Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   122026
Name   oriT_pCUVET19-891.2 in_silico
Organism   Enterobacter hormaechei strain CUVET19-891
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP114987 (32658..32781 [+], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      101..106, 119..124  (TTTAAT..ATTAAA)
 91..99, 113..121  (AATAATGTA..TACATTATT)
 90..95, 107..112  (AAATAA..TTATTT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
 3..10, 15..22  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_pCUVET19-891.2
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTTTTTGAATCATTAGCTTATGTTTTAAATAATGTATTTTAATTTATTTTACATTATTAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13899 GenBank   WP_015059021
Name   traI_O8I28_RS24350_pCUVET19-891.2 insolico UniProt ID   A0A0X8XR69
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 192132.90 Da        Isoelectric Point: 6.0788

>WP_015059021.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Gammaproteobacteria]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAENRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLPEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNIYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDVQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDVMTVVVPGRAEPASLPVSDSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDKATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISHQKSALNTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFADLGREIDA
QIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETPDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISRDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPAQEQAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTSDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQVRERAAITERETALPESVLREPQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(969-1156)

DNA helicase TraI (TraI_C)

(1434-1557)

TrwC relaxase (TrwC)

(10-283)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)


  Protein structure



No available structure.




Auxiliary protein

ID   7723 GenBank   WP_000124981
Name   WP_000124981_pCUVET19-891.2 insolico UniProt ID   A0A630FTW9
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14542.59 Da        Isoelectric Point: 5.0278

>WP_000124981.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Gammaproteobacteria]
MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLELGLRVYDAQMERKESAFNQTEFNKLL
LECVVKTQSTVAKILGIESLSPHVSGNPKFEYASMVDDIREKVSVEMDRFFPKNDDE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A630FTW9

ID   7724 GenBank   WP_001254386
Name   WP_001254386_pCUVET19-891.2 insolico UniProt ID   A0A3Z6KJB5
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 9005.11 Da        Isoelectric Point: 10.1422

>WP_001254386.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Gammaproteobacteria]
MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVTEESES
TFKEL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(14-61)


  Protein structure


Source ID Structure
AlphaFold DB A0A3Z6KJB5


T4CP

ID   16289 GenBank   WP_015059012
Name   traC_O8I28_RS24240_pCUVET19-891.2 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99393.22 Da        Isoelectric Point: 6.3465

>WP_015059012.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGVPFCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAA
TQFPLPEGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKKKQARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(290-446)

TraG P-loop domain (P-loop_TraG)

(467-771)

TraC protein (TraC_F_IV)

(38-276)

  Protein structure



No available structure.



ID   16290 GenBank   WP_000009363
Name   traD_O8I28_RS24345_pCUVET19-891.2 insolico UniProt ID   _
Length   741 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 741 a.a.        Molecular weight: 84259.95 Da        Isoelectric Point: 5.0504

>WP_000009363.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMA
AYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 32086..60274

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O8I28_RS24135 (O8I28_24120) 28058..28492 + 435 WP_000845953 conjugation system SOS inhibitor PsiB -
O8I28_RS24140 (O8I28_24125) 28489..29208 + 720 WP_001276217 plasmid SOS inhibition protein A -
O8I28_RS24145 (O8I28_24130) 29430..29579 + 150 Protein_38 plasmid maintenance protein Mok -
O8I28_RS24150 (O8I28_24135) 29521..29646 + 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
O8I28_RS24155 (O8I28_24140) 29965..30261 - 297 Protein_40 hypothetical protein -
O8I28_RS24160 (O8I28_24145) 30561..30857 + 297 WP_001272251 hypothetical protein -
O8I28_RS24165 (O8I28_24150) 30968..31789 + 822 WP_001234445 DUF932 domain-containing protein -
O8I28_RS24170 (O8I28_24155) 32086..32733 - 648 WP_015059008 transglycosylase SLT domain-containing protein virB1
O8I28_RS24175 (O8I28_24160) 33010..33393 + 384 WP_000124981 conjugal transfer relaxosome DNA-binding protein TraM -
O8I28_RS24180 (O8I28_24165) 33584..34270 + 687 WP_015059009 PAS domain-containing protein -
O8I28_RS24185 (O8I28_24170) 34364..34591 + 228 WP_001254386 conjugal transfer relaxosome protein TraY -
O8I28_RS24190 (O8I28_24175) 34625..34990 + 366 WP_021519752 type IV conjugative transfer system pilin TraA -
O8I28_RS24195 (O8I28_24180) 35005..35316 + 312 WP_000012106 type IV conjugative transfer system protein TraL traL
O8I28_RS24200 (O8I28_24185) 35338..35904 + 567 WP_000399794 type IV conjugative transfer system protein TraE traE
O8I28_RS24205 (O8I28_24190) 35891..36619 + 729 WP_001230787 type-F conjugative transfer system secretin TraK traK
O8I28_RS24210 (O8I28_24195) 36619..38046 + 1428 WP_032297072 F-type conjugal transfer pilus assembly protein TraB traB
O8I28_RS24215 (O8I28_24200) 38036..38626 + 591 WP_000002778 conjugal transfer pilus-stabilizing protein TraP -
O8I28_RS24220 (O8I28_24205) 38613..38810 + 198 WP_001324648 conjugal transfer protein TrbD -
O8I28_RS24225 (O8I28_24210) 38822..39073 + 252 WP_001038341 conjugal transfer protein TrbG -
O8I28_RS24230 (O8I28_24215) 39070..39585 + 516 WP_000809902 type IV conjugative transfer system lipoprotein TraV traV
O8I28_RS24235 (O8I28_24220) 39720..39941 + 222 WP_001278692 conjugal transfer protein TraR -
O8I28_RS24240 (O8I28_24225) 40101..42728 + 2628 WP_015059012 type IV secretion system protein TraC virb4
O8I28_RS24245 (O8I28_24230) 42725..43111 + 387 WP_015059013 type-F conjugative transfer system protein TrbI -
O8I28_RS24250 (O8I28_24235) 43108..43740 + 633 WP_001203735 type-F conjugative transfer system protein TraW traW
O8I28_RS24255 (O8I28_24240) 43737..44729 + 993 WP_000830838 conjugal transfer pilus assembly protein TraU traU
O8I28_RS24260 (O8I28_24245) 44756..45064 + 309 WP_000412447 hypothetical protein -
O8I28_RS24265 (O8I28_24250) 45127..45648 + 522 WP_015059014 hypothetical protein -
O8I28_RS24270 (O8I28_24255) 45675..46313 + 639 WP_000087532 type-F conjugative transfer system pilin assembly protein TrbC trbC
O8I28_RS24275 (O8I28_24260) 46310..46681 + 372 WP_000056336 hypothetical protein -
O8I28_RS24280 (O8I28_24265) 46707..47129 + 423 WP_001229309 HNH endonuclease -
O8I28_RS24285 (O8I28_24270) 47126..48976 + 1851 WP_015059015 type-F conjugative transfer system mating-pair stabilization protein TraN traN
O8I28_RS24290 (O8I28_24275) 49003..49260 + 258 WP_053913405 conjugal transfer protein TrbE -
O8I28_RS24295 (O8I28_24280) 49253..49996 + 744 WP_015059017 type-F conjugative transfer system pilin assembly protein TraF traF
O8I28_RS24300 (O8I28_24285) 50157..50393 + 237 WP_000270010 type-F conjugative transfer system pilin chaperone TraQ -
O8I28_RS24305 (O8I28_24290) 50380..50925 + 546 WP_000059817 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
O8I28_RS24310 (O8I28_24295) 50915..51229 + 315 WP_001384255 P-type conjugative transfer protein TrbJ -
O8I28_RS24315 (O8I28_24300) 51183..51575 + 393 WP_000126338 F-type conjugal transfer protein TrbF -
O8I28_RS24320 (O8I28_24305) 51562..52935 + 1374 WP_000944325 conjugal transfer pilus assembly protein TraH traH
O8I28_RS24325 (O8I28_24310) 52932..55748 + 2817 WP_015059018 conjugal transfer mating-pair stabilization protein TraG traG
O8I28_RS24330 (O8I28_24315) 55781..56302 + 522 WP_000632668 conjugal transfer entry exclusion protein TraS -
O8I28_RS24335 (O8I28_24320) 56327..57058 + 732 WP_021559024 conjugal transfer complement resistance protein TraT -
O8I28_RS24340 (O8I28_24325) 57261..57998 + 738 WP_015059020 hypothetical protein -
O8I28_RS24345 (O8I28_24330) 58049..60274 + 2226 WP_000009363 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   22453 GenBank   NZ_CP114987
Plasmid name   pCUVET19-891.2 Incompatibility group   IncFII
Plasmid size   69933 bp Coordinate of oriT [Strand]   32658..32781 [+]
Host baterium   Enterobacter hormaechei strain CUVET19-891

Cargo genes

Drug resistance gene   blaTEM-1B, rmtB
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -