Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   122030
Name   oriT_pCUVET18-1371.2 in_silico
Organism   Serratia nevei strain CUVET18-1371
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP115011 (59726..59809 [+], 84 nt)
oriT length   84 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 84 nt

>oriT_pCUVET18-1371.2
ACGGGACAGGATATGTTTTGTAGCACCGCCTGCACGCAGTCGGCTCTACAAAACGTCCTGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13902 GenBank   WP_306686620
Name   traI_O8I25_RS27125_pCUVET18-1371.2 insolico UniProt ID   _
Length   640 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 640 a.a.        Molecular weight: 72957.28 Da        Isoelectric Point: 7.5286

>WP_306686620.1 TraI/MobA(P) family conjugative relaxase [Serratia nevei]
MIPIVPEKRRDGRSSFIQLVSYLTLRDEDKPDLPISPENPYVRPSRSKAAIFDRLVGYLDRNAGGDQQTL
LAMFADGTQQVKSGEVVCETNCFSLETASAEMNAVAMQNTRCVDPVYHCILSWREEDTPTDQQIFESARY
CINQLGMADHQYVFAVHRDTDNVHCHIAVNRIHPESYRAANMYKDVDTLHRACRHLELKHGFTPDNGAWK
VNEQQQVVRSQNEFKSIPRKARQLEYYADSESLFSYAVGECRQAIGDIMADPKRLSWQNLHDELNRAGLV
MKQKGEGLAIYSIQDSSLPPIKASSLHPDLTRTCMEPYLGEFTSTLDAVASPKQPEYVYDPRFHARDLMA
RVERRQARAEAREDLKARYQAYKSAWVRPKLDADEIKARYKNMSKRFAWRKAKARVELRDPLLRKLTYHI
IEVERMKAMAALRLAIKEERQAFKAAPENRRLSYRGWVEQQALMHDQAAIAQLRGWSYRLKRSSLTSSVS
LNGIRFAVSDDSKPLAIDGYQTRIMRDGVVQYVQNGVVQLQDRGERIEVSDPQVEDGQHIITGLVIAQSK
SGEEMVLEGDRQYVQQSCELVSWFNEASGTSLPLTETSQRTLAGYGSLPTENSHGTTDASVNERHELDGY
IQRPTGPRPH

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(87-316)


  Protein structure



No available structure.




T4CP

ID   16292 GenBank   WP_306686622
Name   trbC_O8I25_RS27135_pCUVET18-1371.2 insolico UniProt ID   _
Length   726 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 726 a.a.        Molecular weight: 81639.20 Da        Isoelectric Point: 7.7411

>WP_306686622.1 F-type conjugative transfer protein TrbC [Serratia nevei]
MSQPVEVDTRRVRRPLGQTIIQDALLSPIGVQLSLAGAIGLAIHSPASLLLTTPAALLQLMLFGDQPFRM
PLRLPTDIGGMDLTTEREMPKYRNGIGGFFRYVVRTRKYQRAAGVMCLGFARGKALARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNAICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGSDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLAALVYAVYYKCKREKRR
ISQRVIQEYLPLRKLAELYLEAKRDGWHQEAYNPLENYFNTLAGFRIELISRPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDVDIEDILHNDRILCTTIPALELSKGEAANIGKLYISAIRMTMARDLGF
ELEGMMNDVLIVKKYKGNFPFPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYSELGAVEQQGGLITPHFEDAGSNVIREKDRLDLGDLKD
MNPGEGMISFKSALIPSNAIYIQDDDKMTSALPMRINRFVNLDMPTEAELLDANPTLKKKLPPSAQEVET
ILTRLESPPSQTTCNGLMDPVLQSVVALSLDLNHRADVSYTPAQRGILLFEAARVALQQHKRRWKQQPMA
PKPIRVSKDIAKALATSEHHREERYG

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 65074..92928

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O8I25_RS27125 (O8I25_27115) 60190..62112 + 1923 WP_306686620 TraI/MobA(P) family conjugative relaxase -
O8I25_RS27130 (O8I25_27120) 62165..62482 - 318 WP_306686621 hypothetical protein -
O8I25_RS27135 (O8I25_27125) 62837..65017 - 2181 WP_306686622 F-type conjugative transfer protein TrbC -
O8I25_RS27140 (O8I25_27130) 65074..66012 - 939 WP_306686623 hypothetical protein trbB
O8I25_RS27145 (O8I25_27135) 66037..67362 - 1326 WP_306686624 conjugal transfer protein TrbA trbA
O8I25_RS27150 (O8I25_27140) 67508..68086 - 579 WP_306686625 hypothetical protein -
O8I25_RS27155 (O8I25_27145) 68083..70227 - 2145 WP_306686626 DotA/TraY family protein traY
O8I25_RS27160 (O8I25_27150) 70274..70843 - 570 WP_306686627 conjugal transfer protein TraX -
O8I25_RS27165 (O8I25_27155) 70836..72056 - 1221 WP_285142715 conjugal transfer protein TraW traW
O8I25_RS27170 (O8I25_27160) 72186..75260 - 3075 WP_306686628 ATP-binding protein traU
O8I25_RS27175 (O8I25_27165) 75263..75868 - 606 WP_129993777 hypothetical protein traT
O8I25_RS27180 (O8I25_27170) 75929..76333 - 405 WP_129993776 DUF6750 family protein traR
O8I25_RS27185 (O8I25_27175) 76349..76879 - 531 WP_128884978 conjugal transfer protein TraQ traQ
O8I25_RS27190 (O8I25_27180) 76888..77652 - 765 WP_306686629 hypothetical protein traP
O8I25_RS27195 (O8I25_27185) 77652..78866 - 1215 WP_129993774 conjugal transfer protein TraO traO
O8I25_RS27200 (O8I25_27190) 78871..79860 - 990 WP_306686630 DotH/IcmK family type IV secretion protein traN
O8I25_RS27205 (O8I25_27195) 79862..80533 - 672 WP_129993772 DotI/IcmL/TraM family protein traM
O8I25_RS27210 (O8I25_27200) 80623..81102 - 480 WP_129993771 hypothetical protein traL
O8I25_RS27215 (O8I25_27205) 81111..84341 - 3231 WP_306686631 LPD7 domain-containing protein -
O8I25_RS27220 (O8I25_27210) 84355..84654 - 300 WP_128884984 IcmT/TraK family protein traK
O8I25_RS27225 (O8I25_27215) 84651..85811 - 1161 WP_306686632 plasmid transfer ATPase TraJ virB11
O8I25_RS27230 (O8I25_27220) 85825..86613 - 789 WP_285142727 type IV secretory system conjugative DNA transfer family protein traI
O8I25_RS27235 (O8I25_27225) 86610..87068 - 459 WP_285142728 DotD/TraH family lipoprotein -
O8I25_RS27240 (O8I25_27230) 87094..88494 - 1401 WP_306686634 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
O8I25_RS27245 (O8I25_27235) 88491..89159 - 669 WP_306686635 A24 family peptidase -
O8I25_RS27250 (O8I25_27240) 89159..89644 - 486 WP_306686637 lytic transglycosylase domain-containing protein virB1
O8I25_RS27255 (O8I25_27245) 89699..90289 - 591 WP_285142732 type 4 pilus major pilin -
O8I25_RS27260 (O8I25_27250) 90326..91441 - 1116 WP_306686638 type II secretion system F family protein -
O8I25_RS27265 (O8I25_27255) 91438..92928 - 1491 WP_094887940 GspE/PulE family protein virB11
O8I25_RS27270 (O8I25_27260) 92934..93506 - 573 WP_094887939 type IV pilus biogenesis protein PilP -
O8I25_RS27275 (O8I25_27265) 93493..94791 - 1299 WP_094887938 type 4b pilus protein PilO2 -
O8I25_RS27280 (O8I25_27270) 94795..96426 - 1632 WP_306686639 PilN family type IVB pilus formation outer membrane protein -
O8I25_RS27285 (O8I25_27275) 96448..96870 - 423 WP_285142738 type IV pilus biogenesis protein PilM -
O8I25_RS27290 (O8I25_27280) 96870..97850 - 981 WP_306686640 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   22457 GenBank   NZ_CP115011
Plasmid name   pCUVET18-1371.2 Incompatibility group   -
Plasmid size   99795 bp Coordinate of oriT [Strand]   59726..59809 [+]
Host baterium   Serratia nevei strain CUVET18-1371

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -