Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   122033
Name   oriT_p2017-45-35 in_silico
Organism   Providencia stuartii strain 2017-45-35
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP109734 (151386..151536 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_p2017-45-35
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13904 GenBank   WP_001337757
Name   mobH_OI982_RS22160_p2017-45-35 insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   16294 GenBank   WP_000178856
Name   traD_OI982_RS22165_p2017-45-35 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   16295 GenBank   WP_000637386
Name   traC_OI982_RS22240_p2017-45-35 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 149470..174661

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OI982_RS22150 (OI982_22350) 145495..145728 - 234 WP_001191892 hypothetical protein -
OI982_RS22155 (OI982_22355) 145710..146327 - 618 WP_001249396 hypothetical protein -
OI982_RS22160 (OI982_22360) 146495..149473 + 2979 WP_001337757 MobH family relaxase -
OI982_RS22165 (OI982_22365) 149470..151335 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
OI982_RS22900 (OI982_22370) 151385..151930 + 546 WP_000228720 hypothetical protein -
OI982_RS22170 (OI982_22375) 151887..152516 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
OI982_RS22905 (OI982_22380) 152564..152740 + 177 WP_169342285 hypothetical protein -
OI982_RS22180 (OI982_22385) 152982..153359 + 378 WP_000869261 hypothetical protein -
OI982_RS22185 (OI982_22390) 153359..154021 + 663 WP_001231463 hypothetical protein -
OI982_RS22190 (OI982_22395) 154202..154345 + 144 WP_001275801 hypothetical protein -
OI982_RS22195 (OI982_22400) 154357..154722 + 366 WP_001052531 hypothetical protein -
OI982_RS22200 (OI982_22405) 154867..155148 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
OI982_RS22205 (OI982_22410) 155145..155771 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
OI982_RS22210 (OI982_22415) 155755..156672 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
OI982_RS22215 (OI982_22420) 156672..157985 + 1314 WP_024131605 TraB/VirB10 family protein traB
OI982_RS22220 (OI982_22425) 157982..158560 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
OI982_RS22225 (OI982_22430) 158564..158956 + 393 WP_000479535 TraA family conjugative transfer protein -
OI982_RS22230 (OI982_22435) 159157..164688 + 5532 WP_000606833 Ig-like domain-containing protein -
OI982_RS22235 (OI982_22440) 164837..165544 + 708 WP_001259347 DsbC family protein -
OI982_RS22240 (OI982_22445) 165541..167988 + 2448 WP_000637386 type IV secretion system protein TraC virb4
OI982_RS22245 (OI982_22450) 168003..168320 + 318 WP_000351984 hypothetical protein -
OI982_RS22250 (OI982_22455) 168317..168847 + 531 WP_001010738 S26 family signal peptidase -
OI982_RS22255 (OI982_22460) 168810..170075 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
OI982_RS22260 (OI982_22465) 170072..170743 + 672 WP_001337754 EAL domain-containing protein -
OI982_RS22265 (OI982_22470) 170743..171747 + 1005 WP_043940352 TraU family protein traU
OI982_RS22270 (OI982_22475) 171851..174661 + 2811 WP_001909286 conjugal transfer mating pair stabilization protein TraN traN
OI982_RS22275 (OI982_22480) 174700..175341 - 642 WP_223854615 hypothetical protein -
OI982_RS22280 (OI982_22485) 175683..176324 - 642 WP_000796664 hypothetical protein -
OI982_RS22285 (OI982_22490) 176618..176938 + 321 WP_000547566 hypothetical protein -
OI982_RS22290 (OI982_22495) 177242..177427 + 186 WP_001186917 hypothetical protein -
OI982_RS22295 (OI982_22500) 177647..178615 + 969 WP_000085162 AAA family ATPase -
OI982_RS22300 (OI982_22505) 178626..179534 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   22460 GenBank   NZ_CP109734
Plasmid name   p2017-45-35 Incompatibility group   IncA/C2
Plasmid size   203863 bp Coordinate of oriT [Strand]   151386..151536 [+]
Host baterium   Providencia stuartii strain 2017-45-35

Cargo genes

Drug resistance gene   blaTEM-1D, ant(2'')-Ia, aac(6')-33, qacE, sul1, blaKPC-2, sul2
Virulence gene   -
Metal resistance gene   merR, merT, merP, merC, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -