Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121966 |
| Name | oriT_pAtA74a_b  |
| Organism | Agrobacterium tumefaciens strain A74a |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP116695 (82320..82355 [+], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 22..27, 31..36 (CGTCGC..GCGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_pAtA74a_b
GGCTCCAAGGGCGCAATTATACGTCGCGATGCGACG
GGCTCCAAGGGCGCAATTATACGTCGCGATGCGACG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13843 | GenBank | WP_082186424 |
| Name | traA_G6M15_RS25685_pAtA74a_b |
UniProt ID | _ |
| Length | 1100 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1100 a.a. Molecular weight: 123702.41 Da Isoelectric Point: 10.0835
>WP_082186424.1 Ti-type conjugative transfer relaxase TraA [Agrobacterium tumefaciens]
MAIAHFSASIVSRGSGRSAVLSAAYRHCAKMEYEREARTIDYTRKQGLLHEEFVLPADAPKWVRSLIADR
SVAGASEAFWNKVEAFEKRADAQLARDLTIALPLELSAEQNIALVRDFVEQHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLSDDGFGSKKVAVIGDDGQPVRTKSGKILYELWAGSTDDFNVLRDGWFERLNHHLAIGG
VDIRIDGRSYEKQGIDLEPTIHLGVGAKAIERKAEQQGIRPELERIELNEQRRSENTRRILRNPAIVLDL
ITREKSVFDERDIAKVLHRYIDDPALFQQLMLKIILNRQVLRLQRDTIDFATGEKLPARYSTRAMIRLEA
TMARQAMWLSNREGRGVSHSALDAAFKRHERLSDEQKAAIERIAGPARIAAVVGRAGAGKTTMMKAAREA
WELTGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWKRGRDLLDDKTIFVMDEAGMVASKQMAGFVD
AVVRAGAKIVLVGDPEQLQPIEAGAAFRAIVDRVGYAELETIYRQREDWMRKASLDLARGNVEKALSAYG
ANVRITGERLKAEAVERLIADWNHDYDQTKTTLILAHLRRDVRMLNVMAREKLVERGMVGEGHLFRTADG
ERRFDAGDQIVFLKNEGSLGVKNGMIGHVVEAAANRIVATVGERDQRRQVIVEPRFYNNLDHGYATTIHK
SQGTTVDRVKVLASLSLDRHLTYVAMTRHREDLQLYYGTRSFSFNGGLAKVLSRKNAKETTLDYARGQLY
REALRFSENRGLHIVQVARTLVRDRLDWTLRQKAKLVDLSERLAAFAARLGFTQSPTTQTKKEAPPMVAG
IKTFAGSVADTVGDKLGADPTLKRQWEEVSARFAYVFADPETAFRAMKFDAVLVDKEMAKQILQKLEAEP
ASLGPLKGKSGILATRTERQARRVAEINVPALKRDLEQYLRMRETATQRLQTDEQVLRQRLSIDIPALSP
AARVVLERVRDAIDRNDLPAAMAYALSNRETKLEIDGFNKAITERFGERTLLSNAAREPSGKLYEELSKG
MKPEQKEELKQAWPVMRTAQQLAAHERTSHSLRQAEEQRLTQRQTPVIKQ
MAIAHFSASIVSRGSGRSAVLSAAYRHCAKMEYEREARTIDYTRKQGLLHEEFVLPADAPKWVRSLIADR
SVAGASEAFWNKVEAFEKRADAQLARDLTIALPLELSAEQNIALVRDFVEQHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLSDDGFGSKKVAVIGDDGQPVRTKSGKILYELWAGSTDDFNVLRDGWFERLNHHLAIGG
VDIRIDGRSYEKQGIDLEPTIHLGVGAKAIERKAEQQGIRPELERIELNEQRRSENTRRILRNPAIVLDL
ITREKSVFDERDIAKVLHRYIDDPALFQQLMLKIILNRQVLRLQRDTIDFATGEKLPARYSTRAMIRLEA
TMARQAMWLSNREGRGVSHSALDAAFKRHERLSDEQKAAIERIAGPARIAAVVGRAGAGKTTMMKAAREA
WELTGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWKRGRDLLDDKTIFVMDEAGMVASKQMAGFVD
AVVRAGAKIVLVGDPEQLQPIEAGAAFRAIVDRVGYAELETIYRQREDWMRKASLDLARGNVEKALSAYG
ANVRITGERLKAEAVERLIADWNHDYDQTKTTLILAHLRRDVRMLNVMAREKLVERGMVGEGHLFRTADG
ERRFDAGDQIVFLKNEGSLGVKNGMIGHVVEAAANRIVATVGERDQRRQVIVEPRFYNNLDHGYATTIHK
SQGTTVDRVKVLASLSLDRHLTYVAMTRHREDLQLYYGTRSFSFNGGLAKVLSRKNAKETTLDYARGQLY
REALRFSENRGLHIVQVARTLVRDRLDWTLRQKAKLVDLSERLAAFAARLGFTQSPTTQTKKEAPPMVAG
IKTFAGSVADTVGDKLGADPTLKRQWEEVSARFAYVFADPETAFRAMKFDAVLVDKEMAKQILQKLEAEP
ASLGPLKGKSGILATRTERQARRVAEINVPALKRDLEQYLRMRETATQRLQTDEQVLRQRLSIDIPALSP
AARVVLERVRDAIDRNDLPAAMAYALSNRETKLEIDGFNKAITERFGERTLLSNAAREPSGKLYEELSKG
MKPEQKEELKQAWPVMRTAQQLAAHERTSHSLRQAEEQRLTQRQTPVIKQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16204 | GenBank | WP_082186427 |
| Name | traG_G6M15_RS25670_pAtA74a_b |
UniProt ID | _ |
| Length | 647 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 647 a.a. Molecular weight: 69664.70 Da Isoelectric Point: 9.6991
>WP_082186427.1 Ti-type conjugative transfer system protein TraG [Agrobacterium tumefaciens]
MTANRLLLAILPAAIMVAAMILTSGMDHRLAALGTTAQAKLMLGRTGLALPYVAATAISVVALFAANGST
NIKAAGLSVLAGSAVVIIIAVAREAIRLAGIASNVPAGQTILAYADPATMIGVGIAFIGSVFALRVAIKG
NAAFAMAAPKRIGGRRAIHGEADWMKMQEAAKLFPEHGGIVIGERYRVDRDSVATMQFRADEPHSWGAGG
RSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVSEHRRQAGRKVIVLD
PTASGVGFNALDWIGRHGNTKEEDIVAVATWIMTDNPRTASARDDFFRASAMQLLTALIADACLSGHTDE
KDQTLRQVRANLSEPEPKLRARLTKIYEGSQSDFVKENVSVFVNMTPETFSGVYANAVKETHWLSYPNYA
GLVSGDSFSTDDLSNGETDIFIALDLKVLEAHPGFARVVIGSLLNAIYNRNGDVKGRTLFLLDEVARLGY
LRILETARDAGRKYGITLTMIFQSLGQMREAYGGRDATSKWFESASWISFAAINDPDTADYISKRCGDTT
VEVDQTNRSSGMKGSSRSRSKQLNRRPLILPHEVLRMRADEQIVFTSGNAPLRCGRAVWFRREDMKASVG
ENRFHKRSSATHEPAVR
MTANRLLLAILPAAIMVAAMILTSGMDHRLAALGTTAQAKLMLGRTGLALPYVAATAISVVALFAANGST
NIKAAGLSVLAGSAVVIIIAVAREAIRLAGIASNVPAGQTILAYADPATMIGVGIAFIGSVFALRVAIKG
NAAFAMAAPKRIGGRRAIHGEADWMKMQEAAKLFPEHGGIVIGERYRVDRDSVATMQFRADEPHSWGAGG
RSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVSEHRRQAGRKVIVLD
PTASGVGFNALDWIGRHGNTKEEDIVAVATWIMTDNPRTASARDDFFRASAMQLLTALIADACLSGHTDE
KDQTLRQVRANLSEPEPKLRARLTKIYEGSQSDFVKENVSVFVNMTPETFSGVYANAVKETHWLSYPNYA
GLVSGDSFSTDDLSNGETDIFIALDLKVLEAHPGFARVVIGSLLNAIYNRNGDVKGRTLFLLDEVARLGY
LRILETARDAGRKYGITLTMIFQSLGQMREAYGGRDATSKWFESASWISFAAINDPDTADYISKRCGDTT
VEVDQTNRSSGMKGSSRSRSKQLNRRPLILPHEVLRMRADEQIVFTSGNAPLRCGRAVWFRREDMKASVG
ENRFHKRSSATHEPAVR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16205 | GenBank | WP_174012472 |
| Name | t4cp2_G6M15_RS25870_pAtA74a_b |
UniProt ID | _ |
| Length | 820 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 820 a.a. Molecular weight: 91450.32 Da Isoelectric Point: 5.9268
>WP_174012472.1 conjugal transfer protein TrbE [Agrobacterium tumefaciens]
MVALKSFRHSGPSFADLVPYAGLVDNSVILLKDGSLMAGWYFAGPDSESSTDAEHNEVSRQINAILSRLG
SGWMIQVEAVRVPTGDYPTEEASHFPDPVTRAIDAERRAHFQKERGHYESRHALILTWRPPEPRRSGLTR
YVYSDVGSRSATYADKALETFLTSIREVEQYLANVVSIQRMMTRETPERGGHRVARYDELFQFIRFCITG
ENHPVRLPEISMYLDWLVTAELQHGLTPLVENRFLGVVAIDGLPAESWPGILNSLDLMPLTYRWSSRFVF
LDAEEARARLERTRKKWQQKVRPFFDQLFQTQSRSLDQDAMLMVAETEDAIAEASSQLVAYGYYTPVIIL
FDEDQSRLQEKCEAVRRLIQAEGFGARIETINATDAFLGSLPGVSYANIREPLINTRNLADLIPLNSIWS
GSPIAPCPFYPVGAPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLSLIAAQFRRYAGAQIFA
FDKGGSMLPLTLGLGGDHYQIGGDAQEAGEGRALSFCPLAELSTDGDRAFASEWIEMLVALQGVTITPDY
RNAISRQVGLMAESRGRSLSDFVSGVQMREIKDALHHYTVDGPMGQLLDAEADGLALGAFQCFEIEELMN
MGERNLVPVLTYLFRRIEKRLTGAPSLIILDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSISDA
ERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATAIPKRDYYVVSPEGRRLFDMAL
GPVALSFVGASGKEDLKRIRALHLEHGASWPLHWLQQRGIAHADTLIPIH
MVALKSFRHSGPSFADLVPYAGLVDNSVILLKDGSLMAGWYFAGPDSESSTDAEHNEVSRQINAILSRLG
SGWMIQVEAVRVPTGDYPTEEASHFPDPVTRAIDAERRAHFQKERGHYESRHALILTWRPPEPRRSGLTR
YVYSDVGSRSATYADKALETFLTSIREVEQYLANVVSIQRMMTRETPERGGHRVARYDELFQFIRFCITG
ENHPVRLPEISMYLDWLVTAELQHGLTPLVENRFLGVVAIDGLPAESWPGILNSLDLMPLTYRWSSRFVF
LDAEEARARLERTRKKWQQKVRPFFDQLFQTQSRSLDQDAMLMVAETEDAIAEASSQLVAYGYYTPVIIL
FDEDQSRLQEKCEAVRRLIQAEGFGARIETINATDAFLGSLPGVSYANIREPLINTRNLADLIPLNSIWS
GSPIAPCPFYPVGAPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLSLIAAQFRRYAGAQIFA
FDKGGSMLPLTLGLGGDHYQIGGDAQEAGEGRALSFCPLAELSTDGDRAFASEWIEMLVALQGVTITPDY
RNAISRQVGLMAESRGRSLSDFVSGVQMREIKDALHHYTVDGPMGQLLDAEADGLALGAFQCFEIEELMN
MGERNLVPVLTYLFRRIEKRLTGAPSLIILDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSISDA
ERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATAIPKRDYYVVSPEGRRLFDMAL
GPVALSFVGASGKEDLKRIRALHLEHGASWPLHWLQQRGIAHADTLIPIH
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 117437..127111
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G6M15_RS25825 (G6M15_25825) | 114649..115728 | - | 1080 | WP_174012457 | efflux RND transporter periplasmic adaptor subunit | - |
| G6M15_RS25830 (G6M15_25830) | 115993..117036 | + | 1044 | WP_174012459 | AraC family transcriptional regulator | - |
| G6M15_RS25835 (G6M15_25835) | 117437..118744 | - | 1308 | WP_174012461 | IncP-type conjugal transfer protein TrbI | virB10 |
| G6M15_RS25840 (G6M15_25840) | 118756..119175 | - | 420 | WP_236777255 | conjugal transfer protein TrbH | - |
| G6M15_RS25845 (G6M15_25845) | 119235..120089 | - | 855 | WP_174012463 | P-type conjugative transfer protein TrbG | virB9 |
| G6M15_RS25850 (G6M15_25850) | 120107..120769 | - | 663 | WP_174012465 | conjugal transfer protein TrbF | virB8 |
| G6M15_RS25855 (G6M15_25855) | 120785..121975 | - | 1191 | WP_174012467 | P-type conjugative transfer protein TrbL | virB6 |
| G6M15_RS25860 (G6M15_25860) | 121969..122196 | - | 228 | WP_174012468 | entry exclusion protein TrbK | - |
| G6M15_RS25865 (G6M15_25865) | 122193..123002 | - | 810 | WP_174012470 | P-type conjugative transfer protein TrbJ | virB5 |
| G6M15_RS25870 (G6M15_25870) | 122974..125436 | - | 2463 | WP_174012472 | conjugal transfer protein TrbE | virb4 |
| G6M15_RS25875 (G6M15_25875) | 125448..125747 | - | 300 | WP_174012474 | conjugal transfer protein TrbD | virB3 |
| G6M15_RS25880 (G6M15_25880) | 125740..126114 | - | 375 | WP_236777256 | TrbC/VirB2 family protein | virB2 |
| G6M15_RS25885 (G6M15_25885) | 126140..127111 | - | 972 | WP_174012478 | P-type conjugative transfer ATPase TrbB | virB11 |
| G6M15_RS25890 (G6M15_25890) | 127108..127743 | - | 636 | WP_174012480 | acyl-homoserine-lactone synthase | - |
Host bacterium
| ID | 22393 | GenBank | NZ_CP116695 |
| Plasmid name | pAtA74a_b | Incompatibility group | - |
| Plasmid size | 128118 bp | Coordinate of oriT [Strand] | 82320..82355 [+] |
| Host baterium | Agrobacterium tumefaciens strain A74a |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |