type IV secretion protein traN
O8I24_RS27925 (O8I24_27915) 64868..65536 - 669 WP_032440585 DotI/IcmL/TraM family protein traM
O8I24_RS27930 (O8I24_27920) 65608..65961 - 354 WP_032440584 hypothetical protein traL
O8I24_RS27935 (O8I24_27925) 65995..69408 - 3414 WP_052145516 LPD7 domain-containing protein -
O8I24_RS27940 (O8I24_27930) 69426..69692 - 267 WP_032440577 IcmT/TraK family protein traK
O8I24_RS27945 (O8I24_27935) 69689..70876 - 1188 WP_077255878 plasmid transfer ATPase TraJ virB11
O8I24_RS27950 (O8I24_27940) 70854..71672 - 819 WP_032440576 type IV secretory system conjugative DNA transfer family protein traI
O8I24_RS27955 (O8I24_27945) 71669..72133 - 465 WP_225546581 DotD/TraH family lipoprotein -
O8I24_RS27960 (O8I24_27950) 72159..72638 - 480 WP_032440574 lytic transglycosylase domain-containing protein -
O8I24_RS27965 (O8I24_27955) 73370..73597 - 228 WP_032440573 hypothetical protein -
O8I24_RS27970 (O8I24_27960) 74207..75049 + 843 WP_032440572 helix-turn-helix domain-containing protein -
O8I24_RS27975 (O8I24_27965) 75109..76038 + 930 WP_032440581 glycosyltransferase family 9 protein -
O8I24_RS27980 (O8I24_27970) 76031..76525 + 495 WP_032440571 molybdopterin-dependent oxidoreductase -

Host bacterium

ID   22464 GenBank   NZ_CP115018
Plasmid name   pCUVET18-1784.3 Incompatibility group   IncFII
Plasmid size   84227 bp Coordinate of oriT [Strand]   80781..80862 [-]
Host baterium   Serratia nevei strain CUVET18-1784

Cargo genes

Drug resistance gene   tet(A), dfrA1, qacE, sul1, lnu(F), aadA2, qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -