Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121959
• Name: oriT_pK188-5-C
• Organism: Enterococcus faecium strain K188-5
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP116531 (10519..10556 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_pK188-5-C
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_PML77_RS13930_pK188-5-C

• ID: 13838
• GenBank: WP_000119405
• Name: mobV_PML77_RS13930_pK188-5-C
• UniProt ID: F5X340
• Length: 420 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49660.33 Da
• Isoelectric Point: 9.9693

>WP_000119405.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: F5X340

Host bacterium

• ID: 22386
• GenBank: NZ_CP116531
• Plasmid name: pK188-5-C
• Incompatibility group: -
• Plasmid size: 18533 bp
• Coordinate of oriT [Strand]: 10519..10556 [-]
• Host baterium: Enterococcus faecium strain K188-5

Cargo genes

• Drug resistance gene: tet(L), tet(M)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -