Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121970 |
| Name | oriT_pCRY  |
| Organism | Yersinia pestis biovar Microtus str. 91001 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NC_005814 (13452..15157 [+], 1706 nt) |
| oriT length | 1706 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 1706 nt
>oriT_pCRY
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGAAACTTAAAGTAATCATGTTGGCGGCAATAGTTACCGGATGTTCATCGCCGCCACCTCCCGTTCCTGTAGAGTGGGATAAAGCCGCCGACCCATTAAACACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTTCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAGTCGCTCATTCAACACGAATTGTCGTCACTGCACAATCCGGTACTGATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGTGTTATTGAATACCAGCCTGTCTTTAATTGTCTGACATGCAGAGAAACCACTATTTATTTCTCTCGCTAATCCATTTAAGCAACACAATCTAATAAGGATTTACCATGAAAAAAACCGTTATCGCGGCGGCTGTATTATGCCTGACCGCAGGAATGCCGATTTATTCCTTTGCTGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCTACGGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATCAACGCATTTAAAAAACACCATCGTTTTAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGCAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGATATGAGAGGTTCGAAATGATAAACGAAGATTTATTCATTAAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCCCTAGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGCTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAGGTTGACGCAAAAAAATTAAGACGCTATGCCGAAGGCAAAGGCACAAAAATTGATGATGCGTCCTACAGTGAAGCACTGGAAGCTGAAAGGGAGTGCAGGGCTGAAATATACCGCGAGCAGATGTAATCACACAAAACAAAAGTGCATTGTACAATACACAGACTAACAGGCGACAGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGTACGCGGAAAGAAAATTCAATTTCGGAGAAATTGCTAATTTTCAGGAACCGCGCCCACCTGCGAACATTCGCCCACAGCGAATTGAGCAGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGGTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGTAATATAACCCGCCCCAGCGGGGGATATTACCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCGATTTTTTCGAGCTTGCGAGGAAAAATAGGCTAAACGCAGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCAAGACGGTGTGCCGCCTTTGACCTTGGGGGTTTTGGCTCGGCGTTCAGCCGGGACATTACCCCCATGTGTCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGGCACACGCCGT
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGAAACTTAAAGTAATCATGTTGGCGGCAATAGTTACCGGATGTTCATCGCCGCCACCTCCCGTTCCTGTAGAGTGGGATAAAGCCGCCGACCCATTAAACACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTTCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAGTCGCTCATTCAACACGAATTGTCGTCACTGCACAATCCGGTACTGATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGTGTTATTGAATACCAGCCTGTCTTTAATTGTCTGACATGCAGAGAAACCACTATTTATTTCTCTCGCTAATCCATTTAAGCAACACAATCTAATAAGGATTTACCATGAAAAAAACCGTTATCGCGGCGGCTGTATTATGCCTGACCGCAGGAATGCCGATTTATTCCTTTGCTGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCTACGGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATCAACGCATTTAAAAAACACCATCGTTTTAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGCAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGATATGAGAGGTTCGAAATGATAAACGAAGATTTATTCATTAAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCCCTAGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGCTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAGGTTGACGCAAAAAAATTAAGACGCTATGCCGAAGGCAAAGGCACAAAAATTGATGATGCGTCCTACAGTGAAGCACTGGAAGCTGAAAGGGAGTGCAGGGCTGAAATATACCGCGAGCAGATGTAATCACACAAAACAAAAGTGCATTGTACAATACACAGACTAACAGGCGACAGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGTACGCGGAAAGAAAATTCAATTTCGGAGAAATTGCTAATTTTCAGGAACCGCGCCCACCTGCGAACATTCGCCCACAGCGAATTGAGCAGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGGTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGTAATATAACCCGCCCCAGCGGGGGATATTACCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCGATTTTTTCGAGCTTGCGAGGAAAAATAGGCTAAACGCAGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCAAGACGGTGTGCCGCCTTTGACCTTGGGGGTTTTGGCTCGGCGTTCAGCCGGGACATTACCCCCATGTGTCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGGCACACGCCGT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16209 | GenBank | WP_016603709 |
| Name | t4cp2_YP_RS21500_pCRY |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103309.26 Da Isoelectric Point: 7.4627
>WP_016603709.1 VirB3 family type IV secretion system protein [Yersinia pestis]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHQDSGNPYADEVSRLYYASVGSFR
RNRLFLTVCYRPFVSLEKAERKRMKDRQKLKELDGALLEMLEIKSTLDTALARYGARSLGTFTEGNMVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEIKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGYAMALMRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEINIRALGGRYYRVISGEPTGWNPFSLPATKRNINFIKQLVKILC
TRNGATITPRDERRLNDAVNAVMSDEPEYRRFGITRLREQLPEPATKEAQENGLDIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHQDSGNPYADEVSRLYYASVGSFR
RNRLFLTVCYRPFVSLEKAERKRMKDRQKLKELDGALLEMLEIKSTLDTALARYGARSLGTFTEGNMVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEIKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGYAMALMRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEINIRALGGRYYRVISGEPTGWNPFSLPATKRNINFIKQLVKILC
TRNGATITPRDERRLNDAVNAVMSDEPEYRRFGITRLREQLPEPATKEAQENGLDIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16210 | GenBank | WP_041175934 |
| Name | t4cp2_YP_RS21565_pCRY |
UniProt ID | _ |
| Length | 578 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 578 a.a. Molecular weight: 65641.81 Da Isoelectric Point: 7.2144
>WP_041175934.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIFTAAGLIFHIAARRLLAGEIDNLKHRMIKKSKLERNT
RTDVRKVKELLPDSIEYNPLDYIDLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILLGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYENGMTLRDLFNTDFVQSLREDVPAFCGELEEIALVNSINAKNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKVDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTSTELPRATKMFQVQVKKENIELLSFEYK
QATPEKNESQEHKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIFTAAGLIFHIAARRLLAGEIDNLKHRMIKKSKLERNT
RTDVRKVKELLPDSIEYNPLDYIDLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILLGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYENGMTLRDLFNTDFVQSLREDVPAFCGELEEIALVNSINAKNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKVDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTSTELPRATKMFQVQVKKENIELLSFEYK
QATPEKNESQEHKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3591..17110
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| YP_RS21465 (pCRY01) | 739..1440 | - | 702 | WP_016603711 | RepA family replication protein | - |
| YP_RS21470 (pCRY03) | 1901..2257 | - | 357 | WP_011172003 | helix-turn-helix transcriptional regulator | - |
| YP_RS21475 (pCRY04) | 2508..2963 | + | 456 | WP_011172004 | transcription termination/antitermination NusG family protein | - |
| YP_RS21480 (pCRY05) | 3035..3250 | + | 216 | WP_004178170 | hypothetical protein | - |
| YP_RS21485 (pCRY06) | 3255..3473 | + | 219 | WP_011172006 | hypothetical protein | - |
| YP_RS25585 | 3470..3592 | - | 123 | WP_016603710 | hypothetical protein | - |
| YP_RS21490 (pCRY07) | 3591..4301 | + | 711 | WP_011172007 | lytic transglycosylase domain-containing protein | virB1 |
| YP_RS21495 (pCRY08) | 4298..4603 | + | 306 | WP_011172008 | TrbC/VirB2 family protein | virB2 |
| YP_RS21500 (pCRY09) | 4616..7354 | + | 2739 | WP_016603709 | VirB3 family type IV secretion system protein | virb4 |
| YP_RS21505 (pCRY10) | 7372..8076 | + | 705 | WP_011172010 | type IV secretion system protein | - |
| YP_RS21510 (pCRY11) | 8090..8317 | + | 228 | WP_011172011 | EexN family lipoprotein | - |
| YP_RS21515 (pCRY12) | 8328..9401 | + | 1074 | WP_011172012 | type IV secretion system protein | virB6 |
| YP_RS21525 (pCRY13) | 9622..10305 | + | 684 | WP_004178111 | type IV secretion system protein | virB8 |
| YP_RS21530 (pCRY14) | 10302..11210 | + | 909 | WP_011172013 | P-type conjugative transfer protein VirB9 | virB9 |
| YP_RS21535 (pCRY15) | 11254..12504 | + | 1251 | WP_011172014 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| YP_RS21540 (pCRY16) | 12494..13519 | + | 1026 | WP_000125558 | P-type DNA transfer ATPase VirB11 | virB11 |
| YP_RS21545 (pCRY17) | 13516..13914 | + | 399 | WP_000592251 | cag pathogenicity island Cag12 family protein | - |
| YP_RS21550 (pCRY18) | 13950..14255 | + | 306 | WP_011172015 | hypothetical protein | - |
| YP_RS21555 (pCRY19) | 14289..14594 | + | 306 | WP_011172016 | hypothetical protein | - |
| YP_RS25420 (pCRY20) | 14705..14908 | + | 204 | WP_226984135 | hypothetical protein | - |
| YP_RS21565 (pCRY22) | 15374..17110 | + | 1737 | WP_041175934 | type IV secretory system conjugative DNA transfer family protein | virb4 |
| YP_RS21570 (pCRY23) | 17119..17874 | + | 756 | WP_016603568 | MobC family replication-relaxation protein | - |
| YP_RS21575 (pCRY24) | 17911..18384 | + | 474 | WP_011172021 | thermonuclease family protein | - |
| YP_RS21580 (pCRY25) | 18445..18786 | + | 342 | WP_011172022 | hypothetical protein | - |
| YP_RS21585 (pCRY26) | 18824..19057 | - | 234 | WP_004178129 | hypothetical protein | - |
| YP_RS21590 (pCRY27) | 19098..19745 | - | 648 | WP_004178130 | ParA family protein | - |
| YP_RS21595 (pCRY28) | 19866..20648 | - | 783 | WP_016603533 | SprT-like domain-containing protein | - |
| YP_RS21600 (pCRY29) | 21026..21307 | - | 282 | WP_000780222 | helix-turn-helix transcriptional regulator | - |
| YP_RS21605 | 21288..21617 | - | 330 | WP_000493286 | type II toxin-antitoxin system RelE/ParE family toxin | - |
Host bacterium
| ID | 22397 | GenBank | NC_005814 |
| Plasmid name | pCRY | Incompatibility group | IncFII |
| Plasmid size | 21742 bp | Coordinate of oriT [Strand] | 13452..15157 [+] |
| Host baterium | Yersinia pestis biovar Microtus str. 91001 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |